Human bcl-2 expression, cleaved caspase-3, and KSHV LANA-1 in Kaposi sarcoma lesions

dc.contributor.authorda Silva, Suzane Ramos
dc.contributor.authorBacchi, Maura M.
dc.contributor.authorBacchi, Carlos E.
dc.contributor.authorOliveira, Deilson Elgui de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionConsultoria Patol
dc.date.accessioned2014-05-20T13:36:57Z
dc.date.available2014-05-20T13:36:57Z
dc.date.issued2007-11-01
dc.description.abstractWe aimed to evaluate the frequency of Kaposi sarcoma (KS)-associated herpesvirus (KSHV) infection in KS lesions in patients from Brazil. In addition, expression of human bcl-2, cleaved caspase-3, and KSHV latency-associated nuclear antigen (LANA)-1 in tumors was evaluated using inummohistochemical analysis. We studied 64 KS cases, classified as follows: classical, 20 (31 %); iatrogenic, 2 (3 %); AIDS-associated, 25 (39%); and not otherwise specified (lack of information about HIV status), 17 (27%). KSHV was detected by polymerase chain reaction (PCR) in 61 cases (95%); 40 cases (63%) were KSHV+ by PCR and immunohistochemical analysis for LANA-L Immunoexpression of bcl-2 was detected in 47 cases (73%). Only a few cells in 15 cases (23%) of KS had demonstrable immunostaining for cleaved caspase-3. These results further support the association of KSHV with all KS forms. Cleaved caspase-3 in KS tumors was infrequent, which may reflect the inhibition of apoptosis owing to bcl-2 overexpression observed in the majority of KS tumors.en
dc.description.affiliationUniv Estadual Paulista, Fac Med Botucatu, Fac Med Botucatu, Dept Patol,Lab Patol Mol, BR-18618970 São Paulo, Brazil
dc.description.affiliationConsultoria Patol, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Botucatu, Fac Med Botucatu, Dept Patol,Lab Patol Mol, BR-18618970 São Paulo, Brazil
dc.format.extent794-802
dc.identifierhttp://dx.doi.org/10.1309/TFU2FXK3AP0C9R2X
dc.identifier.citationAmerican Journal of Clinical Pathology. Chicago: Amer Soc Clinical Pathology, v. 128, n. 5, p. 794-802, 2007.
dc.identifier.doi10.1309/TFU2FXK3AP0C9R2X
dc.identifier.issn0002-9173
dc.identifier.lattes5240998569868081
dc.identifier.urihttp://hdl.handle.net/11449/12732
dc.identifier.wosWOS:000250310000012
dc.language.isoeng
dc.publisherAmer Soc Clinical Pathology
dc.relation.ispartofAmerican Journal of Clinical Pathology
dc.relation.ispartofjcr2.413
dc.relation.ispartofsjr1,002
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectKaposi sarcomapt
dc.subjectKaposi sarcoma-associated herpesviruspt
dc.subjectKSHVpt
dc.subjecthuman herpesvirus 8pt
dc.subjectHHV-8pt
dc.subjectlatency-associated nuclear antigen 1pt
dc.subjectcleaved caspase-3pt
dc.subjecthuman bcl-2pt
dc.titleHuman bcl-2 expression, cleaved caspase-3, and KSHV LANA-1 in Kaposi sarcoma lesionsen
dc.typeArtigo
dcterms.licensehttp://ajcp.ascpjournals.org/site/misc/ifora.xhtml
dcterms.rightsHolderAmer Soc Clinical Pathology
unesp.author.lattes5240998569868081
unesp.author.orcid0000-0001-5562-9648[4]
unesp.author.orcid0000-0002-0768-8421[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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