Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom

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dc.contributor.authorVinhote, J. F.C.
dc.contributor.authorTorres, A. F.C.
dc.contributor.authorDantas, R. T.
dc.contributor.authorPraciano, T. P.
dc.contributor.authorMenezes, R. R.P.P.B.
dc.contributor.authorSousa, D. F.
dc.contributor.authorBrito, T. S.
dc.contributor.authorLima, F. J.B.
dc.contributor.authorToyama, M. H. [UNESP]
dc.contributor.authorMagalhães, P. J.
dc.contributor.authorMonteiro, H. A.S.
dc.contributor.authorMartins-Nunes, A. M.C.
dc.contributor.institutionFederal University of Ceará
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T22:37:26Z
dc.date.available2022-04-28T22:37:26Z
dc.date.issued2011-06-13
dc.description.abstractIn the present study, the effects of Polybia paulista venom (PPV) on renal and vascular tissues were investigated. Isolated kidneys perfused with PPV (1 and 3 μg/mL) had increased perfusion pressure, renal vascular resistance, urinary flow, and glomerular filtration rate; and reduced sodium tubular transport. Histological evaluation demonstrated deposits of proteins in Bowman's space and tubular lumen, and focal areas of necrosis. The venom promoted a cytotoxic effect on Madin-Darby canine kidney (MDCK) cells. A significant increase in lactic dehydrogenase levels was observed in response to venom exposure. In isolated mesenteric vascular beds, pressure and vascular resistance augmented in a dose-dependent manner. PPV increased the contractility of aortic rings maintained under basal tension. This contractile response was inhibited when preparations were maintained in Ca2+-free medium. Likewise, verapamil, a voltage-gated calcium channel blocker, also inhibited the contractile response. In this study, phentolamine, a blocker of α-adrenergic receptor blocker, significantly reduced the contractile effect of PPV in the aortic ring. In conclusion, PPV produced nephrotoxicity, which suggests a direct effect on necrotic cellular death in renal tubule cells. The vascular contractile effect of PPV appears to involve calcium influx through voltage-gated calcium channels via adrenergic regulation. © CEVAP 2011.en
dc.description.affiliationDepartment of Physiology and Pharmacology Federal University of Ceará, Fortaleza
dc.description.affiliationDepartment of Clinical and Toxicological Analyses Federal University of Ceará, Fortaleza, Ceará State
dc.description.affiliationSão Paulo Experimental Coast Campus São Paulo State University (UNESP - Univ Estadual Paulista), São Vicente, São Paulo State
dc.description.affiliationUnespSão Paulo Experimental Coast Campus São Paulo State University (UNESP - Univ Estadual Paulista), São Vicente, São Paulo State
dc.format.extent199-208
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992011000200011
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases, v. 17, n. 2, p. 199-208, 2011.
dc.identifier.doi10.1590/S1678-91992011000200011
dc.identifier.issn1678-9199
dc.identifier.scopus2-s2.0-79958155374
dc.identifier.urihttp://hdl.handle.net/11449/226358
dc.language.isoeng
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.sourceScopus
dc.subjectAorta
dc.subjectKidney
dc.subjectMDCK
dc.subjectPolybia paulista
dc.subjectVenom
dc.titleRenal- and calcium-dependent vascular effects of Polybia paulista wasp venomen
dc.typeArtigo

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