Nandrolone decanoate causes uterine injury by changing hormone levels and sex steroid receptors in a dose- and time-dependent manner

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dc.contributor.authorSimão, Vinícius Augusto [UNESP]
dc.contributor.authorLupi Júnior, Luiz Antonio [UNESP]
dc.contributor.authorAdan Araujo Leite, Gabriel
dc.contributor.authorCherici Camargo, Isabel Cristina [UNESP]
dc.contributor.authorde Almeida Chuffa, Luiz Gustavo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Santa Catarina (UFSC)
dc.date.accessioned2021-06-25T10:30:18Z
dc.date.available2021-06-25T10:30:18Z
dc.date.issued2021-06-01
dc.description.abstractDifferent doses of nandrolone decanoate (ND) were used to investigate the expression of uterine sex steroid receptors (AR, ER-α, and ER-β) and the levels of serum sex hormones after treatment and recovery periods in adult rats. ND doses of 1.87, 3.75, 7.5, or 15 mg/kg b.w. or mineral oil (control group) were injected subcutaneously for 15 days, and the experimental groups were divided into three periods of evaluation: (a) ND treatment for 15 days, (b) ND treatment followed by 30-day-recovery and (c) ND treatment followed by 60-day-recovery. Estrous cycle was monitored daily. At the end of each experimental period, rats were euthanized for the collection of serum samples and uterine tissues. All animals showed persistent diestrus and only the highest ND dose was capable of inducing persistent diestrus until 60-day-recovery. Immunoexpression of uterine sex steroid receptors varied in a time-dependent manner. While AR expression was increase after treatment period, ER-α and ER-β expressions decreased after 60- and 30-day-recovery, respectively. ND also increased the serum levels of testosterone, 17β-estradiol, and dihydrotestosterone, especially at the highest doses of 7.5 and 15 mg ND/kg until 30 days of recovery. The levels of progesterone were significantly reduced in all ND-treated animals. No significant difference was observed in the levels of follicle-stimulating hormone, whereas the levels of luteinizing hormone varied according to specific dose and period. We conclude that uterine sex steroid receptors and sex hormones are affected by ND administration and these alterations can be only restored following lower doses and long recovery periods.en
dc.description.affiliationDepartment of Biotechnology Faculty of Sciences and Letters of Assis FCL/UNESP
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu IBB/UNESP
dc.description.affiliationDepartment of Cell Biology Embryology and Genetics Biological Sciences Center UFSC
dc.description.affiliationUnespDepartment of Biotechnology Faculty of Sciences and Letters of Assis FCL/UNESP
dc.description.affiliationUnespDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu IBB/UNESP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/01747-0
dc.description.sponsorshipIdFAPESP: 2013/14510-0
dc.format.extent98-108
dc.identifierhttp://dx.doi.org/10.1016/j.reprotox.2021.05.002
dc.identifier.citationReproductive Toxicology, v. 102, p. 98-108.
dc.identifier.doi10.1016/j.reprotox.2021.05.002
dc.identifier.issn1873-1708
dc.identifier.issn0890-6238
dc.identifier.scopus2-s2.0-85105751192
dc.identifier.urihttp://hdl.handle.net/11449/206334
dc.language.isoeng
dc.relation.ispartofReproductive Toxicology
dc.sourceScopus
dc.subjectAnabolic-androgenic steroid
dc.subjectNandrolone decanoate
dc.subjectRats
dc.subjectRecovery period
dc.subjectUterus
dc.titleNandrolone decanoate causes uterine injury by changing hormone levels and sex steroid receptors in a dose- and time-dependent manneren
dc.typeArtigo
unesp.author.orcid0000-0002-3915-2795[1]

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