Cryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectant

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dc.contributor.authorColauto, Nelson Barros
dc.contributor.authorda Eira, Augusto Ferreira [UNESP]
dc.contributor.authorLinde, Giani Andrea
dc.contributor.institutionUniv Paranaense UNIPAR
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:30:50Z
dc.date.available2014-05-20T15:30:50Z
dc.date.issued2012-11-01
dc.description.abstractThe preservation of Agaricus blazei is generally done using successive subcultivations that are laborious and are subject to contaminations or genetic degenerations, resulting in loss of biotechnological interest characteristics. An alternative process would be cryopreservation, but there are no reports of methodologies for this basidiomycete in liquid nitrogen. Thus, the objective of this study was to evaluate mycelial viability of A. blazei strains after cryopreservation in liquid nitrogen in order to establish the initial parameters of species preservation. Five strains grown on malt extract agar (MEA) were used. Disks of MEA containing A. blazei mycelium were transferred for screw-cap cryovials containing the cryoprotectant, 10% dimethyl sulfoxide. Then, they were cooled at 8 degrees C for 30 min and kept at -196 degrees C with liquid nitrogen. After 1.5 year of cryopreservation, the cryovials were thawed in water bath at 30 degrees C for 15 min. The disks with mycelia were transferred to MEA culture media without cryoprotectant and kept at 28 degrees C for 30 days. A. blazei strains respond differently to the cryopreservation method at -196 degrees C by varying mycelial viability recovery. Cryopreservation with liquid nitrogen, using dimethyl sulfoxide as cryoprotectant, is not the most appropriate one for A. blazei preservation.en
dc.description.affiliationUniv Paranaense UNIPAR, Lab Biol Mol, Umuarama, PR, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent1034-1037
dc.identifierhttp://www.seer.ufu.br/index.php/biosciencejournal/article/view/14114
dc.identifier.citationBioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 28, n. 6, p. 1034-1037, 2012.
dc.identifier.fileWOS000317382600025.pdf
dc.identifier.issn1516-3725
dc.identifier.urihttp://hdl.handle.net/11449/40140
dc.identifier.wosWOS:000317382600025
dc.language.isoeng
dc.publisherUniversidade Federal de Uberlândia (UFU)
dc.relation.ispartofBioscience Journal
dc.relation.ispartofsjr0,303
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectAgaricus brasiliensisen
dc.subjectAgaricus subrufescensen
dc.subjectThawingen
dc.subjectFreezingen
dc.subjectViabilityen
dc.subjectPreservationen
dc.titleCryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectanten
dc.title.alternativeCriopreservação de Agaricus blazei em nitrogênio líquido usando dmso como crioprotetores
dc.typeArtigo
dcterms.licensehttp://www.seer.ufu.br/index.php/biosciencejournal/about/editorialPolicies#openAccessPolicy
dcterms.rightsHolderUniversidade Federal de Uberlândia (UFU)

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