ItemArtigoRole of junctional epithelium in maintaining dento-gingival adhesion and periodontal health(2023-01-01) Theodoro, Letícia Helena [UNESP]; Garcia, Valdir Gouveia [UNESP]; Ervolino, Edilson [UNESP]; Holcroft, James; McCulloch, Christopher A.; Ganss, Bernhard; Universidade Estadual Paulista (UNESP); Latin American Institute of Dental Research and Education (ILAPEO); University of TorontoThe dento-gingival junction comprises multiple epithelia including the junctional epithelium (JE), which is the most coronally-located structural element of the dento-gingival junction that demarcates external from internal periodontal environments. After tooth eruption into the oral cavity, a specialized basal lamina is formed that provides firm attachment of the JE to the enamel. This attachment prevents microbial species and oral debris from entering subjacent periodontal tissues. Here we discuss the expression of certain JE adhesion molecules and enamel proteins that maintain the health of the dento-gingival junction but that are perturbed in the pathogenesis of periodontitis. We also consider how evolutionary processes have influenced the development of the JE as a specialized adhesion that is well-suited for protection of the dento-gingival junction. A detailed understanding of the biology of the JE will deepen current models of dento-gingival adhesion, potentially clarify inter-patient variability of susceptibility to periodontitis and help to identify new roles of enamel proteins in periodontal regeneration. ItemArtigoSingle intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis(2023-12-01) Polo, Tárik Ocon Braga [UNESP]; Fonseca-Santos, João Matheus [UNESP]; Momesso, Gustavo Antonio Correa [UNESP]; da Silva, William Phillip Pereira [UNESP]; Barbosa, Stefany [UNESP]; Santos, Anderson Maikon de Souza [UNESP]; Silva, Mirela Caroline [UNESP]; Garcia, Valdir Gouveia [UNESP]; Theodoro, Letícia Helena [UNESP]; Faverani, Leonardo P. [UNESP]; Universidade Estadual Paulista (UNESP); University of Santo AmaroThis study aimed to evaluate the effect of infrared laser (IRL) on bone repair in ovariectomized rats subjected to femoral osteotomies. Of 32 rats, half underwent bilateral ovariectomy (OVX) and the other half underwent sham ovariectomy (SHAM). A period of 3 months was defined to observe the presence of osteoporosis. The rats were subjected to osteotomies in the femurs and then fixed with a miniplate and 1.5-mm system screws. Thereafter, half of the rats from both SHAM and OVX groups were not irradiated, and the other half were irradiated by IRL using the following parameters: wavelength, 808 nm; power, 100 mW; 60 s for each point; 6 J/point; and a total of 5 points of bone gap. All animals were euthanized 60 days after surgery. The femur gap was scanned using micro-computed tomography (micro-CT). The samples were then examined under a confocal laser microscope to determine the amounts of calcein and alizarin red. The slides were stained with alizarin red and Stevenel’s blue for histometric analysis. In the micro-CT analysis, the OVX groups had the lowest bone volume (P < 0.05). When the laser was applied to the OVX groups, bone turnover increased (P < 0.05). New bone formation (NBF) was comparable between SHAM and OVX/IR (P > 0.05) groups; however, it was less in the OVX groups (P < 0.05). In conclusion, the results encourage the use of IRL intraoperatively as it optimizes bone repair, mainly in animals with low bone mineral density. ItemArtigoEffectiveness of antimicrobial photodynamic therapy mediated by butyl toluidine blue in preventing medication-related osteonecrosis of the jaws in rats(2022-12-01) Ervolino, Edilson [UNESP]; Olivo, Mariane Bocalon [UNESP]; Toro, Luan Felipe [UNESP]; Freire, Jéssica de Oliveira Alvarenga [UNESP]; Ganzaroli, Vinícius Franzão [UNESP]; Guiati, Isabella Zacarin [UNESP]; Nuernberg, Marta Aparecida Alberton [UNESP]; Franciscon, João Paulo Soares [UNESP]; Ângelo Cintra, Luciano Tavares [UNESP]; Garcia, Valdir Gouveia; Wainwright, Mark; Theodoro, Leticia Helena [UNESP]; Universidade Estadual Paulista (UNESP); Latin American Institute of Dental Research and Education (ILAPEO); Liverpool John Moores UniversityBackground: Medication-related osteonecrosis of the jaws (MRONJ) is difficult to treat, therefore, prevention would be the ideal clinical approach. This study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT), mediated by butyl toluidine blue (BuTB) in the prevention of MRONJ-like lesions after tooth extraction in rats. Methods: Twenty-eight senescent female rats were distributed in groups: VEH and VEH-aPDT, treated with vehicle, ZOL and ZOL-aPDT, treated with 100 µg/Kg of zoledronate, both treatments every three days over seven weeks. After three weeks from the commencement of treatment, the mandibular first molar was extracted. For the VEH and ZOL groups, no local treatment was performed, while with the VEH-aPDT and ZOL-aPDT groups, photodynamic treatment was carried out at 0, 2, and 4 days after extraction. For aPDT, 500μl of BuTB solution was deposited on the dental extraction site (0.5 mg/ml; 60 s), followed by irradiation with low-level laser (InGaAIP; 660 nm; 35 mW; 74.2 J/cm²; 60 s). After 28 postoperative days, euthanasia was performed. The hemimandibles were processed to: (1) histological analysis of tissue repair; (2) histometric analysis of the percentage of newly formed bone tissue (PNFBT) and percentage of non-vital bone tissue (PNVBT); (3) immunohistochemical analysis for tartrate-resistant acid phosphatase (TRAP). Results: The ZOL and ZOL-aPDT groups showed less TRAP-positive cells when compared with VEH and VEH-aPDT. The ZOL group demonstrated great compromise in the tissue repair process, consistent with MRONJ-like lesions. VEH, VEH-aPDT and ZOL-aPDT presented a favorable tissue repair process. PNFBT in the ZOL group was lower than in the VEH, VEH-aPDT and ZOL-aPDT groups, whereas PNVBT in the ZOL group was higher than in the VEH, VEH-aPDT and ZOL-aPDT groups. Conclusion: aPDT mediated by BuTB prevented the occurrence of MRONJ-like lesions after tooth extraction in rats. ItemArtigoIn vitro dentin permeability and tubule occlusion of experimental in-office desensitizing materials(2023-03-01) de Castro Oliveira, Laryssa [UNESP]; Marchetti, Vitória Marega [UNESP]; de Souza e Silva Ramos, Fernanda [UNESP]; Delbem, Alberto Carlos Botazzo [UNESP]; Souza, Marina Trevelin; Ganss, Bernhard; Theodoro, Leticia Helena [UNESP]; Fagundes, Ticiane Cestari [UNESP]; Universidade Estadual Paulista (UNESP); Universidade Federal de São Carlos (UFSCar); University of TorontoObjectives: This study investigates the dentin permeability (by hydraulic conductance) and tubule occlusion (by confocal and scanning electron microscopies) of in-office desensitizing materials. Materials and methods: Bovine dentin blocks were immersed in EDTA to open dentinal tubules. Placebo varnish (PLA), fluoride varnish (FLU), NaF 5% + 5% nanoparticulate sodium trimetaphosphate varnish (TMP), universal adhesive system (SBU), S-PRG filler varnish (SPRG), Biosilicate (BIOS), and amelotin (AMTN) solution were the materials tested. After application, the specimens underwent an erosive-abrasive challenge. Dentin permeability was evaluated at T0 (initial), T1 (after treatment), and T2 (after challenge). Confocal and scanning electron microscopy (SEM) were used to evaluate, respectively, length and number of dentinal tubule occlusions and opened dentinal tubules, after challenge. Permeability and SEM data were analyzed by two-way repeated measures ANOVA and Tukey’s tests. Confocal data were analyzed by one-way ANOVA, Tukey’s test, and Kruskal–Wallis and Dunn’s tests. Spearman and Pearson’s correlation tests were also used. Significance level was set at 5%. Results: At T1, the AMTN group showed the lowest permeability value, following the increasing order at T2: AMTN = SBU < BIOS = SPRG < TMP < FLU < PLA. The SBU group had the highest value of occluded dentinal tubule length. The AMTN group presented more occluded dentinal tubules compared to PLA and FLU. AMTN and SBU had the lowest values of opened dentin tubules. Results showed a negative correlation between the analyses. Conclusion: The SBU and AMTN solution were more effective in reducing dentin permeability by