Electrochemical measurements of oligonucleotides in the presence of chromosomal DNA using membrane-covered carbon electrodes

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dc.contributor.authorWang, J.
dc.contributor.authorCai, X. H.
dc.contributor.authorFernandes, JR
dc.contributor.authorGrant, D. H.
dc.contributor.authorOzsoz, M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMT ALLISON UNIV
dc.contributor.institutionEGE UNIV
dc.date.accessioned2014-05-20T15:29:34Z
dc.date.available2014-05-20T15:29:34Z
dc.date.issued1997-10-01
dc.description.abstractSubstantial improvements in the selectivity of electrochemical measurements of trace nucleic adds are obtained by using membrane-covered carbon disk electrodes. Access to the electrode surface can be manipulated via a judicious choice of the membrane molecular weight cutoff (MWCO). The resulting separation step, performed in situ at the electrode surface, adds a new dimension of selectivity based on molecular size to electroanalysis of nucleic acids, Transport properties are evaluated with respect to the oligonucleotide length and membrane MWCO. A highly selective response is observed for synthetic oligonucleotides in the presence of otherwise interfering chromosomal DNAs. Discrimination among oligonucleotides of different lengths is also possible, Short accumulation periods (1-5 min) are sufficient for convenient measurements of low milligram per liter concentrations.en
dc.description.affiliationUNIV ESTADUAL PAULISTA,DEPT QUIM,BAURU,SP,BRAZIL
dc.description.affiliationMT ALLISON UNIV,DEPT CHEM,SACKVILLE,NB E0A 3C0,CANADA
dc.description.affiliationEGE UNIV,FAC PHARM,TR-35100 IZMIR,TURKEY
dc.description.affiliationUnespUNIV ESTADUAL PAULISTA,DEPT QUIM,BAURU,SP,BRAZIL
dc.format.extent4056-4059
dc.identifierhttp://dx.doi.org/10.1021/ac9704319
dc.identifier.citationAnalytical Chemistry. Washington: Amer Chemical Soc, v. 69, n. 19, p. 4056-4059, 1997.
dc.identifier.doi10.1021/ac9704319
dc.identifier.issn0003-2700
dc.identifier.lattes8540599256820672
dc.identifier.urihttp://hdl.handle.net/11449/39131
dc.identifier.wosWOS:A1997XX93300033
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofAnalytical Chemistry
dc.relation.ispartofjcr6.042
dc.relation.ispartofsjr2,362
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleElectrochemical measurements of oligonucleotides in the presence of chromosomal DNA using membrane-covered carbon electrodesen
dc.typeArtigo
dcterms.licensehttp://pubs.acs.org/paragonplus/copyright/jpa_form_a.pdf
dcterms.rightsHolderAmer Chemical Soc
unesp.author.lattes8540599256820672
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências, Baurupt
unesp.departmentQuímica - FCpt

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