Anti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided study

dc.contributor.authorSouza-Moreira, Tatiana M. [UNESP]
dc.contributor.authorSeveri, Juliana A. [UNESP]
dc.contributor.authorLee, Keunsook
dc.contributor.authorPreechasuth, Kanya
dc.contributor.authorSantos, Emerson [UNESP]
dc.contributor.authorGow, Neil A.R.
dc.contributor.authorMunro, Carol A.
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorPietro, Rosemeire C.L.R. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Aberdeen
dc.contributor.institutionAlto Universitário
dc.date.accessioned2014-05-27T11:29:53Z
dc.date.available2014-05-27T11:29:53Z
dc.date.issued2013-07-01
dc.description.abstractIn addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level. © 2013 by the authors.en
dc.description.affiliationDepartment of Drugs and Medicines School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista, Rodovia Araraquara-Jau, km 1, Araraquara, 14801-902, São Paulo
dc.description.affiliationDepartment of Microbiology Institute of Medical Sciences University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD
dc.description.affiliationDepartment of Organic Chemistry Institute of Chemistry UNESP-Univ Estadual Paulista, R. Francisco Degni s/n, Araraquara, 14800-900, São Paulo
dc.description.affiliationDepartment of Pharmacy and Nutrition Centro de Ciências Agrárias Alto Universitário, Guararema, 29500-000, Alegre, ES
dc.description.affiliationUnespDepartment of Drugs and Medicines School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista, Rodovia Araraquara-Jau, km 1, Araraquara, 14801-902, São Paulo
dc.description.affiliationUnespDepartment of Organic Chemistry Institute of Chemistry UNESP-Univ Estadual Paulista, R. Francisco Degni s/n, Araraquara, 14800-900, São Paulo
dc.format.extent8095-8108
dc.identifierhttp://dx.doi.org/10.3390/molecules18078095
dc.identifier.citationMolecules, v. 18, n. 7, p. 8095-8108, 2013.
dc.identifier.doi10.3390/molecules18078095
dc.identifier.file2-s2.0-84880795871.pdf
dc.identifier.issn1420-3049
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.scopus2-s2.0-84880795871
dc.identifier.urihttp://hdl.handle.net/11449/75864
dc.identifier.wosWOS:000330300900048
dc.language.isoeng
dc.relation.ispartofMolecules
dc.relation.ispartofjcr3.098
dc.relation.ispartofsjr0,855
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAntifungal activity
dc.subjectCandida
dc.subjectCell wall
dc.subjectEllagitannin
dc.subjectPlinia cauliflora (Myrtaceae)
dc.titleAnti-Candida targets and cytotoxicity of casuarinin isolated from Plinia cauliflora leaves in a bioactivity-guided studyen
dc.typeArtigo
dcterms.licensehttp://www.mdpi.com/about/openaccess
unesp.author.orcid0000-0003-3032-2556[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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