occluding dentin tubules. Clinical relevance: All materials reduced permeability after challenge, except fluoride varnish. ItemArtigoIntentional Replantation as Treatment of Extrusive Luxation: A Case Report(2022-09-01) Takamiya, Aline Satie [UNESP]; Figueiredo, Leonardo Raniel [UNESP]; Debortoli, Caio Vinicius Lourenço [UNESP]; Sonoda, Paulo Koji Hara; Costa, Luy de Abreu [UNESP]; Figueiredo, Cássio Messias Beija Flor [UNESP]; Sonoda, Celso Koogi [UNESP]; Universidade Estadual Paulista (UNESP); Medical SchoolAccurate diagnosis, immediate care and proper treatment planning are important factors for the successful treatment of dental traumatic injuries. In extrusive luxation, postponement in treatment may lead to the need for new strategies for the resolution of unwanted consequences. The present case report describes an unusual condition of delayed treatment regarding extrusive luxation of two anterior teeth treated by intentional replantation. An 18-year-old female patient attended the Dental School for the treatment of a traumatic injury to the anterior teeth 5 days after a fall from a bicycle. Clinically, teeth #21 and #22 were extruded in incisal edges for 5 mm from their neighboring teeth, had edematous gingiva, showed grade II mobility and were painful to percussion. Radiographically, the roots were intact and the periodontal ligament space was thickened along its entire length. As immediate repositioning could not be performed, intentional replantation was recommended. After detaching the periodontal ligament, the teeth were extracted and the alveolus was curetted and irrigated with saline solution to remove the already-formed clot. After replantation, the teeth were restrained and the patient was medicated with antibiotics as well as analgesics. After 10 days, the splint was removed, the root canals were instrumented and then, filled with calcium hydroxide. After 30 days, they were completely obturated using gutta-percha. The 5-year follow-up showed root integrity, absence of mobility and normal periodontium. The outcomes of the current case report revealed that when intentional replantation was properly conducted, it could be considered an option for the treatment of extrusive luxation where the teeth were not to be immediately repositioned. ItemArtigoDose–response effect of Montelukast on post-extraction dental socket repair and skeletal phenotype of mice(2023-01-01) Ribeiro, Kim Henderson Carmo [UNESP]; da Silva, Raquel Barroso Parra [UNESP]; Roseno, Ana Carolyna Becher [UNESP]; Barreto, Ana Julia Moreno [UNESP]; Bacelar, Ana Carolina Zucon [UNESP]; Ervolino, Edilson [UNESP]; Duarte, Marco Antônio Húngaro; Fakhouri, Walid D.; Chaves-Neto, Antonio Hernandes [UNESP]; Biguetti, Cláudia Cristina; Matsumoto, Mariza Akemi [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP); The University of Texas at Health Science Center at Houston (UTH); The University of Texas at Rio Grande Valley (UTRGV)Bone metabolism and repair are directly regulated by arachidonic acid metabolites. At present, we analyzed the dose–response effects of a selective cysteinyl leukotriene receptor type-1 antagonist during bone repair after tooth extraction and on non-injured skeleton. Sixty-three 129 Sv/Ev male mice composed the groups: C—Control (saline solution); MTK2—2 mg/Kg of Montelukast (MTK) and MTK4–4 mg/Kg of MTK, daily administered by mouth throughout all experimental periods set at 7, 14, and 21 days post-operative. Dental sockets were analyzed by computed microtomography (microCT), histopathology, and immunohistochemistry. Femurs, L5 vertebra and organs were also removed for observation. Blood was collected for plasma bone and liver markers. Histopathology and microCT analysis revealed early socket repair of MTK2 and MTK4 animals, with significant increased BV/TV at days 14 and 21 compared to C. Higher plasma calcium was detected at days 7 and 21 in MTK4 in comparison to C, while phosphate was significantly increased in MTK2 in the same periods in comparison to C and MTK4. No significant differences were found regarding plasma ALP and TRAP, neither for local TRAP and Runx2 immunolabeling at the