Antibiofilm and anti-caries effects of an experimental mouth rinse containing Matricaria chamomilla L. extract under microcosm biofilm on enamel

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dc.contributor.authorBraga, Aline Silva
dc.contributor.authorSimas, Leticia Lobo de Melo
dc.contributor.authorPires, Juliana Gonçalves
dc.contributor.authorSouza, Beatriz Martines
dc.contributor.authorde Melo, Fernanda Pereira de Souza Rosa [UNESP]
dc.contributor.authorSaldanha, Luiz Leonardo [UNESP]
dc.contributor.authorDokkedal, Anne Ligia [UNESP]
dc.contributor.authorMagalhães, Ana Carolina
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-12T02:25:06Z
dc.date.available2020-12-12T02:25:06Z
dc.date.issued2020-08-01
dc.description.abstractObjective: This study evaluated the antibiofilm and anti-caries effects of an experimental mouth rinse containing aqueous extract of Matricaria chamomilla L. Methods: Microcosm biofilm was produced on bovine enamel, from pooled human saliva mixed with McBain saliva, under 0.2 % sucrose exposure, for 5 days. The biofilm was daily treated using (1 mL/1 min): Vochysia tucanorum Mart. (2.5 mg/mL); Myrcia bella Cambess. (1.25 mg/mL); Matricaria chamomilla L. (20 mg/mL); Malva sylvestris (Malvatricin® Plus–Daudt); 0.12 % Chlorhexidine (PerioGard®-Palmolive, Positive control) and PBS (Negative control). The % dead bacteria, biofilm thickness, EPS biovolume, lactic acid concentration, the CFU counting (total microorganisms, Lactobacillus sp., total streptococci and Streptococcus mutans/S. sobrinus) were determined. Enamel demineralization was measured by TMR. Results: All mouth rinses induced bacterial death compared to PBS (p < 0.0001). The biofilm thickness varied from 12 ± 2 μm (chlorhexidine) to 18 ± 2 μm (V. tucanorum) (ANOVA/Tukey, p < 0.0001). The EPS biovolume varied from 7(4)% (chlorhexidine) to 30(20)% (PBS) (Kruskal-Wallis/Dunn, p < 0.0001). The lactic acid production was reduced by M. sylvestris (1.1 ± 0.2 g/L) and chlorhexidine (0.6 ± 0.2 g/L) compared to PBS (2.6 ± 1.3 g/L) (ANOVA, p < 0.0001). Malva sylvestris and chlorhexidine showed significant low CFU for total microorganisms, Lactobacillus sp. and total streptococci. Only chlorhexidine significantly reduced S. mutans/S. sobrinus. CFUs for total streptococci and Lactobacillus sp, were also significantly reduced by M. chamomilla L. Malva sylvestris (63.4 % of mineral loss reduction), chlorhexidine (47.4 %) and M. chamomilla L. (39.4 %) significantly reduced enamel demineralization compared to PBS (ANOVA/Tukey, p < 0.0001). Conclusion: M. chamomilla L. has lower antibiofilm action, but comparable anti-caries effect to those found for chlorhexidine, under this model. Clinical relevance: This study shows that the antibiofilm and anti-caries potential may vary between the commercial and experimental mouth rinses containing natural agents, with promising results for those containing Matricaria chamomilla L. and Malva Sylvestris.en
dc.description.affiliationDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo
dc.description.affiliationDepartment of Biological Sciences School of Science The São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Biological Sciences School of Science The São Paulo State University (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2020.103415
dc.identifier.citationJournal of Dentistry, v. 99.
dc.identifier.doi10.1016/j.jdent.2020.103415
dc.identifier.issn0300-5712
dc.identifier.scopus2-s2.0-85087415932
dc.identifier.urihttp://hdl.handle.net/11449/201141
dc.language.isoeng
dc.relation.ispartofJournal of Dentistry
dc.sourceScopus
dc.subjectDental caries
dc.subjectEnamel
dc.subjectMicrocosm biofilm
dc.subjectMouth wash
dc.subjectNatural agents
dc.titleAntibiofilm and anti-caries effects of an experimental mouth rinse containing Matricaria chamomilla L. extract under microcosm biofilm on enamelen
dc.typeArtigo
unesp.author.orcid0000-0003-2454-067X[6]
unesp.author.orcid0000-0002-6413-5348[8]
unesp.departmentCiências Biológicas - FCpt

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