Role of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interaction

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dc.contributor.authorMoraes, Karen C. M. [UNESP]
dc.contributor.authorDiniz, Livia F.
dc.contributor.authorBahia, Maria Terezinha
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Ouro Preto (UFOP)
dc.date.accessioned2015-10-21T13:12:12Z
dc.date.available2015-10-21T13:12:12Z
dc.date.issued2015-04-01
dc.description.abstractChagas disease, caused by the intracellular protozoan Trypanosoma cruzi, is a serious health problem in Latin America. During this parasitic infection, the heart is one of the major organs affected. The pathogenesis of tissue remodelling, particularly regarding cardiomyocyte behaviour after parasite infection and the molecular mechanisms that occur immediately following parasite entry into host cells are not yet completely understood. When cells are infected with T. cruzi, they develop an inflammatory response, in which cyclooxygenase-2 (COX-2) catalyses rate-limiting steps in the arachidonic acid pathway. However, how the parasite interaction modulates COX-2 activity is poorly understood. In this study, the H9c2 cell line was used as our model and we investigated cellular and biochemical aspects during the initial 48 h of parasitic infection. Oscillatory activity of COX-2 was observed, which correlated with the control of the pro-inflammatory environment in infected cells. Interestingly, subcellular trafficking was also verified, correlated with the control of Cox-2 mRNA or the activated COX-2 protein in cells, which is directly connected with the assemble of stress granules structures. Our collective findings suggest that in the very early stage of the T. cruzi-host cell interaction, the parasite is able to modulate the cellular metabolism in order to survives.en
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Biol, Lab Biol Mol, Rio Claro, SP, Brazil
dc.description.affiliationUniv Fed Ouro Preto, Dept Ciencias Biol, Lab Doenca Chagas, Nucleo Pesquisa Ciencias Biol, Ouro Preto, MG, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Biol, Lab Biol Mol, Rio Claro, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipPROPE-UFOP
dc.description.sponsorshipIdFAPEMIG: CBB-APQ-02351-10
dc.description.sponsorshipIdFAPEMIG: CBB-APQ-01700-11
dc.description.sponsorshipIdCNPq: 475586/2009-3
dc.format.extent181-191
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000200181&lng=en&nrm=iso&tlng=en
dc.identifier.citationMemorias do Instituto Oswaldo Cruz, v. 110, n. 2, p. 181-191, 2015.
dc.identifier.doi10.1590/0074-02760140311
dc.identifier.fileS0074-02762015000200181.pdf
dc.identifier.issn0074-0276
dc.identifier.scieloS0074-02762015000200181
dc.identifier.urihttp://hdl.handle.net/11449/128678
dc.identifier.wosWOS:000354066400004
dc.language.isoeng
dc.publisherFundaco Oswaldo Cruz
dc.relation.ispartofMemorias do Instituto Oswaldo Cruz
dc.relation.ispartofjcr2.833
dc.relation.ispartofsjr1,172
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCardiac cellsen
dc.subjectChagas diseaseen
dc.subjectEnzyme activityen
dc.subjectFluorescence microscopyen
dc.subjectPro-inflammatory processen
dc.subjectSubcellular traffickingen
dc.titleRole of cyclooxygenase-2 in Trypanosoma cruzi survival in the early stages of parasite host-cell interactionen
dc.typeArtigo
dcterms.rightsHolderFundaco Oswaldo Cruz
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Artigos - Biologia - IBRC