healing sockets. Organs did not present histological abnormalities. Increased AST levels have been detected in distinct groups and periods. In general, femur phenotype was improved in MTK treated animals. Collectively, MTK promoted early bone formation after tooth extraction and increased bone quality of femurs and vertebra in a time-dose-dependent manner, and should be considered as an alternative therapy when improved post-extraction socket repair or skeleton preservation is required. ItemArtigoThe Local Release of Teriparatide Incorporated in 45S5 Bioglass Promotes a Beneficial Effect on Osteogenic Cells and Bone Repair in Calvarial Defects in Ovariectomized Rats(2023-02-01) de Araújo, Juliani Caroline Ribeiro [UNESP]; Sobral Silva, Leonardo Alvares [UNESP]; de Barros Lima, Vinicius Almeida [UNESP]; Bastos Campos, Tiago Moreira; Lisboa Filho, Paulo Noronha [UNESP]; Okamoto, Roberta [UNESP]; de Vasconcellos, Luana Marotta Reis [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)With the increase in the population’s life expectancy, there has also been an increase in the rate of osteoporosis, which has expanded the search for strategies to regenerate bone tissue. The ultrasonic sonochemical technique was chosen for the functionalization of the 45S5 bioglass. The samples after the sonochemical process were divided into (a) functionalized bioglass (BG) and (b) functionalized bioglass with 10% teriparatide (BGT). Isolated mesenchymal cells (hMSC) from femurs of ovariectomized rats were differentiated into osteoblasts and submitted to in vitro tests. Bilateral ovariectomy (OVX) and sham ovariectomy (Sham) surgeries were performed in fifty-five female Wistar rats. After a period of 60 days, critical bone defects of 5.0 mm were created in the calvaria of these animals. For biomechanical evaluation, critical bone defects of 3.0 mm were performed in the tibias of some of these rats. The groups were divided into the clot (control) group, the BG group, and the BGT group. After the sonochemical process, the samples showed modified chemical topographic and morphological characteristics, indicating that the surface was chemically altered by the functionalization of the particles. The cell environment was conducive to cell adhesion and differentiation, and the BG and BGT groups did not show cytotoxicity. In addition, the experimental groups exhibited characteristics of new bone formation with the presence of bone tissue in both periods, with the BGT group and the OVX group statistically differing from the other groups (p < 0.05) in both periods. Local treatment with the drug teriparatide in ovariectomized animals promoted positive effects on bone tissue, and longitudinal studies should be carried out to provide additional information on the biological performance of the mutual action between the bioglass and the release of the drug teriparatide. ItemArtigoClinical Assessment of Biphasic Calcium Phosphate in Granules and Paste Forms in Human Maxillary Sinus Bone Augmentation: A Randomized, Split-Mouth Clinical Trial(2023-02-01) Bonardi, João Paulo [UNESP]; Pereira, Rodrigo dos Santos; Mourão, Carlos F.; Coelho Mendes, Bruno [UNESP]; Lowenstein, Adam; Montemezzi, Pietro; Giubilato, Flavio [UNESP]; Okamoto, Roberta [UNESP]; Hochuli-Vieira, Eduardo [UNESP]; Universidade Estadual Paulista (UNESP); University of Grande Rio-UNIGRANRIO; Tufts University School of Dental Medicine; San Raffaele HospitalThe aim of the present study is to compare the biphasic calcium phosphate (BCP) using two different forms—(1) granules and (2) paste—in human maxillary sinus bone reconstruction as a split-mouth study using histomorphometric and immunolabeling for osteocalcin. Ten patients with bilateral maxillary posterior partial edentulism were selected in order to reconstruct bone height. They were divided into two groups: BCPG and BCP-P. After six months of bone healing, biopsies were harvested to assess the new bone formation and immunostaining for osteocalcin. The BCP g group had the following results: mean of bone formation in pristine bone 49.4 ± 21.6%, intermediate 49.4 ± 16.2%, and apical 55.3 ± 21.4%. The group