Alternative methodologies for the determination of aldehydes by capillary electrophoresis
| dc.contributor.author | Pereira, Elisabete Alves | |
| dc.contributor.author | Tavares, Marina Franco Maggi | |
| dc.contributor.author | Cardoso, Arnaldo Alves [UNESP] | |
| dc.contributor.institution | Universidade de São Paulo (USP) | |
| dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
| dc.date.accessioned | 2022-04-29T08:46:49Z | |
| dc.date.available | 2022-04-29T08:46:49Z | |
| dc.date.issued | 1999-01-01 | |
| dc.description.abstract | This paper describes 2 alternative methodologies for the determination of selected aldehydes (formaldehyde, acetaldehyde, propionaldehyde, acrolein, and benzaldehyde) by capillary electrophoresis (CE). The first approach is based on the formation of aldehyde-bisulfite adducts and employs free solution CE with reversed electroosmotic flow and indirect detection, using 10 mmol/L 3,5-dinitrobenzoic acid (pH 4.5) containing 0.2 mmol/L cetyltrimethylammonium bromide as the electrolyte. This novel methodology showed a fairly good sensitivity to concentration, with detection limits with respect to a single aldehyde on the order of 10-40 μg/L, a reasonable analysis time (separation was achieved in <8 min), and no need for sample manipulation. A second approach was proposed in which 2,4-dinitrophenylhydrazine derivatives of the aldehydes were detected in a micellar electrolyte medium (20 mmol/L borate buffer containing 50 mmol/L sodium dodecyl sulfate and 15 mmol/L β-cyclodextrin). This latter methodology included a laborious sample preconcentration step and showed much poorer sensitivity (0.5-2 mg/L detection limit, with respect to a single aldehyde), despite the use of sodium chloride to promote sample stacking. Both methodologies proved adequate to evaluate aldehyde levels in vehicular emissions. Samples from the tailpipe exhaust of a passenger car vehicle without a catalytic converter and operated with an ethanol-based fuel were collected and analyzed; the results showed high levels of formaldehyde and acetaldehyde (0.41-6.1 ppm, v/v). The concentrations estimated by the 2 methodologies, which were not in good agreement, suggest the possibility of striking differences in sample collection efficiency, which was not the concern of this work. | en |
| dc.description.affiliation | Universidade de São Paulo Instituto de Química, C.P. 26077, 05599-970, São Paulo, SP | |
| dc.description.affiliation | Universidade Estadual Paulista Instituto de Química, C.P. 355, 14800-910, Araraquara, SP | |
| dc.description.affiliationUnesp | Universidade Estadual Paulista Instituto de Química, C.P. 355, 14800-910, Araraquara, SP | |
| dc.format.extent | 1562-1570 | |
| dc.identifier | http://dx.doi.org/10.1093/jaoac/82.6.1562 | |
| dc.identifier.citation | Journal of AOAC International, v. 82, n. 6, p. 1562-1570, 1999. | |
| dc.identifier.doi | 10.1093/jaoac/82.6.1562 | |
| dc.identifier.issn | 1060-3271 | |
| dc.identifier.scopus | 2-s2.0-0001226694 | |
| dc.identifier.uri | http://hdl.handle.net/11449/231655 | |
| dc.language.iso | eng | |
| dc.relation.ispartof | Journal of AOAC International | |
| dc.source | Scopus | |
| dc.title | Alternative methodologies for the determination of aldehydes by capillary electrophoresis | en |
| dc.type | Artigo |