Contrasting effects of nitric oxide and corticotropin-releasing factor within the dorsal periaqueductal gray on defensive behavior and nociception in mice

Página do item simplificado
dc.contributor.authorMiguel, T. T. [UNESP]
dc.contributor.authorGomes, K. S. [UNESP]
dc.contributor.authorNunes-de-Souza, R. L. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T18:57:25Z
dc.date.available2022-04-28T18:57:25Z
dc.date.issued2012-04-01
dc.description.abstractThe anxiogenic and antinociceptive effects produced by glutamate N-methyl-D-aspartate receptor activation within the dorsal periaqueductal gray (dPAG) matter have been related to nitric oxide (NO) production, since injection of NO synthase (NOS) inhibitors reverses these effects. dPAG corticotropin-releasing factor receptor (CRFr) activation also induces anxiety-like behavior and antinociception, which, in turn, are selectively blocked by local infusion of the CRF type 1 receptor (CRFrl) antagonist, NBI 27914 [5-chloro-4-(N-(cyclopropyl)methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)aminopyridine]. Here, we determined whether i) the blockade of the dPAG by CRFrl attenuates the anxiogenic/antinociceptive effects induced by local infusion of the NO donor, NOC-9 [6-(2-hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-hexanamine], and ii) the anxiogenic/antinociceptive effects induced by intra-dPAG CRF are prevented by local infusion of Nu-propyl-L-arginine (NPLA), a neuronal NOS inhibitor, in mice. Male Swiss mice (12 weeks old, 25-35 g, N = 8-14/group) were stereotaxically implanted with a 7-mm cannula aimed at thedPAG. Intra-dPAG NOC-9 (75 nmol) produced defensive-like behavior (jumping and running) and antinociception (assessed by the formalin test). Both effects were reversed by prior local infusion of NBI 27914 (2 nmol). Conversely, intra-dPAG NPLA (0.4 nmol) did not modify the anxiogenic/antinociceptive effects of CRF (150 pmol). These results suggest that CRFrl plays an important role in the defensive behavior and antinociception produced by NO within the dPAG. In contrast, the anxiogenic and antinociceptive effects produced by intra-dPAG CRF are not related to NO synthesis in this limbic midbrain structure.en
dc.description.affiliationUniversidade Federal de São Carlos Universidade Estadual Paulista, Araraquara, SP
dc.description.affiliationLaboratório de Farmacologia Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, Araraquara, SP
dc.description.affiliationUnespUniversidade Federal de São Carlos Universidade Estadual Paulista, Araraquara, SP
dc.description.affiliationUnespLaboratório de Farmacologia Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, Araraquara, SP
dc.format.extent299-307
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2012007500043
dc.identifier.citationBrazilian Journal of Medical and Biological Research, v. 45, n. 4, p. 299-307, 2012.
dc.identifier.doi10.1590/S0100-879X2012007500043
dc.identifier.issn0100-879X
dc.identifier.issn1678-4510
dc.identifier.scopus2-s2.0-84859588114
dc.identifier.urihttp://hdl.handle.net/11449/219784
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.sourceScopus
dc.subjectAntinociception
dc.subjectCorticotropin-releasing factor
dc.subjectDefensive behavior
dc.subjectMice
dc.subjectNitric oxide
dc.subjectPeriaqueductal gray
dc.titleContrasting effects of nitric oxide and corticotropin-releasing factor within the dorsal periaqueductal gray on defensive behavior and nociception in miceen
dc.typeArtigo

Arquivos

Coleções

Artigos