BCP-P had a mean of 41.9 ± 17.3% in the pristine bone region, 37.5 ± 7.8% for intermediate, and 39.0 ± 13.5% for apical. The osteocalcin immunolabeling was high for both groups, demonstrating bone calcification. Thus, the two biomaterials present suitable results for the placement of dental implants. ItemCapítulo de livroBiocompatibility of plant extracts(2020-05-07) Cury, Marina Tolomei Sandoval [UNESP]; Nunes, Gabriel Pereira [UNESP]; Vasques, Ana Maria Veiga [UNESP]; de Almeida, Juliano Milanezi [UNESP]; Matheus, Henrique Rinaldi [UNESP]; dos Reis-Prado, Alexandre Henrique; Dezan Junior, Eloi [UNESP]; Emerenciano Bueno, Carlos Roberto [UNESP]; Universidade Estadual Paulista (UNESP); Universidade Federal de Minas Gerais (UFMG)Inflammation is an inner response of a host to an external agent, encompassing complex mediators and events. The use of antiinflammatory substances is applicable for the treatment of some conditions. The anti-inflammatory property of phytotherapics turns them into therapeutic option to conventional medicine and stimulates studies for use in humans. Herbals are widely used in popular medicine in many countries. However, the effectiveness of many of them remains poorly accessed. So, experimental researches aiming to investigate their pharmaceutical properties are essential to determine these paths and compatibility. This chapter will embrace experiments assessing the biocompatibility of extracts through cell culture, edema induction, subcutaneous tests and associations made with different extracts aiming to enhance the effectiveness of some compounds already used in daily practice. In addition, several promising plant extracts with scientifically proved results will be described. ItemCapítulo de livroOther applications of plant extracts in dentistry(2020-05-07) Martins, Christine Men; Quintino Trizzi, Juliana [UNESP]; de Souza Batista, Victor Eduardo [UNESP]; Piazza, Bruno; Matheus, Henrique Rinaldi [UNESP]; Dezan Junior, Eloi [UNESP]; Emerenciano Bueno, Carlos Roberto [UNESP]; University of Western Sao Paulo (UNOESTE); Universidade Estadual Paulista (UNESP); University of Western São Paulo (UNOESTE)Alternative treatments or coadjutants should be studied and proposed, especially considering the global trend and boosting the world health organization towards natural substances and medicinal plants, regarding their advantages as a function of its diversity, flexibility, accessibility, availability and wide acceptance. In this context, current researches have evaluated the effectiveness of natural substances, medicinal plants, phytoterapy and homeopathics: (1) as storage media for avulsed teeth or root surface treatment before teeth replantation; (2) as coadjutant to improve osteointegration and reduce peri-implantitis in dental implants; (3) as an alternative for anesthetic agents; (4) within treatment for bone remodeling during orthodontic movement or rapid maxillary expansion; (5) as hemostatic agents; and (6) as dental plaque disclosing agents. This chapter will discuss several studies regarding to all these related topics. ItemCapítulo de livroPhytotherapy in periodontics(2020-05-07) Vasques, Ana Maria Veiga [UNESP]; Matheus, Henrique Rinaldi [UNESP]; Gusman, David Jonathan Rodrigues [UNESP]; Cury, Marina Tolomei Sandoval [UNESP]; dos Reis-Prado, Alexandre Henrique; Quintino Trizzi, Juliana [UNESP]; Emerenciano Bueno, Carlos Roberto [UNESP]; Universidade Estadual Paulista (UNESP); University of Western São Paulo (UNOESTE); Universidade Federal de Minas Gerais (UFMG)Periodontal diseases continue to be the most commonly occurring oral diseases in modern times. Plaque-induced gingivitis and periodontitis are caused by the penetration of pathogens from the bacterial plaque into the connective tissue, resulting in an inflammatory condition. Gingivitis affects the connective tissue of the gingiva, while periodontitis affects the entire periodontal apparatus. Many adjuvant therapeutic modalities have been tested for the treatment of these conditions. Among them, herbal therapies have shown anti-inflammatory, antioxidant and antimicrobial properties. These phytotherapic are incorporated into mouth rinses, dentifrices, chewing gums, adhesives, and gels in order to prevent and even treat gingivitis. With respect to periodontitis, gels, chips and extracts containing herbs have provided many benefits when used as adjuvants to scaling and root planning. In addition, its benefits are also observed in wound healing following periodontal surgery. Hence, this chapter will present aspects of phytotherapy in periodontics. ItemArtigoWhich Histometric Analysis Approach Is More Reliable for Assessing Histological Bone Tissue Samples?