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Efficient flotation of yeast cells grown in batch culture

dc.contributor.authorPalmieri, M. C.
dc.contributor.authorGreenhalf, W.
dc.contributor.authorLaluce, Cecília [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:27:58Z
dc.date.available2014-05-20T15:27:58Z
dc.date.issued1996-05-05
dc.description.abstractA fast flotation assay was used to select new floating yeast strains. The flotation ability did not seem to be directly correlated to total extracellular protein concentration of the culture. However, the hydrophobicity of the cell was definitely correlated to the flotation capacity. The Saccharomyces strains (FLT strains) were highly hydrophobic and showed an excellent flotation performance in batch cultures without additives (flotation agents) and with no need for a special flotation chamber or flotation column. A stable and well-organized structure was evident in the dried foam as shown by scanning electron microscopy which revealed its unique structure showing mummified cells (dehydrated) attached to each other. The attachment among the cells and the high protein concentration of the foams indicated that proteins might be involved in the foam formation. The floating strains (strains FLT) which were not flocculent and showed no tendency to aggregate, were capable of growing and producing ethanol in a synthetic medium containing high glucose concentration as a carbon source. The phenomenon responsible for flotation seems to be quite different from the flocculation phenomenon. (C) 1996 John Wiley & Sons, Inc.en
dc.description.affiliationUNESP,DEPT BIOQUIM,INST QUIM,BR-14800900 ARARAQUARA,SP,BRAZIL
dc.description.affiliationUnespUNESP,DEPT BIOQUIM,INST QUIM,BR-14800900 ARARAQUARA,SP,BRAZIL
dc.format.extent248-256
dc.identifierhttp://dx.doi.org/10.1002/(SICI)1097-0290(19960505)50:3<248
dc.identifier.citationBiotechnology and Bioengineering. New York: John Wiley & Sons Inc., v. 50, n. 3, p. 248-256, 1996.
dc.identifier.doi10.1002/(SICI)1097-0290(19960505)50:3<248
dc.identifier.issn0006-3592
dc.identifier.urihttp://hdl.handle.net/11449/37887
dc.identifier.wosWOS:A1996UE81200003
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofBiotechnology and Bioengineering
dc.relation.ispartofjcr3.952
dc.relation.ispartofsjr1,372
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectyeastpt
dc.subjectSaccharomycespt
dc.subjectflotationpt
dc.subjectbatch culturept
dc.titleEfficient flotation of yeast cells grown in batch cultureen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes6423374942748412[3]
unesp.author.orcid0000-0002-2462-7997[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.departmentBioquímica e Tecnologia - IQpt

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