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Melatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cells

dc.contributor.authorLopes, Juliana [UNESP]
dc.contributor.authorArnosti, David
dc.contributor.authorTrosko, James E.
dc.contributor.authorTai, Mei-Hui
dc.contributor.authorZuccari, Debora [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionMichigan State University
dc.contributor.institutionFaculdade de Medicina de São José do Rio Preto
dc.date.accessioned2022-04-29T08:45:03Z
dc.date.available2022-04-29T08:45:03Z
dc.date.issued2016-05-01
dc.description.abstractCancer stem cells (CSCs) pose a challenge in cancer treatment, as these cells can drive tumor growth and are resistant to chemotherapy. Melatonin exerts its oncostatic effects through the estrogen receptor (ER) pathway in cancer cells, however its action in CSCs is unclear. Here, we evaluated the effect of melatonin on the regulation of the transcription factor OCT4 (Octamer Binding 4) by estrogen receptor alpha (ERα) in breast cancer stem cells (BCSCs). The cells were grown as a cell suspension or as anchorage independent growth, for the mammospheres growth, representing the CSCs population and treated with 10 nM estrogen (E2) or 10 µM of the environmental estrogen Bisphenol A (BPA) and 1 mM of melatonin. At the end, the cell growth as well as OCT4 and ERα expression and the binding activity of ERα to the OCT4 was assessed. The increase in number and size of mammospheres induced by E2 or BPA was reduced by melatonin treatment. Furthermore, binding of the ERα to OCT4 was reduced, accompanied by a reduction of OCT4 and ERα expression. Thus, melatonin treatment is effective against proliferation of BCSCs in vitro and impacts the ER pathway, demonstrating its potential therapeutic use in breast cancer.en
dc.description.affiliationDepartment of Biology Universidade Estadual Paulista “Júlio de Mesquita Filho”
dc.description.affiliationDepartment of Biochemistry and Molecular Biology Michigan State University
dc.description.affiliationDepartment of Pediatrics and Human Development Michigan State University
dc.description.affiliationDepartment of Molecular Biology Faculdade de Medicina de São José do Rio Preto
dc.description.affiliationUnespDepartment of Biology Universidade Estadual Paulista “Júlio de Mesquita Filho”
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent209-217
dc.identifierhttp://dx.doi.org/10.18632/genesandcancer.107
dc.identifier.citationGenes and Cancer, v. 7, n. 5-6, p. 209-217, 2016.
dc.identifier.doi10.18632/genesandcancer.107
dc.identifier.issn1947-6027
dc.identifier.issn1947-6019
dc.identifier.scopus2-s2.0-85006646858
dc.identifier.urihttp://hdl.handle.net/11449/231390
dc.language.isoeng
dc.relation.ispartofGenes and Cancer
dc.sourceScopus
dc.subjectChromatin immunoprecipitation
dc.subjectEstrogen receptor
dc.subjectMammospheres
dc.subjectMelatonin
dc.subjectThree-dimensional growth
dc.titleMelatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cellsen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBiologia - IBILCEpt

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