Determination of oligoelements in parenteral formulations by planar chromatography and spectrophotometry
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1998-01-01
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Marcel Dekker Inc
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The authors have studied the chromatographic behavior of parenteral preparations for pediatric use containing inorganic cations. After separation and identification by thin-layer chromatography, Mn2+, Zn2+, and Cu2+ were analyzed by a method based on reaction with an appropriate reagent and extraction with an organic solvent which yielded elution and preconcentration, resulting in an appropriate solution for colorimetric quantitation. Cr3+ cation was determined by atomic absorption spectrophotometry after appropriate chromatographic separation, using microcrystalline cellulose (adsorbent) and an acetone:water:hydrochloric acid mixture (80:5:8) as the mobile phase.
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Drug Development and Industrial Pharmacy. New York: Marcel Dekker Inc., v. 24, n. 2, p. 109-114, 1998.
