Bone repair access of boneceramic™ in 5-mm defects: Study on rat calvaria

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dc.contributor.authorFabris, André Luis da Silva [UNESP]
dc.contributor.authorFaverani, Leonardo Perez [UNESP]
dc.contributor.authorGomes-Ferreira, Pedro Henrique Silva [UNESP]
dc.contributor.authorPolo, Tárik Ocon Braga [UNESP]
dc.contributor.authorSantiago-Júnior, Joel Ferreira
dc.contributor.authorOkamoto, Roberta [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade do Sagrado Coração
dc.date.accessioned2018-12-11T17:17:27Z
dc.date.available2018-12-11T17:17:27Z
dc.date.issued2018-01-01
dc.description.abstractObjective: The aim of this study was to evaluate the osteoconductive potential of BoneCeramic™ on bone healing in rat calvaria 5-mm defects. Material and Methods: A 5-mm calvaria bone defect was induced in three groups and the defect was not filled with biomaterial [Clot Group (CG)], autogenous bone (AG), or Bone Ceramic Group (BCG). Animals were euthanized after 14 or 28 days and the bone tissue within the central area of the bone defect was evaluated. Results were compared using ANOVA and Tukey test (p<0.05). Immunohistochemistry was performed using primary antibodies against osteocalcin, RUNX-2, TRAP, VEGF proteins, and 3-dimensional images of the defects in µCT were obtained to calculate bone mineral density (BMD). Results: In BCG, the defect was completely filled with biomaterial and new bone formation, which was statistically superior to that in the GC group, at both time-points (p<0.001 for 14 days; p=0.002 for 28 days). TRAP protein showed weak, RUNX-2 showed a greater immunolabeling when compared with other groups, VEGF showed moderate immunostaining, while osteocalcin was present at all time-points analyzed. The µCT images showed filling defect by BCG (BMD= 1337 HU at 28 days). Conclusion: Therefore, the biomaterial tested was found to be favorable to fill bone defects for the reporting period analyzed.en
dc.description.affiliationUniv. Estadual Paulista (UNESP) Faculdade de Odontologia de Araçatuba Departamento de Cirurgia e Clínica Integrada
dc.description.affiliationUniversidade do Sagrado Coração Departamento de Ciências da Saúde
dc.description.affiliationUniv. Estadual Paulista (UNESP) Faculdade de Odontologia de Araçatuba Departamento de Ciências Básicas
dc.description.affiliationUnespUniv. Estadual Paulista (UNESP) Faculdade de Odontologia de Araçatuba Departamento de Cirurgia e Clínica Integrada
dc.description.affiliationUnespUniv. Estadual Paulista (UNESP) Faculdade de Odontologia de Araçatuba Departamento de Ciências Básicas
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: #2012/06309-0
dc.identifierhttp://dx.doi.org/10.1590/1678-7757-2016-0531
dc.identifier.citationJournal of Applied Oral Science, v. 26.
dc.identifier.doi10.1590/1678-7757-2016-0531
dc.identifier.fileS1678-77572018000100401.pdf
dc.identifier.issn1678-7765
dc.identifier.issn1678-7757
dc.identifier.lattes1527011976590326
dc.identifier.scieloS1678-77572018000100401
dc.identifier.scopus2-s2.0-85040979077
dc.identifier.urihttp://hdl.handle.net/11449/175776
dc.language.isoeng
dc.relation.ispartofJournal of Applied Oral Science
dc.relation.ispartofsjr0,645
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiocompatible materials
dc.subjectBone regeneration
dc.subjectImmunohistochemistry
dc.titleBone repair access of boneceramic™ in 5-mm defects: Study on rat calvariaen
dc.typeArtigo
unesp.author.lattes1527011976590326

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Artigos - Cirurgia e Clínica Integrada - FOA
Artigos - Ciências Básicas - FOA