In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeys

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dc.contributor.authorAltarugio, R. [UNESP]
dc.contributor.authorVellano, I. H.B. [UNESP]
dc.contributor.authorMoraes, A. C.I. [UNESP]
dc.contributor.authorMilbradt, E. L. [UNESP]
dc.contributor.authorFilho, R.L. Andreatti [UNESP]
dc.contributor.authorGuimarães-Okamoto, P. T.C. [UNESP]
dc.contributor.authorPadovani, C. R. [UNESP]
dc.contributor.authorOkamoto, A. S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:18:25Z
dc.date.available2018-12-11T17:18:25Z
dc.date.issued2018-03-01
dc.description.abstractThe health of poultry is closely related to how the animals are raised, and the gut microbiota plays a key role in the fulfillment of their productive potential. Lactobacillus spp. are bacteria present in the natural microbiota of poultry that, when employed as probiotic agents, should present several characteristics. This study aims at performing the in vitro probiotic isolation and characterization of Lactobacillus spp. Isolates were obtained from ceca content of healthy turkeys through the isolation and identification of morphological, molecular, and physiologic characteristics. They were identified through Gram staining, catalase and hydrogen peroxide production tests, gas production tests during glucose fermentation, and hydrogen sulfide production during the triple sugar iron test. The samples were identified molecularly through polymerase chain reaction tests and were subjected to genetic sequencing. The assessment of the probiotic potential was conducted through artificial gastric juice tolerance and bile salt tolerance tests. A hydrophobicity test was used as an indirect method of assessing an isolate's probable ability to adhere to the intestinal mucosa. In addition, other analyses were performed, such as multiplication potential tests, Salmonella Heildelberg antagonism assays, hydrogen peroxide production tests, and antibiograms, as well as an assessment of the genes responsible for resistance to antimicrobial agents in Integron C. In conclusion, we identified, through morphologic, physiologic, pathogen antagonism, and antimicrobial resistance tests, 11 strains of Lactobacillus spp. belonging to species L. reuteri (9), L. johnsonii (1), and L. frumenti (1) that showed potential as probiotic candidates for in vivo application.en
dc.description.affiliationVeterinary Clinic Department FMVZ UNESP Botucatu - SP
dc.description.affiliationBiostatistic Department IBB UNESP Botucatu - SP
dc.description.affiliationUnespVeterinary Clinic Department FMVZ UNESP Botucatu - SP
dc.description.affiliationUnespBiostatistic Department IBB UNESP Botucatu - SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent81-91
dc.identifierhttp://dx.doi.org/10.3382/japr/pfx045
dc.identifier.citationJournal of Applied Poultry Research, v. 27, n. 1, p. 81-91, 2018.
dc.identifier.doi10.3382/japr/pfx045
dc.identifier.file2-s2.0-85043229989.pdf
dc.identifier.issn1537-0437
dc.identifier.issn1056-6171
dc.identifier.lattes4703983380224373
dc.identifier.lattes5085484980214125
dc.identifier.lattes8502462873517464
dc.identifier.orcid0000-0002-0355-9841
dc.identifier.orcid0000-0003-1944-2426
dc.identifier.orcid0000-0003-4939-8024
dc.identifier.scopus2-s2.0-85043229989
dc.identifier.urihttp://hdl.handle.net/11449/175985
dc.language.isoeng
dc.relation.ispartofJournal of Applied Poultry Research
dc.relation.ispartofsjr0,585
dc.relation.ispartofsjr0,585
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectMicrobiota
dc.subjectPathogen
dc.subjectPoultry
dc.subjectProbiotic
dc.subjectResistance
dc.titleIn vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeysen
dc.typeArtigo
unesp.author.lattes4703983380224373[8]
unesp.author.lattes5085484980214125[6]
unesp.author.lattes8727897080522289[7]
unesp.author.lattes8502462873517464[5]
unesp.author.orcid0000-0002-0355-9841[8]
unesp.author.orcid0000-0003-1944-2426[6]
unesp.author.orcid0000-0002-7719-9682[7]
unesp.author.orcid0000-0003-4939-8024[5]

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Artigos - Clínica Veterinária - FMVZ
Artigos - Bioestatística - IBB