Large-scale discovery of previously undetected microRNAs specific to human liver

dc.contributor.authorMinatel, Brenda C.
dc.contributor.authorMartinez, Victor D.
dc.contributor.authorNg, Kevin W.
dc.contributor.authorSage, Adam P.
dc.contributor.authorTokar, Tomas
dc.contributor.authorMarshall, Erin A.
dc.contributor.authorAnderson, Christine
dc.contributor.authorEnfield, Katey S. S.
dc.contributor.authorStewart, Greg L.
dc.contributor.authorReis, Patricia P. [UNESP]
dc.contributor.authorJurisica, Igor
dc.contributor.authorLam, Wan L.
dc.contributor.institutionBritish Columbia Cancer Research Centre
dc.contributor.institutionUniversity Health Network
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.abstractMicroRNAs (miRNAs) are crucial regulators of gene expression in normal development and cellular homeostasis. While miRNA repositories contain thousands of unique sequences, they primarily contain molecules that are conserved across several tissues, largely excluding lineage and tissue-specific miRNAs. By analyzing small non-coding RNA sequencing data for abundance and secondary RNA structure, we discovered 103 miRNA candidates previously undescribed in liver tissue. While expression of some of these unannotated sequences is restricted to non-malignant tissue, downregulation of most of the sequences was detected in liver tumors, indicating their importance in the maintenance of liver homeostasis. Furthermore, target prediction revealed the involvement of the unannotated miRNA candidates in fatty-acid metabolism and tissue regeneration, which are key pathways in liver biology. Here, we provide a comprehensive analysis of the undiscovered liver miRNA transcriptome, providing new resources for a deeper exploration of organ-specific biology and disease.en
dc.description.affiliationDepartment of Integrative Oncology British Columbia Cancer Research Centre
dc.description.affiliationKrembil Research Institute University Health Network
dc.description.affiliationFaculty of Medicine São Paulo State University (UNESP)
dc.description.affiliationUnespFaculty of Medicine São Paulo State University (UNESP)
dc.description.sponsorshipCanada Foundation for Innovation
dc.description.sponsorshipCanadian Institutes of Health Research
dc.description.sponsorshipUniversity of British Columbia
dc.description.sponsorshipIdCanada Foundation for Innovation: 225404
dc.description.sponsorshipIdCanada Foundation for Innovation: 30865
dc.identifier.citationHuman Genomics, v. 12, n. 1, 2018.
dc.relation.ispartofHuman Genomics
dc.rights.accessRightsAcesso aberto
dc.subjectLiver cancer
dc.subjectNon-coding RNA
dc.subjectNovel miRNA
dc.subjectTissue specificity
dc.titleLarge-scale discovery of previously undetected microRNAs specific to human liveren