(2022-10-01) Pereira, Rodrigo dos Santos; Mourão, Carlos Fernando; Piattelli, Adriano; Romanos, Georgios E.; Coelho Mendes, Bruno [UNESP]; Giubilato, Flavio [UNESP]; Montemezzi, Pietro; Júnior Conforte, Jadson [UNESP]; Griza, Geraldo Luiz [UNESP]; Bonardi, João Paulo [UNESP]; Hochuli-Vieira, Eduardo [UNESP]; University of Grande Rio-UNIGRANRIO; Tufts University School of Dental Medicine; University “G. D’Annunzio” of Chieti-Pescara; Stony Brook University; Universidade Estadual Paulista (UNESP); Federal Fluminense University; San Raffaele HospitalThis study aims to evaluate the grid of Merz and ImageJ methods for histometric quantification, verifying which is more reliable and defining which is most suitable based on the time required to perform. Thirty histological samples of maxillary sinuses grafted with xenografts were evaluated using an optical light microscope attached to an image capture camera and connected to a microcomputer. The images were digitalized and recorded as a TIFF image, and the new bone formation was evaluated using the grid of Merz and ImageJ. The Bland–Altman analysis was used to identify the agreement between the methods and determine suitable future research options. The timing of the quantification was also performed to identify a possible advantage. The mean value for the quantification analysis timing for the grid of Merz was 194.9 ± 72.0 s and for ImageJ was 871.7 ± 264.4, with statistical significance between the groups (p = 0.0001). The Bland–Altman analysis demonstrated a concordance between the methods, due to the bias being next to the maximum concordance (−1.25) in addition to the graphic showing the scattering points next to the mean of differences and inside of limits of agreement. Thus, it was demonstrated that the grid of Merz presents reliable outcomes and advantages over the ImageJ methodology regarding the time spent to contour the areas of interest. ItemArtigoAntimicrobial photodynamic therapy in the treatment of periodontal diseases(Wiley-Blackwell, 2015-01-01) Garcia, Valdir Gouveia [UNESP]; Theodoro, Leticia Helena [UNESP]; DeFreitas, P. M.; Simoes, A.; Universidade Estadual Paulista (UNESP) ItemArtigoSurgical and non-surgical treatment of periodontal diseases(Wiley-Blackwell, 2015-01-01) Theodoro, Leticia Helena [UNESP]; Garcia, Valdir Gouveia [UNESP]; DeFreitas, P. M.; Simoes, A.; Universidade Estadual Paulista (UNESP) ItemArtigoSalivary flow and salivary proteins in male and female children with autism spectrum disorder: pilot study(Universidade Estadual Paulista Júlio de Mesquita Filho, 2022-12-05) Souza, Matheus Morcela De [UNESP]; Rodrigues, João Victor Soares [UNESP]; Gonçalves, Maria Eduarda Cabrerizo [UNESP]; Theodoro, Letícia Helena [UNESP]; Nakamune, Ana Claudia De Melo Stevanato [UNESP]; Universidade Estadual Paulista (UNESP) ItemArtigoImpact of parental care on oral health of children with Intellectual Disabilities from zero to six years of age(Universidade Estadual Paulista Júlio de Mesquita Filho, 2022-12-16) Paino-sant’ana, Amanda [UNESP]; Stein, Maria Cristina Ruiz Voms [UNESP]; Rodrigues, João Victor Soares [UNESP]; Mulinari-santos, Gabriel [UNESP]; Duque, Cristiane [UNESP]; Zito, Ana Rita Albuquerque [UNESP]; Santos, Marisha De Oliveira [UNESP]; Theodoro, Letícia Helena [UNESP]; Universidade Estadual Paulista (UNESP) ItemArtigoEffect of nonsteroidal anti-inflammatory drugs (NSAIDs) association on physicochemical and biological properties of tricalcium silicate-based cement(2022-05-01) Oliveira, Maria Carolina Guiotti de; Queiroz, Índia Olinta de Azevedo; Machado, Thiago [UNESP]; Garrido, Lorena de Mello Alcântara; Oliveira, Sandra Helena Penha de [UNESP]; Duarte, Marco Antonio Hungaro; Universidade de São Paulo (USP); Universidade Estadual Paulista (UNESP)The aim of this study was to investigate the physicochemical and biological properties of an experimental tricalcium silicate-based repair cement containing diclofenac sodium (CERD). For the physicochemical test, MTA, Biodentine and CERD were mixed and cement disc were prepared to evaluate the setting time and radiopacity. Root-end cavity were performed in acrylic teeth and filled with cements to analyze the solubility up to 7 days. Polyethylene tubes containing cements were prepared and calcium ions and pH were measured at 3h, 24h, 72h and 15 days. For the biological test, SAOS-2 were cultivated, exposed to cements extracts and cell proliferation were investigated by MTT assay at 6h, 24h and 48h. Polyethylene tubes containing cements were implanted into Wistar rats. After 7 and 30 days, the tubes were removed and processed for histological analyses. Parametric and nonparametric data were performed. No difference was identified in relation to setting time, radiopacity and solubility. Biodentine released more calcium ion than MTA and CERD; however, no difference between MTA and CERD were detected. Alkaline pH was observed for all cements and Biodentine exhibited highest pH. All cements promoted a raise on cell proliferation at 24h and 48h, except CERD at 48h. Biodentine stimulated cell metabolism in relation to MTA and CERD while CERD was more cytotoxic than MTA at 48h. Besides, no difference on both inflammatory response and mineralization ability for all cement were found. CERD demonstrated similar proprieties to others endodontic cements available. ItemArtigoEffect of systemic antibiotic therapy on pulp repair following extrusive luxation and avulsion in a murine model: A histomorphological study(2022-01-01) Queiroz, Alfredo Franco [UNESP]; Hidalgo, Mirian Marubayashi; Consolaro, Alberto; Panzarini, Sônia Regina [UNESP]; França, Alline Batistussi [UNESP]; Melo, Moriel Evangelista [UNESP]; Poi, Wilson Roberto [UNESP]; Universidade Estadual de Maringá (UEM); Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP); Centro Universitário de MaringáBackground/Aim: The evidence for any benefit of systemic antibiotic therapy on pulp survival following extrusive luxation and avulsion is inconclusive. The aim of this study was to evaluate the effect of systemic antibiotic therapy with amoxycillin and tetracycline for 7 days on different aspects of dental pulp repair in a murine model. Material and Methods: The right maxillary incisor of 180 4 to 8-week-old male Wistar rats underwent extrusive luxation or avulsion. The animals were then treated with saline solution (control), tetracycline, or amoxicillin administered intra-gastrically for 7 days, and euthanized at 7, 15, and 30 days post-operatively. The layer of odontoblast cells, reparative dentin deposition, Hertwig's epithelial root sheath, pulp necrosis, and periapical inflammatory infiltrate were histomorphologically analyzed and scored. Mann–Whitney, Kruskal–Wallis, and Dunn tests were used to compare mean scores for the different procedures, treatments, and times (p ≤.05). Results: Significant differences were observed between luxated and avulsed teeth (p <.05). In luxated teeth, no differences were observed among treatments and times, except for more tertiary dentin deposition in the coronal third with the use of tetracycline compared with amoxicillin at 15 days (p <.05). In avulsed teeth, higher scores for the layer of odontoblast cells and lower scores for periapical inflammatory infiltrate at 7 days and pulp necrosis at 7 and 15 days were observed in the amoxicillin group compared to the saline and tetracycline groups (p <.05). At 30 days, however, no differences were observed among treatments. Conclusions: The use of systemic antibiotic therapy with tetracycline or amoxicillin for 7 days did not contribute to pulp repair following extrusive luxation or avulsion in rats. However, the results warrant further research into the use of amoxicillin