Sources of errors in the quantitative analysis of food carotenoids by HPLC

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dc.contributor.authorKimura, M.
dc.contributor.authorRodriguez-Amaya, D. B.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:01:51Z
dc.date.available2014-05-20T14:01:51Z
dc.date.issued1999-09-01
dc.description.abstractSeveral factors render carotenoid determination inherently difficult. Thus, in spite of advances in analytical instrumentation, discrepancies in quantitative results on carotenoids can be encountered in the international literature. A good part of the errors comes from the pre-chromatographic steps such as sampling scheme that does not yield samples representative of the food lots under investigation; sample preparation which does not maintain representativity and guarantee homogeneity of the analytical sample; incomplete extraction; physical losses of carotenoids during the various steps, especially during partition or washing and by adsorption to glass walls of containers; isomerization and oxidation of carotenoids during analysis. on the otherhand, although currently considered the method of choice for carotenoids, high performance liquid chromatography (HPLC) is subject to various sources of errors, such as: incompatibility of the injection solvent and the mobile phase, resulting in distorted or split peaks; erroneous identification; unavailability, impurity and instability of carotenoid standards; quantification of highly overlapping peaks; low recovery from the HPLC column; errors in the preparation of standard solutions and in the calibration procedure; calculation errors. Illustrations of the possible errors in the quantification of carotenoids by HPLC are presented.en
dc.description.affiliationUniv Estadual Paulista, IBILCE, Dept Engn & Tecnol Alimentos, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, IBILCE, Dept Engn & Tecnol Alimentos, Sao Jose do Rio Preto, SP, Brazil
dc.format.extent58S-66S
dc.identifierhttp://www.scielo.org.ve/scielo.php?script=sci_issues&pid=0004-0622&lng=es&nrm=iso
dc.identifier.citationArchivos Latinoamericanos de Nutricion. Caracas: Archivos Latinoamericanos Nutricion, v. 49, n. 3, p. 58S-66S, 1999.
dc.identifier.issn0004-0622
dc.identifier.lattes2298375076173727
dc.identifier.urihttp://hdl.handle.net/11449/21828
dc.identifier.wosWOS:000088005700009
dc.language.isoeng
dc.publisherArchivos Latinoamericanos Nutricion
dc.relation.ispartofArchivos Latinoamericanos de Nutrición
dc.relation.ispartofjcr0.358
dc.relation.ispartofsjr0,179
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcarotenoidspt
dc.subjectquantitative analysispt
dc.subjectHPLCpt
dc.subjectanalytical errorspt
dc.titleSources of errors in the quantitative analysis of food carotenoids by HPLCen
dc.typeArtigo
dcterms.licensehttp://www.scielo.org.ve/revistas/alan/eaboutj.htm
dcterms.rightsHolderArchivos Latinoamericanos Nutricion
unesp.author.lattes2298375076173727
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Engenharia e Tecnologia de Alimentos - IBILCE