Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil

dc.contributor.authorMurata, Fernando Henrique Antunes
dc.contributor.authorFerreira, Marina Neves [UNESP]
dc.contributor.authorPereira-Chioccola, Vera Lucia
dc.contributor.authorSpegiorin, Lígia Cosentino Junqueira Franco
dc.contributor.authorMeira-Strejevitch, Cristina da Silva
dc.contributor.authorGava, Ricardo
dc.contributor.authorSilveira‐Carvalho, Aparecida Perpétuo
dc.contributor.authorde Mattos, Luiz Carlos
dc.contributor.authorBrandão de Mattos, Cinara Cássia
dc.contributor.institutionSão José do Rio Preto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Adolfo Lutz – IAL
dc.contributor.institutionUnited States Department of Agriculture
dc.date.accessioned2018-12-11T16:48:05Z
dc.date.available2018-12-11T16:48:05Z
dc.date.issued2017-09-01
dc.description.abstractToxoplasmosis during pregnancy can have severe consequences. The use of sensitive and specific serological and molecular methods is extremely important for the correct diagnosis of the disease. We compared the ELISA and ELFA serological methods, conventional PCR (cPCR), Nested PCR and quantitative PCR (qPCR) in the diagnosis of Toxoplasma gondii infection in pregnant women without clinical suspicion of toxoplasmosis (G1 = 94) and with clinical suspicion of toxoplasmosis (G2 = 53). The results were compared using the Kappa index, and the sensitivity, specificity, positive predictive value and negative predictive value were calculated. The results of the serological methods showed concordance between the ELISA and ELFA methods even though ELFA identified more positive cases than ELISA. Molecular methods were discrepant with cPCR using B22/23 primers having greater sensitivity and lower specificity compared to the other molecular methods.en
dc.description.affiliationFaculdade de Medicina de São José do Rio Preto – FAMERP São José do Rio Preto
dc.description.affiliationInstituto de Biociências Letras e Ciência Exatas Universidade Estadual Paulista “Júlio de Mesquista Filho” – IBILCE-UNESP São José do Rio Preto
dc.description.affiliationLaboratório de Biologia Molecular de Parasitas e Fungos do Centro de Parasitologia e Micologia Instituto Adolfo Lutz – IAL
dc.description.affiliationHospital da Criança e Maternidade São José do Rio Preto
dc.description.affiliationFAMERP Toxoplasma Research Group São José do Rio Preto
dc.description.affiliationAnimal Parasitic Diseases Laboratory Beltsville Agricultural Research Center Agricultural Research Service United States Department of Agriculture
dc.description.affiliationUnespInstituto de Biociências Letras e Ciência Exatas Universidade Estadual Paulista “Júlio de Mesquista Filho” – IBILCE-UNESP São José do Rio Preto
dc.format.extent13-19
dc.identifierhttp://dx.doi.org/10.1016/j.diagmicrobio.2017.06.004
dc.identifier.citationDiagnostic Microbiology and Infectious Disease, v. 89, n. 1, p. 13-19, 2017.
dc.identifier.doi10.1016/j.diagmicrobio.2017.06.004
dc.identifier.file2-s2.0-85021855778.pdf
dc.identifier.issn1879-0070
dc.identifier.issn0732-8893
dc.identifier.scopus2-s2.0-85021855778
dc.identifier.urihttp://hdl.handle.net/11449/169895
dc.language.isoeng
dc.relation.ispartofDiagnostic Microbiology and Infectious Disease
dc.relation.ispartofsjr1,157
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectGestational toxoplasmosis
dc.subjectMolecular diagnosis
dc.subjectPolymerase chain reaction
dc.subjectSerology
dc.subjectToxoplasma gondii
dc.subjectToxoplasmosis
dc.titleEvaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazilen
dc.typeArtigo

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