Expression of mRNA Encoding the LH Receptor (LHR) and LHR Binding Protein in Granulosa Cells from Nelore (Bos indicus) Heifers Around Follicle Deviation

Página do item simplificado
dc.contributor.authorEreno, R. L.
dc.contributor.authorLoureiro, B.
dc.contributor.authorCastilho, A. C.S.
dc.contributor.authorMachado, M. F.
dc.contributor.authorPegorer, M. F.
dc.contributor.authorSatrapa, R. A.
dc.contributor.authorNogueira, M. F.G.
dc.contributor.authorBuratini, J.
dc.contributor.authorBarros, C. M.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Vila Velha (UVV)
dc.date.accessioned2022-04-28T19:01:43Z
dc.date.available2022-04-28T19:01:43Z
dc.date.issued2015-01-01
dc.description.abstractThe time at which follicles acquire LHR in bovine granulosa cells is the subject of some controversy among researchers. The main objective of the present study was to assess the mRNA expression of LHR and LRBP (mRNA protein binding), a post-transcriptional suppressor of LHR mRNA expression, in granulosa cells from the two largest follicles around the expected time of follicle deviation in Nelore heifers. First, the interval between ovulation and follicle deviation in 20 Nelore heifers was determined (2.3 ± 0.2 days after ovulation). Ovulation was hormonally synchronized, and then, heifers were slaughtered on days 2, 2.5 and 3 after ovulation (before, during and after, respectively, the expected time of follicle deviation), and granulosa cells from the two largest follicles were collected. The mRNA abundance of an LHR fragment common to all isoforms (total LHR) and LRBP was assessed by real-time RT-PCR, and LHR alternative transcripts were assessed by semiquantitative RT-PCR followed by electrophoresis. LHR mRNA expression was not detected before the expected time of deviation. Total LHR mRNA abundance was greater in the largest follicle and increased from day 2.5 to 3. In contrast, LRBP mRNA was detected starting on day 2 and was more expressed in the second largest follicle on days 2.5 and 3. The present data suggest that the expression of LHR mRNA in bovine granulosa cells is established after follicle deviation and that the lower abundance of LRBP mRNA after the expected time of deviation may contribute to greater expression of LHR in the bovine dominant follicle.en
dc.description.affiliationDepartment of Pharmacology Institute of Bioscience University of São Paulo State
dc.description.affiliationLaboratory of Animal Reproductive Physiology University of Vila Velha (UVV)
dc.description.affiliationDepartment of Biology Science University of São Paulo State
dc.description.affiliationDepartment of Physiology Institute of Bioscience University of São Paulo State
dc.format.extent952-957
dc.identifierhttp://dx.doi.org/10.1111/rda.12614
dc.identifier.citationReproduction in Domestic Animals, v. 50, n. 6, p. 952-957, 2015.
dc.identifier.doi10.1111/rda.12614
dc.identifier.issn1439-0531
dc.identifier.issn0936-6768
dc.identifier.scopus2-s2.0-84946411193
dc.identifier.urihttp://hdl.handle.net/11449/220490
dc.language.isoeng
dc.relation.ispartofReproduction in Domestic Animals
dc.sourceScopus
dc.titleExpression of mRNA Encoding the LH Receptor (LHR) and LHR Binding Protein in Granulosa Cells from Nelore (Bos indicus) Heifers Around Follicle Deviationen
dc.typeArtigo

Arquivos

Coleções

Artigos