Purification and characterization of polygalacturonase produced by thermophilic Thermoascus aurantiacus CBMAI-756 in submerged fermentation

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dc.contributor.authorMartins, Eduardo Silva
dc.contributor.authorSilva, Denis
dc.contributor.authorLeite, Rodrigo S. R.
dc.contributor.authorGomes, Eleni
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:23:18Z
dc.date.available2014-05-20T15:23:18Z
dc.date.issued2007-04-01
dc.description.abstractAn extracellular polygalacturonase was isolated from 5-day culture filtrates of Thermoascus aurantiacus CBMAI-756 and purified by gel filtration and ion-exchange chromatography. The enzyme was maximally active at pH 5.5 and 60-65 degrees C. The apparent K (m) with citrus pectin was 1.46 mg/ml and the V (max) was 2433.3 mu mol/min/mg. The apparent molecular weight of the enzyme was 30 kDa. The enzyme was 100% stable at 50 degrees C for 1 h and showed a half-life of 10 min at 60 degrees C. Polygalacturonase was stable at pH 5.0-5.5 and maintained 33% of initial activity at pH 9.0. Metal ions, such as Zn+2, Mn+2, and Hg+2, inhibited 50, 75 and 100% of enzyme activity. The purified polygalacturonase was shown to be an endo/exo-enzyme, releasing mono, di and tri-galacturonic acids within 10 min of hydrolysis.en
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Letras & Ciências Exatas, BR-15054000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Letras & Ciências Exatas, BR-15054000 São Paulo, Brazil
dc.format.extent291-299
dc.identifierhttp://dx.doi.org/10.1007/s10482-006-9114-6
dc.identifier.citationAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. Dordrecht: Springer, v. 91, n. 3, p. 291-299, 2007.
dc.identifier.doi10.1007/s10482-006-9114-6
dc.identifier.issn0003-6072
dc.identifier.lattes709124742851920
dc.identifier.orcid0000-0003-0935-1387
dc.identifier.urihttp://hdl.handle.net/11449/34119
dc.identifier.wosWOS:000244950700009
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
dc.relation.ispartofjcr1.588
dc.relation.ispartofsjr0,834
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectpolygalacturonasept
dc.subjectpurificationpt
dc.subjectThermoascus aurantiacuspt
dc.titlePurification and characterization of polygalacturonase produced by thermophilic Thermoascus aurantiacus CBMAI-756 in submerged fermentationen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes709124742851920[4]
unesp.author.orcid0000-0003-1468-5752[2]
unesp.author.orcid0000-0002-0837-5072[3]
unesp.author.orcid0000-0003-0935-1387[4]

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