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Stabilization and application of spray-dried tannase from Aspergillus fumigatus CAS21 in the presence of different carriers

dc.contributor.authorCavalcanti, Rayza Morganna Farias [UNESP]
dc.contributor.authorMartinez, Marcelo Luís Lombardi
dc.contributor.authorOliveira, Wanderley Pereira
dc.contributor.authorGuimarães, Luís Henrique Souza
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2020-12-12T01:18:56Z
dc.date.available2020-12-12T01:18:56Z
dc.date.issued2020-04-01
dc.description.abstractThe Aspergillus fumigatus CAS21 tannase was spray dried with β-cyclodextrin, Capsul® starch, soybean meal, lactose, and maltodextrin as adjuvants. The moisture content and water activity of the products ranged from 5.6 to 11.5% and from 0.249 to 0.448, respectively. The maximal tannase activity was achieved at 40–60 ºC and pH 5.0–6.0 for the powders containing β-cyclodextrin and Capsul® starch, which was stable at 40 ºC and 40–60 ºC for 120 min, respectively. For all the dried products, tannase retained its activity of over 80% for 120 min at pH 5.0 and 6.0. Salts and solvents influenced the activity of the spray-dried tannase. The activity of the spray-dried tannase was maintained when preserved for 1 year at 4 ºC and 28 ºC. Spray-dried tannase reduced the content of tannins and polyphenolic compounds of leather effluent and sorghum flour and catalyzed the transesterification reaction. The spray drying process stabilized the tannase activity, highlighting the potential of dried products for biotechnological applications.en
dc.description.affiliationInstituto de Química de Araraquara – UNESP, Avenida Professor Mário Degni s/nº, Quitandinha
dc.description.affiliationFaculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo USP, Av. do Café s/n
dc.description.affiliationFaculdade de Filosofia Ciências e Letras de Ribeirão Preto – USP, Avenida Bandeirantes 3900
dc.description.affiliationUnespInstituto de Química de Araraquara – UNESP, Avenida Professor Mário Degni s/nº, Quitandinha
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 142389/2017-0
dc.description.sponsorshipIdFAPESP: 2016/11311-5
dc.identifierhttp://dx.doi.org/10.1007/s13205-020-2164-z
dc.identifier.citation3 Biotech, v. 10, n. 4, 2020.
dc.identifier.doi10.1007/s13205-020-2164-z
dc.identifier.issn2190-5738
dc.identifier.issn2190-572X
dc.identifier.scopus2-s2.0-85082322140
dc.identifier.urihttp://hdl.handle.net/11449/198666
dc.language.isoeng
dc.relation.ispartof3 Biotech
dc.sourceScopus
dc.subjectAspergillus
dc.subjectEnzyme stabilization
dc.subjectSpray drying
dc.subjectTannin
dc.subjectTannin acyl hydrolase
dc.titleStabilization and application of spray-dried tannase from Aspergillus fumigatus CAS21 in the presence of different carriersen
dc.typeArtigo
unesp.author.orcid0000-0002-2921-3929[4]

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