for a more extended period of time in cases of avulsion. ItemArtigoRelationship of bruxism with oral health-related quality of life and facial muscle pain in dentate individuals(2022-01-01) Turcio, Karina-Helga-Leal [UNESP]; Melo Neto, Clóvis-Lamartine de Moraes [UNESP]; Pirovani, Beatriz-Ommati [UNESP]; Santos, Daniela-Micheline dos [UNESP]; Guiotti, Aimée-Maria [UNESP]; Bertoz, André-Pinheiro de Magalhães [UNESP]; Brandini, Daniela-Atili [UNESP]; Universidade Estadual Paulista (UNESP)Background: To determine whether there is a correlation of bruxism (sleep, daytime, or both) with oral health-related quality of life and facial pain of muscular origin in dentate individuals. Materials and Methods: Seventy-four dentate patients (complete dentition) were included in this study. These individuals had pain in the facial muscles due to temporomandibular disorder (TMD). Smokers; and those with obstructive sleep apnea, TMD of joint origin associated or not with pain, malocclusion, and cancer; and users of illicit drugs, psychiatric medications, and alcohol were excluded. Obstructive sleep apnea, bruxism (of sleep and/or daytime), facial muscle pain, and oral health-related quality of life were assessed by the following questionnaires: Berlin Questionnaire, Pintado et al. questionnaire, VAS (Visual Analog Scale) facial muscle pain questionnaire, and Oral Health Impact Profile – 14. Four groups were created: 1) no bruxism; 2) sleep bruxism; 3) daytime bruxism; and 4) sleep and daytime bruxism. Spearman’s correlation test was applied to verify if there was a correlation between the collected data. P values less than 0.05 were considered statistically significant. Results: There was a positive correlation of daytime bruxism with mean pain in the last 3 months (P<0.05) and the worst pain experienced in the last 3 months (P<0.05). Conclusions: Bruxism (sleep, daytime, or both) showed a positive correlation with lower oral health-related quality of life (P<0.05) ItemArtigoTherapeutic Strategies in the Management of Nonresorbable Membrane and Titanium Mesh Exposures Following Alveolar Bone Augmentation: A Systematic Scoping Review(2022-01-01) Poli, Pier Paolo; Beretta, Mario; Maiorana, Carlo; Souza, Francisley Ávila [UNESP]; Bovio, Andrea; Manfredini, Mattia; Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico; University of Milan; Universidade Estadual Paulista (UNESP)Purpose: The exposure of nonresorbable membranes following alveolar bone augmentation is one of the most frequently occurring complications. This review aimed to investigate the treatments that can be adopted to manage the exposure of polytetraﬂuoroethylene-based membranes (PTFE-ms) and titanium meshes (TMs) and their outcomes. Materials and Methods: Two independent reviewers electronically and manually searched the EMBASE, PubMed/MEDLINE, Scopus, and Cochrane bibliographic databases to retrieve pertinent articles available between January 2000 and March 2021. Only human studies describing the type of treatment and the soft tissue outcome following exposure of PTFE-ms or TMs were included. Results: Overall, 11 articles in the PTFE-ms group and 24 in the TM group were included for data analysis. Results indicated that, in both groups, two distinct therapeutic strategies are mostly applied in case of exposure, namely, pharmacologic and mechanical treatments. Other options have been identiﬁed seldomly. Statistically signiﬁcant evidence of an association between the type of barrier membrane and the exposure rate (28.7% vs 38.5% for TMs and PTFE-ms, respectively; P =.019) and between the type of exposed device and the treatment outcome in terms of removal rate following therapy (11.9% and 44.4% for TMs and PTFE-ms, respectively; P <.001) was noted. Conclusion: In both groups, chlorhexidine applications and meticulous plaque control may lead to improved healing conditions after exposure. Surgical removal of the exposed portion can be considered to promote secondary intention healing. The beneﬁcial effects of systemic antibiotics could not be demonstrated in the management of the exposure but should be evaluated in case of graft infection.