Toxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulata

dc.contributor.authorMalafaia, Guilherme
dc.contributor.authorAhmed, Mohamed Ahmed Ibrahim
dc.contributor.authorSouza, Sindoval Silva de
dc.contributor.authorRezende, Fernanda Neves Estrela
dc.contributor.authorFreitas, Ítalo Nascimento
dc.contributor.authorda Luz, Thiarlen Marinho
dc.contributor.authorda Silva, Abner Marcelino
dc.contributor.authorCharlie-Silva, Ives
dc.contributor.authorBraz, Helyson Lucas Bezerra
dc.contributor.authorJorge, Roberta Jeane Bezerra
dc.contributor.authorSanches, Paulo R.S. [UNESP]
dc.contributor.authorMendonça-Gomes, Juliana Moreira
dc.contributor.authorCilli, Eduardo M. [UNESP]
dc.contributor.authorAraújo, Amanda Pereira da Costa
dc.contributor.institutionGoiano Federal Institute
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionAssiut University
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFederal University of Ceará
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.abstractThere have been significant impacts of the current COVID-19 pandemic on society including high health and economic costs. However, little is known about the potential ecological risks of this virus despite its presence in freshwater systems. In this study, we aimed to evaluate the exposure of Poecilia reticulata juveniles to two peptides derived from Spike protein of SARS-CoV-2, which was synthesized in the laboratory (named PSPD-2002 and PSPD-2003). For this, the animals were exposed for 35 days to the peptides at a concentration of 40 µg/L and different toxicity biomarkers were assessed. Our data indicated that the peptides were able to induce anxiety-like behavior in the open field test and increased acetylcholinesterase (AChE) activity. The biometric evaluation also revealed that the animals exposed to the peptides displayed alterations in the pattern of growth/development. Furthermore, the increased activity of superoxide dismutase (SOD) and catalase (CAT) enzymes were accompanied by increased levels of malondialdehyde (MDA), reactive oxygen species (ROS) and hydrogen peroxide (H2O2), which suggests a redox imbalance induced by SARS-CoV-2 spike protein peptides. Moreover, molecular docking analysis suggested a strong interaction of the peptides with the enzymes AChE, SOD and CAT, allowing us to infer that the observed effects are related to the direct action of the peptides on the functionality of these enzymes. Consequently, our study provided evidence that the presence of SARS-CoV-2 viral particles in the freshwater ecosystems offer a health risk to fish and other aquatic organisms.en
dc.description.affiliationBiological Research Laboratory Post-Graduation Program in Conservation of Cerrado Natural Resources Goiano Federal Institute, Urutaí Campus, Rodovia Geraldo Silva Nascimento, 2,5km, Zona Rural CEP, GO
dc.description.affiliationPost-Graduation Program in Biotechnology and Biodiversity Goiano Federal Institution and Federal University of Goiás, GO
dc.description.affiliationPost-Graduation Program in Ecology and Conservation of Natural Resources Federal University of Uberlândia, MG
dc.description.affiliationPlant Protection Department Faculty of Agriculture Assiut University
dc.description.affiliationPost-Graduation Programa in Environmental Sciences Federal University of Goiás, GO
dc.description.affiliationDepartment of Pharmacology Institute of Biomedical Sciences University of Sao Paulo, SP
dc.description.affiliationDrug Research and Development Center Federal University of Ceará, CE
dc.description.affiliationDepartment of Physiology and Pharmacology School of Medicine Federal University of Ceará, CE
dc.description.affiliationInstituto de Química Universidade Estadual Paulista, SP
dc.description.affiliationDepartamento de Imunologia Instituto de Ciências Biomédicas Universidade de São Paulo, SP
dc.description.affiliationUnespInstituto de Química Universidade Estadual Paulista, SP
dc.identifier.citationAquatic Toxicology, v. 245.
dc.relation.ispartofAquatic Toxicology
dc.subjectREDOX unbalance
dc.subjectViral particles
dc.subjectWater pollution
dc.titleToxicological impact of SARS-CoV-2 on the health of the neotropical fish, Poecilia reticulataen
dc.typeArtigo 0000-0003-4773-7598 0000-0003-4773-7598[1] 0000-0002-2703-4302[10][13]