The Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus Infection

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dc.contributor.authorAyusso, Gabriela Miranda [UNESP]
dc.contributor.authorLima, Maria Letícia Duarte [UNESP]
dc.contributor.authorda Silva Sanches, Paulo Ricardo [UNESP]
dc.contributor.authorSantos, Igor Andrade
dc.contributor.authorMartins, Daniel Oliveira Silva [UNESP]
dc.contributor.authorda Conceição, Pâmela Jóyce Previdelli [UNESP]
dc.contributor.authorCarvalho, Tamara [UNESP]
dc.contributor.authorda Costa, Vivaldo Gomes [UNESP]
dc.contributor.authorBittar, Cíntia [UNESP]
dc.contributor.authorMerits, Andres
dc.contributor.authorSantos-Filho, Norival Alves [UNESP]
dc.contributor.authorCilli, Eduardo Maffud [UNESP]
dc.contributor.authorJardim, Ana Carolina Gomes [UNESP]
dc.contributor.authorde Freitas Calmon, Marilia [UNESP]
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionThe Rockefeller University
dc.contributor.institutionUniversity of Tartu
dc.date.accessioned2023-07-29T16:15:28Z
dc.date.available2023-07-29T16:15:28Z
dc.date.issued2023-05-01
dc.description.abstractChikungunya virus (CHIKV) and Zika virus (ZIKV) are important disease-causing agents worldwide. Currently, there are no antiviral drugs or vaccines approved to treat these viruses. However, peptides have shown great potential for new drug development. A recent study described (p-BthTX-I)2K [(KKYRYHLKPF)2K], a peptide derived from the Bothropstoxin-I toxin in the venom of the Bothrops jararacussu snake, showed antiviral activity against SARS-CoV-2. In this study, we assessed the activity of this peptide against CHIKV and ZIKV and its antiviral action in the different stages of the viral replication cycle in vitro. We observed that (p-BthTX-I)2K impaired CHIKV infection by interfering with the early steps of the viral replication cycle, reducing CHIKV entry into BHK-21 cells specifically by reducing both the attachment and internalization steps. (p-BthTX-I)2K also inhibited the ZIKV replicative cycle in Vero cells. The peptide protected the cells against ZIKV infection and decreased the levels of the viral RNA and the NS3 protein of this virus at viral post-entry steps. In conclusion, this study highlights the potential of the (p-BthTX-I)2K peptide to be a novel broad-spectrum antiviral candidate that targets different steps of the replication cycle of both CHIKV and ZIKV.en
dc.description.affiliationInstitute of Biosciences Letters and Exact Sciences São Paulo State University, SP
dc.description.affiliationSchool of Pharmaceutical Sciences São Paulo State University, SP
dc.description.affiliationInstitute of Biomedical Sciences Federal University of Uberlândia, MG
dc.description.affiliationLaboratory of Molecular Immunology The Rockefeller University
dc.description.affiliationInstitute of Technology University of Tartu
dc.description.affiliationInstitute of Chemistry São Paulo State University, SP
dc.description.affiliationUnespInstitute of Biosciences Letters and Exact Sciences São Paulo State University, SP
dc.description.affiliationUnespSchool of Pharmaceutical Sciences São Paulo State University, SP
dc.description.affiliationUnespInstitute of Chemistry São Paulo State University, SP
dc.identifierhttp://dx.doi.org/10.3390/v15051168
dc.identifier.citationViruses, v. 15, n. 5, 2023.
dc.identifier.doi10.3390/v15051168
dc.identifier.issn1999-4915
dc.identifier.scopus2-s2.0-85160603859
dc.identifier.urihttp://hdl.handle.net/11449/250014
dc.language.isoeng
dc.relation.ispartofViruses
dc.sourceScopus
dc.subjectantiviral
dc.subjectCHIKV
dc.subjectpeptide
dc.subjectZIKV
dc.titleThe Dimeric Peptide (KKYRYHLKPF)2K Shows Broad-Spectrum Antiviral Activity by Inhibiting Different Steps of Chikungunya and Zika Virus Infectionen
dc.typeArtigo
unesp.author.orcid0000-0002-6008-634X[1]
unesp.author.orcid0000-0002-3269-0662[2]
unesp.author.orcid0000-0001-7750-4045[4]
unesp.author.orcid0000-0001-8193-0071[10]
unesp.author.orcid0000-0002-0344-6900[11]
unesp.author.orcid0000-0002-4767-0904[12]
unesp.author.orcid0000-0002-6348-7923[13]
unesp.author.orcid0000-0001-5693-6148[15]

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