Multiple bradykinin-related peptlides from the capture web of the spider Nephila clavipes (Araneae, Tetragnatidae)

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dc.contributor.authorVolsi, ECFR
dc.contributor.authorMendes, M. A.
dc.contributor.authorMarques, M. R.
dc.contributor.authordos Santos, L. D.
dc.contributor.authorSantos, K. S.
dc.contributor.authorde Souza, B. M.
dc.contributor.authorBabieri, E. F.
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:54:26Z
dc.date.available2014-05-20T13:54:26Z
dc.date.issued2006-04-01
dc.description.abstractThree bradykinin-related peptides (nephilakinins-I to -III) and bradykinin itself were isolated from the aqueous washing extract of the capture web of the spider Nephila clavipes by gel permeation chromatography on a Sephacryl S-100 column, followed by chromatography in a Hi-Trap Sephadex-G25 Superfine column. The novel peptides occur-red in low concentrations and were sequenced through ESI-MS/MS analysis: nephilakinin-I (G-P-N-P-G-F-S-P-F-R-NH2), nephilakinin-Il (E-A-P-P-G-F-S-P-F-R-NH2) and nephilakinin-III (P-S-P-P-G-F-S-P-F-R-NH2)- Synthetic peptides replicated the novel bradykinin-related peptides, which were submitted to biological characterizations. Nephilakinins were shown to cause constriction on isolated rat ileum preparations and relaxation on rat duodenum muscle preparations at amounts higher than bradykinin; apparently these peptides constitute B-2-type agonists of ileal and duodenal smooth muscles. All peptides including the bradykinin were moderately lethal to honeybees. These bradykinin peptides may be related to the predation of insects by the webs of N. clauipes. (c) 2005 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, IBRC, CEIS, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUniv São Paulo, InCor, CNPq, MCT,Inst Immunol Invest, São Paulo, SP, Brazil
dc.description.affiliationUniv São Paulo, InCor, FM HC, Discipline Allergy & Immunol, São Paulo, SP, Brazil
dc.description.affiliationUnespUNESP, IBRC, CEIS, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.format.extent690-697
dc.identifierhttp://dx.doi.org/10.1016/j.peptides.2005.08.011
dc.identifier.citationPeptides. New York: Elsevier B.V., v. 27, n. 4, p. 690-697, 2006.
dc.identifier.doi10.1016/j.peptides.2005.08.011
dc.identifier.issn0196-9781
dc.identifier.lattes2901888624506535
dc.identifier.urihttp://hdl.handle.net/11449/19463
dc.identifier.wosWOS:000237047000012
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPeptides
dc.relation.ispartofjcr2.851
dc.relation.ispartofsjr1,001
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectspider toxinspt
dc.subjectbradykinin-related peptidespt
dc.subjectmuscle contraction/relaxationpt
dc.subjectmass spectrometrypeptide sequencingpt
dc.subjectpeptide synthesispt
dc.titleMultiple bradykinin-related peptlides from the capture web of the spider Nephila clavipes (Araneae, Tetragnatidae)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes2901888624506535
unesp.author.orcid0000-0002-2078-9286[2]
unesp.author.orcid0000-0001-5832-1825[4]
unesp.author.orcid0000-0002-7363-8211[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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