Evaluation of glucose biosensors based on Prussian Blue and lyophilised, crystalline and cross-linked glucose oxidases (CLEC (R))

dc.contributor.authorMattos, Ivanildo Luiz de [UNESP]
dc.contributor.authorLukachova, Lilia V.
dc.contributor.authorGorton, Lo
dc.contributor.authorLaurell, Thomas
dc.contributor.authorKaryakin, Arkady A.
dc.contributor.institutionUniversity of Lund
dc.contributor.institutionMoscow State University (M.V.L.)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:21:23Z
dc.date.available2014-05-20T15:21:23Z
dc.date.issued2001-06-21
dc.description.abstractGlucose biosensors based on lyophilised, crystalline and cross-linked glucose oxidase (GOx, CLEC(R)) and commercially available lyophilised GOx immobilised on top of glassy carbon electrodes modified with electrodeposited Prussian Blue are critically compared. Two procedures were carried out for preparing the biosensors: (1) deposition of one layer of adsorbed GOx dissolved in an aqueous solution followed by deposition of two layers of low molecular weight Nafion(R) dissolved in 90% ethanol, and (2) deposition of two layers of a mixture of GOx with Nafion dissolved in 90% ethanol. The performance of the biosensors was evaluated in terms of linear response range for hydrogen peroxide and glucose, detection limit, and susceptibility to some common interfering species (ascorbic acid, acetaminophen and uric acid). The operational stability of the biosensors was evaluated by applying a steady potential of -50 mV versus Ag/AgCl to the glucose biosensor and injecting standard solutions of hydrogen peroxide and glucose (50 muM and 1.0 mM, respectively, in phosphate buffer) for at least 5 h in a flow-injection system. Scanning electron microscopy was used for visualisation of the Prussian Blue redox catalyst and in the presence of the different GOx preparations on the electrode surface. (C) 2001 Elsevier B.V. B.V. All rights reserved.en
dc.description.affiliationDepartment of Analytical Chemistry University of Lund, Lund
dc.description.affiliationDepartment of Chemistry M.V.L. Moscow State University, Moscow
dc.description.affiliationDepartment of Electrical Measurements University of Lund, Lund
dc.description.affiliationDepartamento de Química Faculdade de Ciências UNESP, P.O. Box 473, 17033-360, Bauru SP
dc.description.affiliationUnespDepartamento de Química Faculdade de Ciências UNESP, P.O. Box 473, 17033-360, Bauru SP
dc.format.extent963-974
dc.identifierhttp://dx.doi.org/10.1016/S0039-9140(01)00367-8
dc.identifier.citationTalanta. Amsterdam: Elsevier B.V., v. 54, n. 5, p. 963-974, 2001.
dc.identifier.doi10.1016/S0039-9140(01)00367-8
dc.identifier.issn0039-9140
dc.identifier.scopus2-s2.0-0035927844
dc.identifier.urihttp://hdl.handle.net/11449/130562
dc.identifier.wosWOS:000169852200020
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTalanta
dc.relation.ispartofjcr4.244
dc.relation.ispartofsjr1,186
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCross-linked enzyme crystals
dc.subjectFlow injection analysis
dc.subjectHydrogen peroxide
dc.subjectPrussian Blue
dc.subjectDeposition
dc.subjectElectrodes
dc.subjectEnzymes
dc.subjectRedox reactions
dc.subjectScanning electron microscopy
dc.subjectElectrode surface
dc.subjectGlucose sensors
dc.subjectAlcohol
dc.subjectAscorbic acid
dc.subjectFerric ferrocyanide
dc.subjectGlucose
dc.subjectGlucose oxidase
dc.subjectParacetamol
dc.subjectPerfluoro compound
dc.subjectUric acid
dc.subjectAdsorption
dc.subjectBiosensor
dc.subjectChemical analysis
dc.subjectElectrode
dc.subjectEnzymatic assay
dc.subjectFlow injection analysis
dc.subjectScanning electron microscopy
dc.titleEvaluation of glucose biosensors based on Prussian Blue and lyophilised, crystalline and cross-linked glucose oxidases (CLEC (R))en
dc.typeArtigo
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências, Baurupt

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