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Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca

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dc.contributor.authorMorais, Glaucia Almeida de
dc.contributor.authorTakaki, Massanori [UNESP]
dc.contributor.institutionUniversidade Estadual de Mato Grosso do Sul (UEMS)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:07:27Z
dc.date.available2014-05-20T15:07:27Z
dc.date.issued1998-06-01
dc.description.abstractDetermination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.en
dc.description.affiliationUEMS
dc.description.affiliationUNESP Departamento de Botânica
dc.description.affiliationUnespUNESP Departamento de Botânica
dc.format.extent17-25
dc.identifierhttp://dx.doi.org/10.1590/S1516-89131998000100003
dc.identifier.citationBrazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 41, n. 1, p. 17-25, 1998.
dc.identifier.doi10.1590/S1516-89131998000100003
dc.identifier.fileS1516-89131998000100003.pdf
dc.identifier.issn1516-8913
dc.identifier.lattes1474830375607171
dc.identifier.scieloS1516-89131998000100003
dc.identifier.urihttp://hdl.handle.net/11449/26518
dc.language.isoeng
dc.publisherBrazilian Archives of Biology and Technology
dc.relation.ispartofBrazilian Archives of Biology and Technology
dc.relation.ispartofjcr0.676
dc.relation.ispartofsjr0,281
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectdeterminationen
dc.subjectselective inactivationen
dc.subjectPhaseolus vulgarisen
dc.titleDetermination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. cariocaen
dc.typeArtigo
dspace.entity.typePublication
unesp.author.lattes1474830375607171
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt
unesp.departmentBotânica - IBpt

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Rio Claro - IB - Instituto de Biociências