Titanium biogenic nanoparticles to help the growth of Trichoderma harzianum to be used in biological control

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dc.contributor.authorPasquoto-Stigliani, Tatiane
dc.contributor.authorGuilger-Casagrande, Mariana [UNESP]
dc.contributor.authorCampos, Estefânia V. R. [UNESP]
dc.contributor.authorGermano-Costa, Tais
dc.contributor.authorBilesky-José, Natalia
dc.contributor.authorMigliorini, Bianca B.
dc.contributor.authorFeitosa, Leandro O.
dc.contributor.authorSousa, Bruno T.
dc.contributor.authorde Oliveira, Halley C.
dc.contributor.authorFraceto, Leonardo F. [UNESP]
dc.contributor.authorLima, Renata
dc.contributor.institutionUniversity of Sorocaba (UNISO)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Londrina (UEL)
dc.date.accessioned2023-07-29T13:56:09Z
dc.date.available2023-07-29T13:56:09Z
dc.date.issued2023-12-01
dc.description.abstractBackground: The biogenic synthesis of metallic nanoparticles is a green alternative that reduces the toxicity of this nanomaterials and may enable a synergy between the metallic core and the biomolecules employed in the process enhancing biological activity. The aim of this study was to synthesize biogenic titanium nanoparticles using the filtrate of the fungus Trichoderma harzianum as a stabilizing agent, to obtain a potential biological activity against phytopathogens and mainly stimulate the growth of T. harzianum, enhancing its efficacy for biological control. Results: The synthesis was successful and reproductive structures remained in the suspension, showing faster and larger mycelial growth compared to commercial T. harzianum and filtrate. The nanoparticles with residual T. harzianum growth showed inhibitory potential against Sclerotinia sclerotiorum mycelial growth and the formation of new resistant structures. A great chitinolytic activity of the nanoparticles was observed in comparison with T. harzianum. In regard to toxicity evaluation, an absence of cytotoxicity and a protective effect of the nanoparticles was observed through MTT and Trypan blue assay. No genotoxicity was observed on V79-4 and 3T3 cell lines while HaCat showed higher sensitivity. Microorganisms of agricultural importance were not affected by the exposure to the nanoparticles, however a decrease in the number of nitrogen cycling bacteria was observed. In regard to phytotoxicity, the nanoparticles did not cause morphological and biochemical changes on soybean plants. Conclusion: The production of biogenic nanoparticles was an essential factor in stimulating or maintaining structures that are important for biological control, showing that this may be an essential strategy to stimulate the growth of biocontrol organisms to promote more sustainable agriculture. Graphical Abstract: [Figure not available: see fulltext.].en
dc.description.affiliationLaboratory for Evaluation of the Bioactivity and Toxicology of Nanomaterials University of Sorocaba (UNISO), São Paulo
dc.description.affiliationInstitute of Science and Technology of Sorocaba Laboratory of Environmental Nanotechnology State University of São Paulo (UNESP), São Paulo
dc.description.affiliationDepartament of Animal and Plant Biology University of Londrina (UEL), Paraná
dc.description.affiliationUnespInstitute of Science and Technology of Sorocaba Laboratory of Environmental Nanotechnology State University of São Paulo (UNESP), São Paulo
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: #2016/13692-6
dc.description.sponsorshipIdFAPESP: #2017/13328-5
dc.description.sponsorshipIdFAPESP: #2017/21004-5
dc.identifierhttp://dx.doi.org/10.1186/s12951-023-01918-y
dc.identifier.citationJournal of Nanobiotechnology, v. 21, n. 1, 2023.
dc.identifier.doi10.1186/s12951-023-01918-y
dc.identifier.issn1477-3155
dc.identifier.scopus2-s2.0-85160201851
dc.identifier.urihttp://hdl.handle.net/11449/248876
dc.language.isoeng
dc.relation.ispartofJournal of Nanobiotechnology
dc.sourceScopus
dc.subjectBiological control
dc.subjectGreen nanoparticle route
dc.subjectMetal oxide nanoparticles
dc.subjectNanoparticle biosynthesis
dc.subjectPhytopathogen
dc.subjectSclerotinia sclerotiorum
dc.titleTitanium biogenic nanoparticles to help the growth of Trichoderma harzianum to be used in biological controlen
dc.typeArtigo

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Artigos - Engenharia Ambiental - Sorocaba