Mechanism and control of Genipa americana seed germination

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dc.contributor.authorEster Queiroz, Sue Ellen
dc.contributor.authorAmaral da Silva, Edvaldo Aparecido [UNESP]
dc.contributor.authorDavide, Antonio Claudio
dc.contributor.authorJose, Anderson Cleiton
dc.contributor.authorSilva, Anderson Tadeu
dc.contributor.authorResende Fraiz, Ana Carla
dc.contributor.authorRocha Faria, Jose Marcio
dc.contributor.authorHilhorst, Henk W. M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Fed Goiano
dc.contributor.institutionUniversidade Federal de Lavras (UFLA)
dc.contributor.institutionWageningen Univ
dc.date.accessioned2014-05-20T13:20:07Z
dc.date.available2014-05-20T13:20:07Z
dc.date.issued2012-03-01
dc.description.abstractGenipa americana (Rubiaceae) is important for restoration of riparian forest in the Brazilian Cerrado. The objective was to characterize the mechanism and control of germination of G. americana to support uniform seedling production. Morphology and morphometrics of seeds, embryo and endosperm were assessed by light and scanning electron microscopy during germination. Imbibition and germination curves were generated and over the same time interval endosperm digestion and resistance were measured by puncture force analysis and activity assay of endo-beta-mannanase (EBM) in water and in abscisic acid (ABA). The gene encoding for EBM was partially cloned and its expression monitored by quantitative real-time-polymerase chain reaction. Embryos displayed growth prior to radicle protrusion. A two-phase increase in EBM activity coincided with the two stages of weakening of the micropylar endosperm. The second stage also coincided with growth of the embryo prior to radicle protrusion. Enzyme activity was initiated in the micropylar endosperm but spread to the lateral endosperm. ABA completely inhibited germination by inhibiting embryo growth, the second stage of weakening and expression of the EBM gene, but EBM activity was not significantly inhibited. This suggests that a specific isoform of the enzyme is involved in endosperm weakening. EBM may cause a general softening of micropylar endosperm cell walls, allowing the embryo to puncture the endosperm as the driving force of the decrease in puncture force.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agron, Dept Prod Vegetal, BR-18603970 Botucatu, SP, Brazil
dc.description.affiliationInst Fed Goiano, BR-75790000 Urutai, Goias, Brazil
dc.description.affiliationUniversidade Federal de Lavras (UFLA), Dept Ciencias Florestais, Lab Sementes Florestais, BR-37200000 Lavras, MG, Brazil
dc.description.affiliationUniversidade Federal de Lavras (UFLA), Dept Quim, Lab Cent Biol, BR-37200000 Lavras, MG, Brazil
dc.description.affiliationWageningen Univ, Lab Plant Physiol, NL-6708 PB Wageningen, Netherlands
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agron, Dept Prod Vegetal, BR-18603970 Botucatu, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipIFS-International Foundation for Science-Sweden
dc.description.sponsorshipIdCAPES: WUR 007/09
dc.description.sponsorshipIdFAPEMIG: CRA APQ-3615.5-04/07
dc.description.sponsorshipIdIFS: D/3834-1
dc.format.extent263-276
dc.identifierhttp://dx.doi.org/10.1111/j.1399-3054.2011.01554.x
dc.identifier.citationPhysiologia Plantarum. Malden: Wiley-blackwell, v. 144, n. 3, p. 263-276, 2012.
dc.identifier.doi10.1111/j.1399-3054.2011.01554.x
dc.identifier.issn0031-9317
dc.identifier.urihttp://hdl.handle.net/11449/5497
dc.identifier.wosWOS:000300504600006
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofPhysiologia Plantarum
dc.relation.ispartofjcr2.580
dc.relation.ispartofsjr1,257
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleMechanism and control of Genipa americana seed germinationen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.orcid0000-0002-5642-8001[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agronômicas, Botucatupt
unesp.departmentProdução Vegetalpt
unesp.departmentProdução e Melhoramento Vegetal - FCApt

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Botucatu - FCA - Faculdade de Ciências Agronômicas