Cell growth on 3D microstructured surfaces

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dc.contributor.authorAraujo, W. W.R.
dc.contributor.authorTeixeira, F. S.
dc.contributor.authorDa Silva, G. N.
dc.contributor.authorSalvadori, D. M.F. [UNESP]
dc.contributor.authorSalvadori, M. C.
dc.contributor.authorBrown, I. G.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFederal University of Ouro Preto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionLawrence Berkeley National Laboratory
dc.date.accessioned2018-12-11T16:41:43Z
dc.date.available2018-12-11T16:41:43Z
dc.date.issued2016-06-01
dc.description.abstractChinese Hamster Ovary (CHO) cell cultures were grown on surfaces lithographed with periodic 3D hexagonal microcavity array morphology. The range of microcavity size (inscribed circle diameter) was from 12 μm to 560 μm. CHO cells were grown also on flat surfaces. The characterization was performed with respect to cell growth density (number of nuclei per unit area) by fluorescence optical microscopy and evaluated by correlation function analysis. We found that optimum microcavity radius was 80 μm, concerning to the maximum cell growth density, being even greater than the growth density on a flat (unstructured) substrate of the same material. This finding can be important for optimization of biotechnological processes and devices.en
dc.description.affiliationInstitute of Physics University of São Paulo, C.P. 66318
dc.description.affiliationPharmacy School UFOP Federal University of Ouro Preto
dc.description.affiliationBotucatu Medical School UNESP São Paulo State University
dc.description.affiliationLawrence Berkeley National Laboratory, 1 Cyclotron Road
dc.description.affiliationUnespBotucatu Medical School UNESP São Paulo State University
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: CNPq-157021/2011-4
dc.format.extent686-689
dc.identifierhttp://dx.doi.org/10.1016/j.msec.2016.03.026
dc.identifier.citationMaterials Science and Engineering C, v. 63, p. 686-689.
dc.identifier.doi10.1016/j.msec.2016.03.026
dc.identifier.file2-s2.0-84962667239.pdf
dc.identifier.issn0928-4931
dc.identifier.scopus2-s2.0-84962667239
dc.identifier.urihttp://hdl.handle.net/11449/168546
dc.language.isoeng
dc.relation.ispartofMaterials Science and Engineering C
dc.relation.ispartofsjr1,110
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiomaterials
dc.subjectCell aggregation
dc.subjectOptical microscopy
dc.subjectSurface patterning
dc.titleCell growth on 3D microstructured surfacesen
dc.typeArtigo

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