Improvement of an enzyme immunosorbent assay for detecting antibodies against Dioctophyma renale

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dc.contributor.authorPedrassani, Daniela
dc.contributor.authorNascimento, Adjair Antonio do [UNESP]
dc.contributor.authorAndré, Marcos Rogério [UNESP]
dc.contributor.authorMachado, Rosangela Zacarias [UNESP]
dc.contributor.institutionUniversidade do Contestado (UnC)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:38:03Z
dc.date.available2015-12-07T15:38:03Z
dc.date.issued2015-09-15
dc.description.abstractAn available enzyme-linked immunosorbent assay (ELISA) was studied for the detection of anti-Dioctophyma renale antibodies in the sera of dogs using, detection of parasite eggs in urine sediment as a reference test. ELISA uses a soluble antigenic preparation of esophagus of D. renale and the optimal dilutions of the antigen, serum and conjugate were determined by means of checker board titration, using positive (n=13) and negative (n=27) reference serum. The specificity and sensitivity of the ELISA were 93.8% and 92.3% respectively and the kappa index was good (0.76). These results suggest that ELISA described may prove to be an effective serological test for detecting dogs infected and exposed to this parasite mainly dogs that are not eliminating parasite eggs through their urine.en
dc.description.affiliationDepartment of Veterinary Medicine, Universidade do Contestado (UnC), Rua Roberto Ehlke 86, CEP 89460-000 Canoinhas, SC, Brazil
dc.description.affiliationDepartment of Preventive Veterinary Medicine and Animal Reproduction, School of Agrarian and Veterinary Sciences (FCAV), Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900, Jaboticabal, SP, Brazil
dc.description.affiliationDepartment of Veterinary Pathology, FCAV/UNESP, Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900 Jaboticabal, SP, Brazil.
dc.description.affiliationUnespDepartment of Preventive Veterinary Medicine and Animal Reproduction, School of Agrarian and Veterinary Sciences (FCAV), Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900, Jaboticabal, SP, Brazil
dc.description.affiliationUnespDepartment of Veterinary Pathology, FCAV/UNESP, Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14887-900 Jaboticabal, SP, Brazil.
dc.format.extent435-438
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2015.08.007
dc.identifier.citationVeterinary Parasitology, v. 212, n. 3-4, p. 435-438, 2015.
dc.identifier.doi10.1016/j.vetpar.2015.08.007
dc.identifier.issn1873-2550
dc.identifier.lattes3254990612451836
dc.identifier.lattes9139899895580513
dc.identifier.pubmed26299933
dc.identifier.urihttp://hdl.handle.net/11449/131582
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofVeterinary Parasitology
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectDioctophymaen
dc.subjectDogsen
dc.subjectElisa testen
dc.subjectSerological diagnosisen
dc.titleImprovement of an enzyme immunosorbent assay for detecting antibodies against Dioctophyma renaleen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.
unesp.author.lattes3254990612451836
unesp.author.lattes9139899895580513
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt
unesp.departmentPatologia Veterinária - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias
Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias