Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará
dc.contributor.author | Silva, Jenevaldo B. [UNESP] | |
dc.contributor.author | Lopes, Cinthia T.A. | |
dc.contributor.author | Pinheiro, Cleyton P. | |
dc.contributor.author | Lima, Danilo H.S. | |
dc.contributor.author | Silva, Roberto S.L. | |
dc.contributor.author | Fonseca, Adivaldo H. | |
dc.contributor.author | Araújo, Flábio R. | |
dc.contributor.author | Barbosa-Neto, José D. | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade Federal do Pará (UFPA) | |
dc.contributor.institution | Universidade Federal Rural do Rio de Janeiro (UFRRJ) | |
dc.contributor.institution | Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) | |
dc.date.accessioned | 2014-05-27T11:30:45Z | |
dc.date.available | 2014-05-27T11:30:45Z | |
dc.date.issued | 2013-09-30 | |
dc.description.abstract | The aim of the study was to estimate the prevalence of Babesia bovis and Babesia bigemina in water buffaloes of the Marajó Island, State of Pará, Brazil. We used an indirectenzyme- linked immunosorbent assay (iELISA), with total antigen containing proteins outer surface, and polymerase chain reaction (qPCR), involving the use of SYBR Green based on amplification of a small fragment of the cytochrome b gene. The prevalence of positive animals in iELISA to B. bovis B. bigemina and mixed infection was 24.87% (199/800), 20.75% (166/800) and 18.75% (150/800), respectively. Using the PCR, the presence of B. bovis wasdetected in 15% (18/199) and B. bigemina in 16% (19/199) of animals, and of these, 58% (11/19) presented co-infected by the two agents. The results show a low prevalence of antibodies anti-B. bovis and anti-B. bigemina in water buffaloes from Marajó Island. However, it was observed that the agents of bovine babesiosis circulate in buffaloes, and these may act as reservoirs. | en |
dc.description.affiliation | Laboratório de Imunodiagnóstico Departamento de Patologia Veterinária Facudade de Ciências Agrarias e Veterinarias (FCAV), Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900 | |
dc.description.affiliation | Universidade Federal do Pará Instituto de Medicina Veterinária, Via de Acesso BR 316, Km 62, Bairro Saudade, Castanhal, PA 68740-970 | |
dc.description.affiliation | Laboratório de Doenças Parasitárias Departamento de Epidemiologia e Saúde Pública Universidade Federal Rural de Rio de Janeiro (UFRRJ), BR 465 Km 7, Seropédica, RJ23890-000 | |
dc.description.affiliation | Embrapa Gado de Corte, Rodovia BR 262 Km 4, Campo Grande, MS79002-970 | |
dc.description.affiliationUnesp | Laboratório de Imunodiagnóstico Departamento de Patologia Veterinária Facudade de Ciências Agrarias e Veterinarias (FCAV), Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900 | |
dc.format.extent | 847-850 | |
dc.identifier | http://dx.doi.org/10.1590/S0100-736X2013000700003 | |
dc.identifier.citation | Pesquisa Veterinaria Brasileira, v. 33, n. 7, p. 847-850, 2013. | |
dc.identifier.doi | 10.1590/S0100-736X2013000700003 | |
dc.identifier.file | 2-s2.0-84884579757.pdf | |
dc.identifier.issn | 0100-736X | |
dc.identifier.issn | 1678-5150 | |
dc.identifier.scielo | S0100-736X2013000700003 | |
dc.identifier.scopus | 2-s2.0-84884579757 | |
dc.identifier.uri | http://hdl.handle.net/11449/76671 | |
dc.identifier.wos | WOS:000323339100003 | |
dc.language.iso | por | |
dc.relation.ispartof | Pesquisa Veterinária Brasileira | |
dc.relation.ispartofjcr | 0.385 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | Babesia bigemina | |
dc.subject | Babesia bovis | |
dc.subject | Bubalus bubalis | |
dc.subject | Buffaloes | |
dc.subject | ELISA | |
dc.subject | Marajó Island. | |
dc.subject | QPCR | |
dc.title | Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará | pt |
dc.title.alternative | Molecular and serological prevalence of babesia bovis and babesia bigemina in water buffaloes (bubalus bubalis) on marajo island, state of pará, brazil | en |
dc.type | Artigo | |
dcterms.license | http://www.scielo.br/revistas/pvb/iaboutj.htm | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal | pt |
unesp.department | Patologia Veterinária - FCAV | pt |
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