Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

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dc.contributor.authorAquino, Lúcia Padilha Cury Thomaz [UNESP]
dc.contributor.authorMachado, Rosangela Zacarias [UNESP]
dc.contributor.authorLemos, Karen Regina [UNESP]
dc.contributor.authorMarques, Luiz Carlos [UNESP]
dc.contributor.authorGarcia, Marcos Valerio [UNESP]
dc.contributor.authorBorges, Gustavo Puia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:48:16Z
dc.date.available2022-04-29T08:48:16Z
dc.date.issued2010-01-01
dc.description.abstractThe present research investigated the presence of T. evansi antibodies in animals from the subregion of Nhecolandia, in the Pantanal Sul-mato-grossense, by means of an enzyme linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT), and the pattern of polypeptide recognition by sera from experimentally and naturally infected hosts using Western blotting. Serum samples were obtained from bovines (n = 102), horses (n = 98), and dogs (n = 55), and from 32 free-ranging coatis (Nasua nasua). None of the bovines were found positive, while sera from 16 dogs (29%) and 23 horses (23.4%) were positive by ELISA. Sera from 8 coatis (25%) were found positive using IFAT. Western blotting revealed major polypeptides of T. evansi with molecular weight ranging from 74 to 38 kDa. The polypeptides of 66, 48-46, and 38 kDa were identified by sera from experimentally infected bovines, donkeys, dogs, and coatis. The 48-46 and 38 kDa bands were mainly recognized in chronic phase of infection. The antigen with apparent molecular weight of 66 kDa, revealed by antibodies from all experimental animals, was also recognized in sera of horses and dogs from the Pantanal. The 48-46 kDa polypeptide was identified by antibodies from all naturally infected animals and must be further evaluated for use in specific diagnosis of T. evansi infection.en
dc.description.affiliationFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista - UNESP
dc.description.affiliationUnespFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista - UNESP
dc.format.extent38-44
dc.identifier.citationRevista Brasileira de Parasitologia Veterinaria, v. 19, n. 2, p. 38-44, 2010.
dc.identifier.issn0103-846X
dc.identifier.scopus2-s2.0-77954259874
dc.identifier.urihttp://hdl.handle.net/11449/231937
dc.language.isopor
dc.relation.ispartofRevista Brasileira de Parasitologia Veterinaria
dc.sourceScopus
dc.subjectAntigenic characterization
dc.subjectELISA-test
dc.subjectIFAT
dc.subjectPantanal mato-grossense
dc.subjectTrypanosoma evansi
dc.titleAntigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatisen
dc.titleCaracterização antigênica do Trypanosoma evansi usando soros de bovinos, equinos, cães e quatis experimentalmente e naturalmente infectadospt
dc.typeArtigo
unesp.departmentClínica e Cirurgia Veterinária - FCAVpt
unesp.departmentPatologia Veterinária - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias