Artigos - Odontologia Restauradora - FOAR

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  • ItemArtigo
    Etch-and-rinse versus self-etch strategy of a universal adhesive in different application methods at the bonding interface of fiber post cementation
    (2023-01-01) Barros, Antonia Patricia Oliveira ; de Melo Alencar, Cristiane ; Zambon, Matheus ; de Andrade, Marcelo Ferrarezi ; Fernández, Eduardo ; Kuga, Milton Carlos ; Universidade Estadual Paulista (UNESP) ; University Center of the State of Pará ; Universidad Autonoma de Chile
    Objective: To evaluate the effect of etch-and-rinse (ER) versus self-etch (SE) strategies of a universal adhesive applied with a manual brush (MB) or rotary brush (RB) on the bonding interface of fiber post cementation. Methods: Forty bovine incisor roots were prepared and divided into four groups according to the methods of application and strategy of universal adhesive use (MB-ER, RB-ER, MB-SE and RB-SE). After 6 months, specimens from different thirds of the post space were evaluated of push-out strength, analysis of adhesive failure patterns, and extent of tags. Results: The RB-ER and RB-SE groups showed the highest bond strength values in the cervical and middle thirds of the post space. Cohesive adhesive failure had the highest incidence in the different thirds of the post space in the ER strategy, regardless of the adhesive application method. The RB-ER group showed the highest tag extensions. Conclusions: The protocols of universal adhesive application with RB provided the greater bond strength, but only the ER strategy favored the formation of a greater extension of tags at the adhesive interface. Clinical Significance: The application of universal adhesive with RB into the post space increases the strength of the post-cemented fiber bond.
  • ItemArtigo
    Effect of aging and cementation systems on the bond strength to root dentin after fiber post cementation
    (2023-01-01) Nogueira, Caio Henrique de Paula ; Gelio, Mariana Bena ; Besegato, João Felipe ; Ramos, Anna Thereza Peroba Rezende ; Fernández, Eduardo ; Kuga, Milton Carlos ; Saad, José Roberto Cury ; Universidade Estadual Paulista (UNESP) ; University of Chile ; Autonomous University of Chile
    This study evaluated the effect of aging and cementation of fiber posts using glass ionomer and resin cements on push-out bond strength, failure mode, and resin tag formation. One hundred and twenty bovine incisors were used. After post-space preparation, the specimens were randomly allocated into 12 groups (n = 10) according to the cementation system used: GC - GC Gold Label Luting & Lining); RL - RelyX Luting 2; MC - MaxCem Elite; RU - RelyX U200 and the aging periods (24 hours, 6 months, and 12 months). Slices from the cervical, middle, and apical thirds were obtained and analyzed by push-out bond strength test and confocal laser scanning microscopy. One-way ANOVA and Tukey's post-hoc test was used at a significance level of 5%. For the push-out bond strength test, no differences among GC, RU, and MC in the cervical and middle thirds were observed, regardless of the period of storage (P > 0.05). In the apical third, GC and RU showed similar bond strength but higher than other groups (P > 0.05). After 12 months, GC showed the highest bond strength (P < 0.05). Bond strength to post-space dentin decreased over time, regardless of the cementation system used. Cohesive failure was the most frequent, regardless of the period of storage, cementation system, and post-space third. Tag formation was similar among all groups. After 12 months, GC showed the highest bond strength values.
  • ItemArtigo
    Antimicrobial photodynamic therapy mediated by methylene blue-loaded polymeric micelles against Streptococcus mutans and Candida albicans biofilms
    (2023-03-01) Soares, Jonas Corsino Maduro ; Luiz, Marcela Tavares ; Oshiro Junior, João Augusto ; Besegato, João Felipe ; de Melo, Priscila Borges Gobbo ; Rastelli, Alessandra Nara de Souza ; Chorilli, Marlus ; Universidade Estadual Paulista (UNESP)
    Background: Streptococcus mutans and Candida albicans can colonize the teeth, the oral cavity as biofilm and can cause oral infections. Thus, strategies to prevent and control oral biofilms are requested. The present study aims the development and characterization of methylene blue (MB)-loaded polymeric micelles for antimicrobial photodynamic therapy (aPDT) against Streptococcus mutans and Candida albicans biofilms Methods: MB-loaded polymeric micelles were produced and characterized by particle size, polydispersity index, morphology, zeta potential, stability, MB release profile, and antimicrobial effect against S. mutans and C. albicans biofilms. Results: MB-loaded polymeric micelles showed a reduced particle size, moderate polydisperse profile, spherical and neutral shape, which demonstrated to be promising features to allow micelles penetration into biofilms. Antimicrobial effect against bacterial and yeast biofilms was demonstrated once MB was irradiated by light under 660 nm (aPDT). Furthermore, MB-loaded polymeric micelles showed significant inhibition of S. mutans and C. albicans biofilms. Furthermore, the treatment with MB-micelles incubated with high pre-incubation times (15 and 30 min) were more effective than 5 min. It can be explained by the time required for this nanosystem to penetrate the innermost layer of biofilms and release MB for aPDT. Conclusion: MB-loaded polymeric micelles can effectively decrease the bacteria and yeast viability and it may cause positive impacts in the clinical practice. Thus, the developed formulation showed potential in the treatment to remove oral biofilms, but clinical studies are needed to confirm its potential
  • ItemArtigo
    Cleaning and microstructural effects of amyl acetate on pulp chamber dentin impregnated with epoxy resin-based endodontic sealer
    (2022-12-01) Zaniboni, Joissi Ferrari ; de Souza, Vitor ; Escalante-Otárola, Wilfredo Gustavo ; Leandrin, Thaís Piragine ; Fernández Godoy, Eduardo ; Besegato, João Felipe ; Kuga, Milton Carlos ; Universidade Estadual Paulista (UNESP) ; Universidad Catolica de Santa Maria—UCSM ; Universidad de Chile ; Universidad Autonoma de Chile ; Universidade Paranaense—UNIPAR
    Objective: To evaluate the cleaning potential of 95% ethanol, acetone, and amyl acetate solutions used solely or in association, to remove epoxy resin-based sealer residues from pulp chamber dentin and their microstructural effects. Materials and Methods: One hundred and eighty bovine incisor specimens were divided into nine groups according to the cleaning protocol: ET (ethanol); AC (acetone); AA (amyl acetate); E1: AA+AC; E2: AA+ET; E3: AC+ET; E4: AA+AC+ET; PC (positive control), and NC (negative control). All groups were impregnated with epoxy resin-sealer, except NC. Ninety specimens were divided into groups (n = 10) for evaluation of persistence of residues and amount of open dentinal tubules by SEM analysis and evaluation of chemical compounds on the dentin surface after cleaning with electron dispersive spectroscopy. The others 90 specimens were submitted to Knoop microhardness evaluation. Persistence of residues data were submitted to the Kruskal Wallis and Dunn tests (α = 0.05). Open dentinal tubules and microhardness data were submitted to one-way ANOVA and Mann Whitney tests (α = 0.05). Results: AA and E4 protocols showed the lowest persistence of residues. E4 group had the highest incidence of open dentinal tubules. E3 and E4 groups showed no changes in the atomic ratio Ca/P, which was similar to NC group. E4 group did not present W, an element presents in all the other groups. ET and E4 protocols showed the smallest reduction in dentin microhardness. Conclusions: The combination of amyl acetate, acetone and ethanol is the most effective and safe protocol to remove epoxy sealer residues on pulp chamber dentin. Moreover, it has the lowest microhardness reduction. Clinical Significance: The combined use of amyl acetate, acetone, and ethanol enhanced the cleaning of pulp chamber dentin with minimal microstructural damage.
  • ItemArtigo
    Effect of Different Irrigants on the Adhesive Interface and Influence on the Push Out Strength of Fiber Posts
    (2022-09-01) Belizario, L. G. ; Piragine, T. L. ; Girotto, A. C. ; Gelio, M. B. ; Pereira, J. R. ; Fernandez, E. ; Kuga, M. C. ; Universidade Estadual Paulista (UNESP) ; School of Dentistry Chile ; Universidad Autónoma de Chile
    PURPOSE: The aim of this study was to evaluate the effects of a new irrigant solution on the post space cleaning and the adhesive resistance of fiber posts. METHODS AND MATERIALS: Eighty roots of bovine teeth were randomly allocated into eight groups (n=10 for each group). Evaluations were performed in two different time points for each irrigant. The irrigants included a control group with distilled water (DW), 2.5% NaOCl and 17% EDTA (SH), 1% peracetic acid (PA), and 5% boric acid and 1% citric acid (EX). The time points were 24 hours (I-immediate) and 6 months (D-delayed). The push-out test was performed using a universal testing machine with a 5 kN load cell operating at a crosshead speed of 0.5 mm/minute. The dentinal cementation system was analyzed using a laser confocal microscope (LSM5, Zeiss, Jena, Germany), and incidence of residue on radicular dentin was evaluated by scanning electron microscopy (SEM). The incidence of residue was evaluated by the Kruskal-Wallis test and push-out bond strength and dentin penetrability were evaluated via a one-way analysis of variance (ANOVA) and Tukey tests (α=0.05). RESULTS: The EX irrigation protocol demonstrated the lowest incidence of residue on the dentin surface (p<0.05), independently of the evaluated third. In the cervical and the middle post thirds, EXI, EXD, SHI, and SHD groups exhibited similar push-out bond strength values (p>0.05). EXI, EXD, PAI, and PAD exhibited the greatest dentinal penetrability of the cementation system in all the post thirds (p<0.05). CONCLUSIONS: A solution containing 5% boric acid and 1% citric acid can be a promising irrigant for radicular post space cleaning. It has adequate potential for cleaning the dentin surface without interfering with the adhesive interface between the dentin and the cementation system.
  • ItemArtigo
    Microstructural effect of different concentrations of hydrogen peroxide photoactivated with LED/laser
    (2023-06-01) Manzoli, Tatiane Miranda ; Costa, Joatan Lucas de Sousa Gomes ; Besegato, João Felipe ; Zaniboni, Joissi Ferrari ; Galvani, Lucas David ; Dantas, Andréa Abi Rached ; Kuga, Milton Carlos ; Universidade Estadual Paulista (UNESP) ; Universidade Federal de Minas Gerais (UFMG) ; Universidade Federal de Mato Grosso do Sul (UFMS)
    Background: The use of different concentrations of hydrogen peroxide (HP) photoactivated with LED/laser sources is common however, their influence on tooth structure is not yet fully elucidated. This study aimed to evaluate the pH, microhardness and surface roughness of different bleaching protocols photoactivated with LED/laser. Methods: Forty bovine incisors were sectioned (7 × 7 × 2 mm) and randomized into four groups for analysis of pH (n = 5), microhardness and roughness (n = 10): HP35, HP6_L, HP15_L, HP35_L. The pH analysis was performed in the initial and final minute of the bleaching protocol. Microhardness and roughness were evaluated before and 7 days after the last bleaching session. Results were obtained from two-way ANOVA for repeated measures and Bonferroni post-test at a significance level of 5%. Results: HP6_L showed higher pH and greater stability between the initial and final evaluations, while the other groups showed similar pH with reduced values in the intragroup evaluation. No differences between groups in microhardness and roughness evaluations were observed. Conclusions: Although HP6_L showed higher alkalinity and pH stability, none of the protocols reduced the microhardness and surface roughness of bovine enamel.
  • ItemEditorial
    Editorial: Photonics-based diagnosis and treatment of infectious and inflammatory diseases
    (2023-01-01) Leanse, Leon G. ; Hong, Weili ; de Souza Rastelli, Alessandra Nara ; University of Gibraltar ; Massachusetts General Hospital ; Beihang University ; Universidade Estadual Paulista (UNESP)
  • ItemArtigo
    Effect of calcium hypochlorite as an irrigant alternative in the removal of methylene blue after photodynamic therapy under the post-space adhesive interface
    (2023-05-01) Besegato, João Felipe ; De Sousa Gomes Costa, Joatan Lucas ; Zaniboni, Joissi Ferrari ; Bravo, Giovanna Righetti ; Morais, Jéssika Mayhara Pereira ; Escalante-Otárola, Wilfredo Gustavo ; Kuga, Milton Carlos ; Universidade Federal de Mato Grosso do Sul (UFMS) ; Universidade Estadual Paulista (UNESP) ; Universidad Catolica de Santa Maria - UCSM
    We evaluated the effect of irrigation protocols in removing methylene blue after photodynamic therapy (PDT) in post-space dentin. Bond strength to dentin and failure mode after fiber post cementation was also evaluated. Ninety bovine teeth were endodontically treated. Post-space preparation and PDT mediated by 0.01% methylene blue and diode laser (660 nm) were performed. Three irrigations protocols were tested (n = 10): distilled water (DW), 2.5% sodium hypochlorite (SH), and 6% calcium hypochlorite (CH). Other specimens were used to investigate the bond strength to post-space dentin according to the irrigation protocol and the post cementation system used (RelyX U200 cement (RU) and GC Gold Label 1 glass ionomer cement (GC) in six groups (n = 10): DW-RU, SH-RU, CH-RU, DW-GC, SH-GC e CH-GC. Regardless of the post-space third, DW showed a lower incidence of residues than SH (p < 0.05), but similar to CH. SH showed similar results to CH (p > 0.05). Regarding the bond strength, CH-RU and CH-GC showed higher values compared to the other groups (p< 0.05), but similar to each other. No differences were observed between the other groups (p > 0.05). Cohesive was the most predominant failure. 6% CH to remove methylene blue after PDT favors the bond strength of RelyX U200 and GC Gold Label.
  • ItemArtigo
    Effect of foraminal enlargement on microcrack formation and apical transportation: a nano-CT assessment
    (2023-12-01) Pinto, Jader Camilo ; de Faria-Vasconcelos, Karla ; Leite, André Ferreira ; Pedano, Mariano Simón ; Guerreiro-Tanomaru, Juliane ; Jacobs, Reinhilde ; Tanomaru-Filho, Mario ; Universidade Estadual Paulista (UNESP) ; University Hospitals Leuven ; University of Brasília ; Dentistry ; Karolinska Institutet
    The aim of this study was to evaluate the foraminal enlargement and its influence on microcrack formation and apical transportation in root canals with apical curvature. Eighteen maxillary lateral incisors with apical curvature were selected by using micro-CT images. Root canals were randomly divided in two groups (n = 9) according to root canal preparation using two working lengths: 1 mm short of the apical foramen (control group) and 1 mm beyond the apical foramen (foraminal enlargement). For both groups Reciproc Blue R40 was used for root canal instrumentation. Specimens were scanned by nano-CT (UniTOM HR) before and after root canal preparation. Percentage, length, and width of microcracks, and apical transportation were assessed. Kappa, chi‐square and McNemar tests were used for qualitative analyses while paired and unpaired t-test were used for quantitative analyses (α = 0.05). For both groups, rather similar and low percentages of microcracks were observed before root canal preparation (P > 0.05). The foraminal enlargement promoted new microcracks, not observed in the control group. An increase in microcrack length was observed when the foraminal enlargement was performed (P < 0.05). Higher apical transportation was observed when foraminal enlargement was performed (P < 0.05). Foraminal enlargement using a heat-treated reciprocating file size 40 promoted microcracks and higher apical transportation than root canal preparation up to 1 mm short of apical foramen.
  • ItemArtigo
    Physicochemical properties, cytotoxicity and bioactivity of a ready-to-use bioceramic repair material
    (2023-01-01) Campi, Lívia Bueno ; Rodrigues, Elisandra Márcia ; Torres, Fernanda Ferrari Esteves ; Reis, José Maurício Dos Santos Nunes ; Guerreiro-Tanomaru, Juliane Maria ; Tanomaru-Filho, Mário ; Universidade Estadual Paulista (UNESP)
    The aim of this study was to evaluate the physicochemical properties, cytotoxicity and bioactivity of a ready-to-use bioceramic material, Bio-C Repair (Angelus), in comparison with White MTA (Angelus) and Biodentine (Septodont). The physicochemical properties of setting time, radiopacity, pH, solubility, dimensional and volumetric changes were evaluated. Biocompatibility and bioactivity were assessed in Saos-2 osteoblast cell cultures by the MTT assay 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), Neutral Red (NR), Alizarin Red (ARS), and cell migration tests. Statistical analysis was performed by ANOVA, Tukey or Bonferroni tests (α = 0.05). Bio-C Repair had the longest setting time (p < 0.05), but radiopacity and solubility were accordance with the ISO 6876/2012 standards, besides linear expansion. Bio-C Repair and MTA had similar volumetric change (p > 0.05); lower than Biodentine (p < 0.05). All the materials evaluated had an alkaline pH. Bio-C Repair was cytocompatible and promoted mineralized nodule deposition in 21 days and cell migration in 3 days. In conclusion, Bio-C Repair had adequate radiopacity above 3mm Al, solubility less than 3%, dimensional expansion, and low volumetric change. In addition, Bio-C Repair promoted an alkaline pH and presented bioactivity and biocompatibility similar to MTA and Biodentine, showing potential for use as a repair material.
  • ItemArtigo
    Effectiveness and color stability of non-vital dental bleaching photoactivated by violet LED on blood-stained teeth
    (2023-06-01) de Almeida, Eran Nair Mesquita ; Silva, Aryvelto Miranda ; Besegato, João Felipe ; de Sousa Gomes Costa, Joatan Lucas ; Manzoli, Tatiane Miranda ; de Andrade, Marcelo Ferrarezi ; Kuga, Milton Carlos ; Universidade Estadual Paulista (UNESP) ; Federal University of Juiz de Fora (UFJF) ; Universidade Federal de Mato Grosso do Sul (UFMS)
    Background: Few studies have investigated the effect of violet LED irradiation associated or not with bleaching agents on blood-stained teeth. This in vitro study aimed to evaluate the whitening efficacy and color stability of non-vital dental bleaching using 35% hydrogen peroxide (HP) photoactivated with violet LED (VL) compared to 35% HP alone and 35% HP photoactivated with blue LED (BL). Methods: Fifty bovine dental crowns were used to obtain specimens of 5 × 5 × 2 mm. After selection based on a previous colorimetric analysis, the specimens were blood-stained and randomly assigned into five groups (n = 10): control (no treatment); 35% HP, 35% HP/BL; 35% HP/VL; and VL. Three bleaching sessions were performed and the colorimetric analysis (∆Eab, ∆L, and ∆WID) was recorded after 7 days, 30 days, and 9 months of the last bleaching session. Two-way repeated measures ANOVA followed by Bonferroni post-hoc test was used at a significance level of 5%. Results: 35% HP, 35% HP/BL, and 35% HP/VL showed higher values of ∆Eab, ∆L, e ∆WID (P < 0.05), without intra- and intergroup differences (P > 0.05). C and VL were similar in all the evaluation times (P > 0.05), showing lower values of ∆Eab, ∆L, and ∆WID (P < 0.05). Conclusions: 35% HP/VL can be a viable alternative for dental bleaching in endodontically-treated teeth, showing bleaching efficacy similar to 35% HP solely used, even after a 9-month follow-up. VL used alone was not effective to bleach blood-stained teeth.
  • ItemArtigo
    The Influence on Fracture Resistance of Different Composite Resins and Prefabricated Posts to Restore Endodontically Treated Teeth
    (2023-01-01) Pamato, Saulo ; Ricci, Weber Adad ; Kuga, Milton Carlos ; de Oliveira, Eliane Cristina Gulin ; Moraes, João Carlos Silos ; Só, Marcus Vinicius Reis ; Trevisan, Tamara Carolina ; Júnior, Newton Fahl ; Pereira, Jefferson Ricardo ; University of Southern Santa Catarina (UNISUL) ; Universidade Estadual Paulista (UNESP) ; University Federal of Rio Grande do Sul (UFRGS) ; Private Practice
    Recent formulations of resin-based composites have incorporated different combinations of materials. However, the mechanical and bonding behavior of these materials with intraradicular posts are unclear. This study aimed to evaluate the effect of light-cure and dual-cure resin composite posts on the fracture resistance of endodontically-treated teeth. Materials and Methods: Ninety extracted human upper canines were selected and randomly divided into nine groups (n=10): (G1) endodontically treated teeth without endodontic posts; (G2) glass-fiber post cemented with glass-ionomer cement; (G3) endodontic post by dual-cure composite resin (Rebilda DC); (G4) endodontic post by dual-cure composite resin (Cosmecore); (G5) endodontic post by dual-cure composite resin (Bis-Core); (G6) endodontic post by light-cure composite resin; (G7) glass-fiber post customized with flowable composite resin; (G8) glass-fiber post cemented with light-cure composite resin; (G9) glass-fiber post cemented with self-adhesive resin cement. After the post insertion, all specimens were subjected to mechanical (250,000 cycles) and thermocycling (6000 cycles, 5 °C/55 °C) and immediate loading at 45 degrees in a universal testing machine until fracture. The data were analyzed by one-way ANOVA and multiple comparisons using the Fisher LSD Method (p < 0 05). Results: The mean failure loads (±SD) for the groups ranged from 100.7 ± 22.6 N to 221.9 ± 48.9 N. The G1 group (without endodontic posts) had a higher fracture strength than all experimental groups (p < 0.001). Conclusions: Within the limitations, the light- and dual-cure post technique did not present lower fracture resistance values as compared to the conventional glass-fiber post.
  • ItemArtigo
    Strontium Carbonate and Strontium-Substituted Calcium Carbonate Nanoparticles Form Protective Deposits on Dentin Surface and Enhance Human Dental Pulp Stem Cells Mineralization
    (2022-12-01) Dotta, Tatiane Cristina ; Hayann, Larwsk ; de Padua Andrade Almeida, Leonardo ; Nogueira, Lucas Fabrício B. ; Arnez, Mayara M. ; Castelo, Raisa ; Cassiano, Ana Flávia B. ; Faria, Gisele ; Martelli-Tosi, Milena ; Bottini, Massimo ; Ciancaglini, Pietro ; Catirse, Alma B. C. E. B. ; Ramos, Ana Paula ; Universidade de São Paulo (USP) ; Universidade Estadual Paulista (UNESP) ; University of Rome Tor Vergata
    Strontium acetate is applied for dental hypersensitivity treatment; however, the use of strontium carbonates for this purpose has not been described. The use of Sr-carbonate nanoparticles takes advantage of both the benefits of strontium on dentin mineralization and the abrasive properties of carbonates. Here in, we aimed to synthesize strontium carbonate and strontium-substituted calcium carbonate nanoparticles and test them as potential compounds in active dentifrices for treating dental hypersensitivity. For this, SrCO3, Sr0.5Ca0.5CO3, and CaCO3 nanoparticles were precipitated using Na2CO3, SrCl2, and/or CaCl2 as precursors. Their morphology and crystallinity were evaluated by electron microscopy (SEM) and X-ray diffraction, respectively. The nanoparticles were added to a poly (vinyl alcohol) gel and used to brush dentin surfaces isolated from human third molars. Dentin chemical composition before and after brushing was investigated by infrared spectroscopy (FTIR) and X-ray dispersive energy spectroscopy. Dentin tubule morphology, obliteration, and resistance of the coatings to acid attack were investigated by SEM and EDS. The cytotoxicity and ability of the particles to trigger the mineralization of hDPSCs in vitro were studied. Dentin brushed with the nanoparticles was coated by a mineral layer that was also able to penetrate the tubules, while CaCO3 remained as individual particles on the surface. FTIR bands related to carbonate groups were intensified after brushing with either SrCO3 or Sr0.5Ca0.5CO3. The shift of the phosphate-related FTIR band to a lower wavenumber indicated that strontium replaced calcium on the dentin structure after treatment. The coating promoted by SrCO3 or Sr0.5Ca0.5CO3 resisted the acid attack, while calcium and phosphorus were removed from the top of the dentin surface. The nanoparticles were not toxic to hDPSCs and elicited mineralization of the cells, as revealed by increased mineral nodule formation and enhanced expression of COL1, ALP, and RUNX2. Adding Sr0.5Ca0.5CO3 as an active ingredient in dentifrices formulations may be commercially advantageous since this compound combines the well-known abrasive properties of calcium carbonate with the mineralization ability of strontium, while the final cost remains between the cost of CaCO3 and SrCO3. The novel Sr0.5Ca0.5CO3 nanoparticles might emerge as an alternative for the treatment of dental hypersensitivity.
  • ItemArtigo
    Cloner 3D photogrammetric facial scanner: Assessment of accuracy in a controlled clinical study
    (2023-04-01) Nogueira, Básia R. ; Oliveira Junior, Osmir B. ; de Sousa Gomes Costa, Joatan L. ; Zanetti, Thomaz Faraco ; Pretel, Hermes ; Universidade Estadual Paulista (UNESP) ; Universidade de São Paulo (USP) ; Engineering Director of DONE 3D
    Objective: To evaluate the accuracy of facial measurements on three-dimensional images obtained using a new photogrammetric scanner. Material and Methods: A total of 11 participants were included in the study. Nine customized adhesive labels were used to identify the facial landmarks: Trichion (Tri), Glabella (G), Right (Exr) and Left (Exl), Pronasal (Pn), Subnasal (Sn), Chelion right (Chr) and left (Chl) and Mentonian (Me). Two trained and calibrated examiners were responsible for performing seven linear measurements for each participant (Tri-G, Sn-Me, Exr-Exl, Chr-Chl, Exr-Chr, Exl-Chl, Pn-Sn) first with a digital caliper and later with a three-dimensional model obtained after digitalization with photogrammetric technology. The intraclass correlation coefficient (ICC), mean difference, SD, and Bland–Altman correlation were used to compare the measurements performed. Results: Intra and inter-examiner reliability were excellent (ICC >0.9). In general, the measurements presented a variation of a minor 2.0 mm. However, only three measures (Sn-Me, Exr-Exl, and Exr-Chr) were outside the clinical acceptability range. Conclusions: The 3D Cloner scanner showed clinically acceptable accuracy comparable to the digital caliper with a variation of −0.8 ± 1.2 mm. Inter- and intra-examiner agreement on digital measurements was also observed. Clinical Significance: Scanners with accurate 3D model reproductions associated with reliable digital measurements provide a more precise diagnosis and better planning in orofacial treatment.
  • ItemArtigo
    The Effect of Octenidine on Proliferation, Migration, and Osteogenic Differentiation of Human Dental Pulp and Apical Papilla Stem Cells
    (2022-12-01) Cassiano, Ana Flávia Balestrero ; Coaguila-Llerena, Hernán ; Santos, Cíntia Silva ; da Silva, Luana Raphael ; Nogueira, Lucas Fabrício Bahia ; Ciancaglini, Pietro ; Faria, Gisele ; Universidade Estadual Paulista (UNESP) ; School of Dentistry ; Universidade de São Paulo (USP)
    Introduction: The research for alternative irrigating solutions is ongoing, since no “ideal” solution has yet been found. Octenidine dihydrochloride (OCT) has been indicated as an endodontic irrigant because it has adequate antimicrobial and biological properties. The present study aimed to assess the effects of OCT on proliferation, migration, and induction of the osteogenic phenotype of stem cells from human dental pulp and apical papilla. Methods: Cells were collected from human third molars and exposed to different doses of OCT, chlorhexidine (CHX), sodium hypochlorite (NaOCl), and ethylenediaminetetraacetic acid (EDTA) to determine cell viability by alamarBlue assay; proliferation by bromodeoxyuridine incorporation; migration by the Transwell assay; alkaline phosphatase activity by thymolphthalein release; and production of mineralized nodules by alizarin red staining. The results were analyzed by 1- or 2-way analysis of variance and Tukey (α =.05). Results: CHX promoted lower cell viability, followed by OCT, NaOCl, and EDTA, especially at intermediate doses (P <.05). Cells exposed to CHX had less proliferation than the other groups (P <.05). The Transwell assay revealed no differences among OCT, EDTA, and culture medium (control group) (P >.05). OCT and EDTA induced greater migration than CHX and NaOCl (P <.05). OCT and EDTA induced higher alkaline phosphatase activity than NaOCl and CHX (P <.05). No difference was detected among the groups using alizarin red staining (P >.05). Conclusions: OCT induced high migration, proliferation, and alkaline phosphatase activity of stem cells from human dental pulp and apical papilla, which could be advantageous for regenerative endodontic procedures.
  • ItemArtigo
    Evaluation of in vitro experimental model for analysis of bioceramic sealers
    (2022-01-01) Silva, Ericke Mucke ; Alcalde, Murilo Priori ; Vivan, Rodrigo Ricci ; Pomini, Marcelo ; Tanomaru-Filho, Mario ; Duarte, Marco Antonio Hungaro ; Universidade de São Paulo (USP) ; Bauru Dental School ; Universidade Estadual Paulista (UNESP)
    This study aimed to evaluate physicochemical properties of three ready-to-use calcium silicate-based endodontic sealers: Endosequence BC Sealer, Bio C Sealer, and Sealer Plus BC. Radiopacity was evaluated using specimens of 10 mm in diameter and 1 mm in height, along with an aluminum step wedge. For the flow test, 0.5 mL of each sealer was deposited between glass plates and the mean of the measurement of two diameters was considered the cement flow value. For pH and calcium release, root canals of 30 prototypes of upper incisor acrylic resin teeth were filled with sealer and gutta percha point and then immersed in containers with 13 mL of ultrapure water. Both pH and release of calcium ions (atomic absorption spectrophotometer) were measured at 3, 24, 72, and 168 h. Acrylic resin teeth were scanned by Micro-CT 1174 at the time of pH and calcium ion readings for volumetric change analysis. The data were analyzed by ANOVA, Tukey’s, Kruskal-Wallis, and Dunn’s tests. Endosequence BC Sealer presented the lowest, and Bio C Sealer the highest volumetric change after 72 h and 168 h (p < 0.05). Endosequence BC Sealer presented higher radiopacity (p < 0.05). All materials showed alkalinization capacity. All of them presented calcium ion release, with a higher value for Sealer Plus BC. All materials presented alkalinization, calcium release capacity, radiopacity, and flow above the minimum values required by the ISO standard. The highest volumetric loss was experienced by Bio C Sealer and the lowest one by Endosequence BC Sealer.
  • ItemArtigo
    Effect of Curing Modes on the Mechanical Properties of Commercial Dental Resin-Based Composites: Comparison between Different LEDs and Microwave Units
    (2022-10-01) Vermudt, Alef ; Kuga, Milton Carlos ; Besegato, João Felipe ; Oliveira, Eliane Cristina Gulin de ; Leandrin, Thaís Piragine ; Só, Marcus Vinicius Reis ; Moraes, João Carlos Silos ; Pereira, Jefferson Ricardo ; University of Southern Santa Catarina–Unisul ; Universidade Estadual Paulista (UNESP) ; University Federal of Rio Grande do Sul (UFRGS)
    Resin-based composites (RBCs) have transformed restorative dentistry and its procedures. However, the characteristics of RBCs have been modified over the years to enhance the physical and chemical properties of the materials. This context raises the need for studies that evaluate whether the properties of the RBCs that are commercially available are clinically adequate with different curing modes. This study aimed to evaluate the mechanical behavior of commercial RBCs after undergoing different curing modes. Twenty-three RBCs of different classes were evaluated. For curing the specimens, a microwave (BMS45, Brastemp) (for 3 min at 450 W) and three LED units were used: an Emitter A Fit (Schuster (second generation)) (light-curing for 15 s with an irradiance of 1250 mW/cm2), VALO (Ultradent (third generation)) (light-curing for 15 s with an irradiance of 1100 mW/cm2), and Emitter Now Duo (Schuster (second generation)) (light-curing for 15 s with an irradiance of 1100 mW/cm2). A total of 670 RBC specimens of 8 mm in diameter and 1 mm in depth were obtained. Afterward, a biaxial flexure strength test was performed until the failure of the specimens, using a universal testing machine set at a speed of 0.5 mm/min. The same specimens were subjected to infrared spectroscopy for evaluating the degree of conversion. Tukey’s test was used for multiple comparisons at a significance level of 5%. The light-curing mode did not affect the flexure strength of the RBCs (p > 0.05), but the type and shade of RBCs did so (p < 0.05). In conclusion, the type of RBC directly interferes with the mechanical behavior of the material. However, the curing modes within the same RBC did not change the mechanical properties.
  • ItemArtigo
    Violet LED associated with high concentration hydrogen peroxide: Effects on bleaching efficacy, pH, and temperature
    (2022-12-01) Manzoli, Tatiane Miranda ; Costa, Joatan Lucas de Sousa Gomes ; Besegato, João Felipe ; Gelio, Mariana Bena ; Galvani, Lucas David ; Bordini, Ester Alves Ferreira ; Kuga, Milton Carlos ; Dantas, Andréa Abi Rached ; Universidade Estadual Paulista (UNESP) ; Universidade de São Paulo (USP)
    Background: This study aimed to evaluate the bleaching efficacy, pH, and temperature of 35% hydrogen peroxide (HP) gel used alone or associated with violet LED. Methods: Sixty bovine crowns were sectioned (5 × 5 × 2mm). After staining with black tea, the specimens were randomized into four groups (n = 10) according to the bleaching protocol: HP35R: 3 × 15 min 35% HP; HP35: 1 × 45 min 35% HP; HP35VR: 3 × 8min 35% HP + Violet LED; HP35V: 1 × 24 min + Violet LED. Two bleaching sessions were performed for all the groups. Color change was evaluated before, 24h after each session, 7 days and 15 days after the last session. The variables ∆E00 [CIEDE2000] and WID were used for color analysis. The pH variation (initial and final) and the temperature of the gel were recorded (n = 5). ANOVA two-way for repeated measures and Bonferroni post-test was used at a significance level of 5%. Results: HP35VR and HP35V the most noticeable color change(p < 0.05). The final values of pH were lower than the initial ones, but with no difference between the groups (p > 0.05). Groups HP35VR and HP35V showed an increase in temperature in relation to HP35R (p < 0.05). Conclusions: Violet LED improved the bleaching efficacy of 35% HP in a time-saving manner without negatively affecting the pH and temperature of 35% HP. The renewal of HP did not influence the outcomes.
  • ItemArtigo
    Effect of non-vital tooth bleaching photoactivated with blue or violet LED on color and microhardness
    (Elsevier B.V., 2023-04-21) Almeida, Eran Nair Mesquita de ; Costa, Joatan Lucas de Sousa Gomes ; Besegato, Joao Felipe ; Silva, Aryvelto Miranda ; Manzoli, Tatiane Miranda ; Vitoria, Matheus Sousa ; Andrade, Marcelo Ferrarezi de ; Kuga, Milton Carlos ; Universidade Estadual Paulista (UNESP) ; Universidade Federal de Mato Grosso do Sul (UFMS) ; Univ Fed Juiz de Fora
    Background: To evaluate the efficacy of dental bleaching protocols using 35% hydrogen peroxide photoactivated with violet LED on color and microhardness of endodontically treated teeth. Methods: Forty specimens were selected and randomized into 4 groups (n = 10): C -Control, HP -35% hydrogen peroxide, HP + BL -35% hydrogen peroxide + blue LED, HP + VL -35% hydrogen peroxide + violet LED. Three bleaching sessions were performed for each group. Color analysis was performed 7 days after each bleaching session. Two-way repeated measure ANOVA and Bonferroni test were used to evaluate the effect of different bleaching protocols and evaluation times on the dependent variables (Delta E and Delta L). Dentin microhardness was measured 24 h after the third bleaching session. Data were evaluated by ANOVA and Tukey's test at a signifi-cance level of 5%. Results: Differences on Delta E and Delta L were verified after the first and second bleaching sessions (p < 0.05) and showed stability after the third one, for all the groups. No differences were observed among HP, HP + BL, and HP + VL groups, regardless of the evaluation time (p > 0.05). HP and C showed the greatest and smallest reduction in dentin microhardness (p < 0.05), respectively. No difference between HP + BL and HP + VL pro-tocols (P > 0.05) was observed. Conclusions: High concentration hydrogen peroxide (35%) photoactivated with violet LED bleached endodonti-cally treated teeth effectively. However, the same protocol negatively affected the dentin microhardness, but not in the same level of 35% HP solely used.
  • ItemArtigo
    Effect of irrigation protocols on chemical smear layer formation over the post-space dentin
    (2022-08-01) Gelio, Mariana Bena ; Ramos, Anna Thereza Peroba Rezende ; Zaniboni, Joissi Ferrari ; Escalante-Otárola, Wilfredo Gustavo ; Besegato, João Felipe ; Kuga, Milton Carlos ; Universidade Estadual Paulista (UNESP) ; Universidad Catolica de Santa Maria – UCSM
    This study evaluated the effect of irrigation protocols using 95% ethanol (ET) or 1% peracetic acid (PA) prior the use of 2% chlorhexidine (CHX) compared to distilled water (DW) on the chemical smear layer (CSL) formation and incidence of open dentin tubules at the apical, medium, and cervical third of the post-space dentin. Scanning electron microscopy (SEM) and electron dispersive spectroscopy (EDS) images were used. Forty bovine roots were endodontically treated. After, post-space preparation was performed and the roots were randomized in four groups (n = 10) according to the irrigation protocol: DW, CHX, CHX-ET and CHX-PA. The chemical composition of CSL and the incidence of open dentin tubules at the post-space thirds were evaluated by EDS (500× magnification) and SEM (2000× magnification) images, respectively. Data from chemical composition of CSL were descriptively analyzed, while the incidence of open dentin tubules was evaluated by scores and submitted to Kruskal-Wallis and Dunn test (p =.05). Cl, Bi, and Si were the chemical elements most found over the dentin after the irrigation with CHX and CHX-ET. Moreover, CHX and CHX-ET showed the highest incidence of CSL (p <.05), but without difference between them (p >.05), regardless of the post-space third. DW and CHX-PA showed similar incidence of CSL (p >.05). No difference on the incidence of open dentin tubules was found for any irrigation protocol and post-space third (p >.05). The use of 1% PA prior the post-space irrigation with CHX decrease the incidence of CSL. Research Highlights: The post-space irrigation with chlorhexidine results in the formation of chemical smear layer. Ethanol is not capable to remove the chemical smear layer. Peracetic acid is more effective to remove the chemical smear layer.