Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus)

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dc.contributor.authorGemeiner, Hendryk [UNESP]
dc.contributor.authorMenegario, Amauri Antonio [UNESP]
dc.contributor.authorEismann, Carlos Eduardo [UNESP]
dc.contributor.authorElias, Lucas Pellegrini [UNESP]
dc.contributor.authorPedrobom, Jorge Henrique [UNESP]
dc.contributor.authorDourado, Thiago de Araujo [UNESP]
dc.contributor.authorChang, Hung Kiang [UNESP]
dc.contributor.authorConceicao, Fabiano Tomazini da [UNESP]
dc.contributor.authorMoruzzi, Rodrigo Braga [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T12:00:11Z
dc.date.available2023-07-29T12:00:11Z
dc.date.issued2023-04-03
dc.description.abstractThis work aims to evaluate the size and lability of Cu and Zn bound to proteins in the cytosol of fish liver of Oreochromis niloticus by employing solid-phase extraction (SPE), diffusive gradients in thin films (DGT), and ultrafiltration (UF). SPE was carried out using Chelex-100. DGT containing Chelex-100 as binding agent was employed. Analyte concentrations were determined by ICP-MS. Total Cu and Zn concentrations in cytosol (1 g of fish liver in 5 ml of Tris-HCl) ranged from 39.6 to 44.3 ng ml(-1) and 1498 to 2106 ng ml(-1), respectively. Data from UF (10-30 kDa) suggested that Cu and Zn in cytosol were associated with similar to 70% and 95%, respectively, with high-molecular-weight proteins. Cu-metallothionein was not selectively detected (although 28% of Cu was associated with low-molecular-weight proteins). However, information about the specific proteins in the cytosol will require coupling UF with organic mass spectrometry. Data from SPE showed the presence of labile Cu species of similar to 17%, while the fraction of labile Zn species was >55%. However, data from DGT suggested a fraction of labile Cu species only of 7% and a labile Zn fraction of 5%. This data, as compared with previous data from literature, suggests that the DGT technique gave a more plausible estimation of the labile pool of Zn and Cu in cytosol. The combination of results from UF and DGT is capable of contributing to the knowledge about the labile and low-molecular pool of Cu and Zn.en
dc.description.affiliationSao Paulo State Univ UNESP, Environm Studies Ctr CEA, Ave 24 A,1515, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationSao Paulo State Univ UNESP, Inst Geosci & Exact Sci, Dept Geol Basin Studies Lab LEBAC, Ave 24 A,1515, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationSao Paulo State Univ UNESP, Dept Geog & Environm Planning, Ave 24 A,1515, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationSao Paulo State Univ UNESP, Sci & Technol Inst, Dept Environm Engn, Km 137,8, BR-12247004 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Environm Studies Ctr CEA, Ave 24 A,1515, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Inst Geosci & Exact Sci, Dept Geol Basin Studies Lab LEBAC, Ave 24 A,1515, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Dept Geog & Environm Planning, Ave 24 A,1515, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP, Sci & Technol Inst, Dept Environm Engn, Km 137,8, BR-12247004 Sao Jose Dos Campos, SP, Brazil
dc.description.sponsorshipFunda��o de Amparo � Pesquisa do Estado de S�o Paulo (FAPESP)
dc.description.sponsorshipCoordena��o de Aperfei�oamento de Pessoal de N�vel Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cient�fico e Tecnol�gico (CNPq)
dc.description.sponsorshipPetrobras
dc.description.sponsorshipFunda��o para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipIdFAPESP: 2021/00572-0
dc.description.sponsorshipIdCNPq: 308090/2019-5
dc.description.sponsorshipIdFUNDUNESP: 5900.0111820.19.9-Item 35
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1093/mtomcs/mfad018
dc.identifier.citationMetallomics. Oxford: Oxford Univ Press, v. 15, n. 4, 8 p., 2023.
dc.identifier.doi10.1093/mtomcs/mfad018
dc.identifier.issn1756-5901
dc.identifier.urihttp://hdl.handle.net/11449/245619
dc.identifier.wosWOS:000969807500001
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofMetallomics
dc.sourceWeb of Science
dc.subjectcytosol
dc.subjectfish liver
dc.subjectmetals
dc.subjectdiffusive gradient in thin films
dc.subjectsolid phase extraction
dc.subjectultrafiltration
dc.titleCombining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus)en
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dcterms.rightsHolderOxford Univ Press

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