QuEChERS-based method for pesticides analysis in adipose tissue associated with rat Ovaries

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dc.contributor.authorZamariola, Nathalie [UNESP]
dc.contributor.authorNetto, Pedro Toledo
dc.contributor.authorFranchi, Carla Adriene da Silva [UNESP]
dc.contributor.authorDe Camargo, João Lauro Viana [UNESP]
dc.contributor.authorDe Marchi, Mary Rosa Rodrigues [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Paraná (UFPR)
dc.date.accessioned2018-12-11T17:12:29Z
dc.date.available2018-12-11T17:12:29Z
dc.date.issued2017-01-01
dc.description.abstractThe concomitant exposure to low doses of various pesticides is one of the most relevant issues in human toxicology today. An experimental toxicology study was developed to evaluate the effects of this type of exposure on the reproductive capacity of females of three species of rats that were exposed to mixtures of dicofol, dieldrin, endosulfan and permethrin at low doses (LOAEL and NOAEL). In this context, we have developed a method for determining pesticides in adipose tissue (0.5 g, 49% lipid) associated with the ovaries, based on the QuEChERS (quick, easy, cheap, effective, rugged and safe) strategy. The method quantification limit (LOQ) was 0.5 mg/kg for dicofol and permethrin, 0.05 mg/kg for endosulfan and dieldrin and 0.2 mg/kg for diclorobenzophenone. Mean recoveries ranged from 75% to 93% with a relative standard deviation <13%. The unspecific selectivity (matrix effect) indicates the mandatory use of analytical curves constructed on the matrix extract. All the analyzed samples (53 adipose tissue associated to ovaries) showed residues of dichlorobenzophenone + dicofol, dieldrin and cis-permethrin while trans-permethrin were detected in 40% of the samples but were below the LOQ. The data indicated the bioaccumulation characteristics of these substances.en
dc.description.affiliationAnalytical Chemistry Department Chemistry Institute São Paulo State University UNESP
dc.description.affiliationCentre for Marine Studies (CEM) Universidade Federal do Paraná UFPR
dc.description.affiliationDepartment of Pathology Faculty of Medicine São Paulo State University UNESP
dc.description.affiliationUnespAnalytical Chemistry Department Chemistry Institute São Paulo State University UNESP
dc.description.affiliationUnespDepartment of Pathology Faculty of Medicine São Paulo State University UNESP
dc.format.extent399-406
dc.identifierhttp://dx.doi.org/10.1093/jat/bkx023
dc.identifier.citationJournal of Analytical Toxicology, v. 41, n. 5, p. 399-406, 2017.
dc.identifier.doi10.1093/jat/bkx023
dc.identifier.file2-s2.0-85020178001.pdf
dc.identifier.issn1945-2403
dc.identifier.issn0146-4760
dc.identifier.scopus2-s2.0-85020178001
dc.identifier.urihttp://hdl.handle.net/11449/174700
dc.language.isoeng
dc.relation.ispartofJournal of Analytical Toxicology
dc.relation.ispartofsjr1,065
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleQuEChERS-based method for pesticides analysis in adipose tissue associated with rat Ovariesen
dc.typeArtigo
unesp.author.lattes9163387140787963[3]
unesp.author.orcid0000-0002-2830-2540[3]

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Artigos - Química Analítica - IQ