Synthesis of the peptide Ac-Wahx-KTTKS and evaluation of the ability to induce in vitro collagen synthesis

Página do item simplificado
dc.contributor.authorDanila, A. S. Guglielmi [UNESP]
dc.contributor.authorAriane, M. Martinelli [UNESP]
dc.contributor.authorNathália, C. Rissi [UNESP]
dc.contributor.authorEduardo, M. Cilli [UNESP]
dc.contributor.authorChristiane, P. Soares [UNESP]
dc.contributor.authorLeila, A. Chiavacci [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T16:42:45Z
dc.date.available2018-12-11T16:42:45Z
dc.date.issued2016-06-01
dc.description.abstractIn this work, we report the synthesis of a peptide analogue of the KTTKS, termed Ac-Wahx-KTTKS and evaluate its cytotoxicity and role in biosynthesis of collagen for future application in skin aging. The peptide was obtained with purity higher than 97.5%. In the cytotoxicity assay, we observed non-toxic effects for Ac-WAhx-KTTKS at concentrations below 600 ôM for HaCaT and 500 ôM for HepG2 cells, respectively. After 24 and 48 h it was possible to observe significant changes in collagen synthesis in the groups treated with various concentrations of the peptide. In conclusion, the Ac-Wahx-KTTKS peptide increased collagen synthesis in fibroblasts by 80% and it is a promising candidate for improving skin aging.en
dc.description.affiliationFaculdade de Ciências Farmacêuticas UNESP- Univ Estadual Paulista, Araraquara-Jaú, Km 1
dc.description.affiliationInstituto de Química UNESP-Univ Estadual Paulista, Prof. Francisco Degni Street, 55
dc.description.affiliationUnespFaculdade de Ciências Farmacêuticas UNESP- Univ Estadual Paulista, Araraquara-Jaú, Km 1
dc.description.affiliationUnespInstituto de Química UNESP-Univ Estadual Paulista, Prof. Francisco Degni Street, 55
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent544-547
dc.identifier.citationProtein and Peptide Letters, v. 23, n. 6, p. 544-547, 2016.
dc.identifier.issn1875-5305
dc.identifier.issn0929-8665
dc.identifier.scopus2-s2.0-84974735668
dc.identifier.urihttp://hdl.handle.net/11449/168728
dc.language.isoeng
dc.relation.ispartofProtein and Peptide Letters
dc.relation.ispartofsjr0,429
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAc-WAhx-KTTKS
dc.subjectAntiaging
dc.subjectCollagen biosynthesis
dc.subjectCytotoxicity
dc.subjectPeptide synthesis
dc.subjectSkin functions.
dc.titleSynthesis of the peptide Ac-Wahx-KTTKS and evaluation of the ability to induce in vitro collagen synthesisen
dc.typeArtigo

Arquivos

Coleções

Artigos