Soluble factors from biofilm of Candida albicans and Staphylococcus aureus promote cell death and inflammatory response

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dc.contributor.authorDe Carvalho Dias, Kassia [UNESP]
dc.contributor.authorBarbugli, Paula Aboud [UNESP]
dc.contributor.authorDe Patto, Fernanda [UNESP]
dc.contributor.authorLordello, Virginia Barreto [UNESP]
dc.contributor.authorDe Aquino Penteado, Letícia [UNESP]
dc.contributor.authorMedeiros, Alexandra Ivo [UNESP]
dc.contributor.authorVergani, Carlos Eduardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:13:05Z
dc.date.available2018-12-11T17:13:05Z
dc.date.issued2017-06-30
dc.description.abstractBackground: The objective of this study was to better understand the effects of soluble factors from biofilm of single- and mixed-species Candida albicans (C. albicans) and methicillin-sensitive Staphylococcus aureus (MSSA) cultures after 36 h in culture on keratinocytes (NOK-si and HaCaT) and macrophages (J774A.1). Soluble factors from biofilms of C. albicans and MSSA were collected and incubated with keratinocytes and macrophages, which were subsequently evaluated by cell viability assays (MTT). Lactate dehydrogenase (LDH) enzyme release was measured to assess cell membrane damage to keratinocytes. Cells were analysed by brightfield microscopy after 2 and 24 h of exposure to the soluble factors from biofilm. Cell death was detected by labelling apoptotic cells with annexin V and necrotic cells with propidium iodide (PI) and was visualized via fluorescence microscopy. Soluble factors from biofilm were incubated with J774A.1 cells for 24 h; the subsequent production of NO and the cytokines IL-6 and TNF-α was measured by ELISA. Results: The cell viability assays showed that the soluble factors of single-species C. albicans cultures were as toxic as the soluble factors from biofilm of mixed cultures, whereas the soluble factors of MSSA cultures were less toxic than those of C. albicans or mixed cultures. The soluble factors from biofilm of mixed cultures were the most toxic to the NOK-si and HaCaT cells, as confirmed by analyses of PI labelling and cell morphology. Soluble factors from biofilm of single-species MSSA and mixed-species cultures induced the production of IL-6, NO and TNF-α by J744A.1 macrophages. The production of IL-6 and NO induced by the soluble factors from biofilm of mixed cultures was lower than that induced by the soluble factors from biofilm of single-species MSSA cultures, whereas the soluble factors from biofilm of C. albicans cultures induced only low levels of NO. Conclusions: Soluble factors from 36-h-old biofilm of C. albicans and MSSA cultures promoted cell death and inflammatory responses.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics Oral Rehabilitation Program-Araraquara School of Dentistry UNESP-Univ. Estadual Paulista
dc.description.affiliationDepartment of Biological Sciences School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics Oral Rehabilitation Program-Araraquara School of Dentistry UNESP-Univ. Estadual Paulista
dc.description.affiliationUnespDepartment of Biological Sciences School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista
dc.identifierhttp://dx.doi.org/10.1186/s12866-017-1031-5
dc.identifier.citationBMC Microbiology, v. 17, n. 1, 2017.
dc.identifier.doi10.1186/s12866-017-1031-5
dc.identifier.file2-s2.0-85021714088.pdf
dc.identifier.issn1471-2180
dc.identifier.scopus2-s2.0-85021714088
dc.identifier.urihttp://hdl.handle.net/11449/174833
dc.language.isoeng
dc.relation.ispartofBMC Microbiology
dc.relation.ispartofsjr1,242
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiofilm
dc.subjectCandida albicans
dc.subjectCytokines
dc.subjectKeratinocytes
dc.subjectMacrophages
dc.subjectStaphylococcus aureus
dc.titleSoluble factors from biofilm of Candida albicans and Staphylococcus aureus promote cell death and inflammatory responseen
dc.typeArtigo
unesp.author.lattes8756770929017974[6]
unesp.author.lattes3003130522427820[7]
unesp.author.orcid0000-0002-7375-4714[7]
unesp.author.orcid0000-0001-6048-3647[6]

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Artigos - Odontologia Restauradora - FOAR
Artigos - Ciências Biológicas - FCFAR