Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus)

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dc.contributor.authorPaula, Tassiana Gutierrez de [UNESP]
dc.contributor.authorThomazini Zanella, Bruna Tereza [UNESP]
dc.contributor.authorAlmeida Fantinatti, Bruno Evaristo de [UNESP]
dc.contributor.authorMoraes, Leonardo Nazario de [UNESP]
dc.contributor.authorSilva Duran, Bruno Oliveira da [UNESP]
dc.contributor.authorOliveira, Caroline Bredariol de [UNESP]
dc.contributor.authorSimoes Salomao, Rondinelle Artur [UNESP]
dc.contributor.authorSilva, Rafaela Nunes da [UNESP]
dc.contributor.authorPadovani, Carlos Roberto [UNESP]
dc.contributor.authorSantos, Vander Bruno dos
dc.contributor.authorMareco, Edson Assuncao
dc.contributor.authorCarvalho, Robson Francisco [UNESP]
dc.contributor.authorDal-Pai-Silva, Maeli [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSao Paulo Agcy Agribusiness Technol
dc.contributor.institutionUniv Western Sao Paulo UNOESTE
dc.date.accessioned2018-11-26T17:31:26Z
dc.date.available2018-11-26T17:31:26Z
dc.date.issued2017-05-15
dc.description.abstractSkeletal muscle is capable of phenotypic adaptation to environmental factors, such as nutrient availability, by altering the balance between muscle catabolism and anabolism that in turn coordinates muscle growth. Small noncoding RNAs, known as microRNAs (miRNAs), repress the expression of target mRNAs, and many studies have demonstrated that miRNAs regulate the mRNAs of catabolic and anabolic genes. We evaluated muscle morphology, gene expression of components involved in catabolism, anabolism and energetic metabolism and miRNAs expression in both the fast and slow muscle of juvenile pacu (Piaractus mesopotamicus) during food restriction and refeeding. Our analysis revealed that short periods of food restriction followed by refeeding predominantly affected fast muscle, with changes in muscle fiber diameter and miRNAs expression. There was an increase in the mRNA levels of catabolic pathways components (FBXO25, ATG12, BCL2) and energetic metabolism-related genes (PGC1 alpha and SDHA), together with a decrease in PPAR beta/delta mRNA levels. Interestingly, an increase in mRNA levels of anabolic genes (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) was also observed during food restriction. After refeeding, muscle morphology showed similar patterns of the control group; the majority of genes were slightly up-or down-regulated in fast and slow muscle, respectively; the levels of all miRNAs increased in fast muscle and some of them decreased in slow muscle. Our findings demonstrated that a short period of food restriction in juvenile pacu had a considerable impact on fast muscle, increasing the expression of anabolic (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) and energetic metabolism genes. The miRNAs (miR-1, miR-206, miR-199 and miR-23a) were more expressed during refeeding and while their target genes (IGF-1, mTOR, PGC1 alpha and MAFbx), presented a decreased expression. The alterations in mTORC1 complex observed during fasting may have influenced the rates of protein synthesis by using amino acids from protein degradation as an alternative mechanism to preserve muscle phenotype and metabolic demand maintenance.en
dc.description.affiliationSao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, Botucatu, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Aquaculture Ctr, Jaboticabal, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Inst Biosci Botucatu, Dept Biostat, Botucatu, SP, Brazil
dc.description.affiliationSao Paulo Agcy Agribusiness Technol, Presidente Prudente, SP, Brazil
dc.description.affiliationUniv Western Sao Paulo UNOESTE, Presidente Prudente, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Aquaculture Ctr, Jaboticabal, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Inst Biosci Botucatu, Dept Biostat, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2013/25915-1
dc.description.sponsorshipIdFAPESP: 2014/16949-2
dc.description.sponsorshipIdFAPESP: 2016/00725-3
dc.description.sponsorshipIdCNPq: 447233/2014
dc.description.sponsorshipIdCNPq: 302656/2015-4
dc.format.extent20
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0177679
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 12, n. 5, 20 p., 2017.
dc.identifier.doi10.1371/journal.pone.0177679
dc.identifier.fileWOS000401473500032.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11449/162800
dc.identifier.wosWOS:000401473500032
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleFood restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus)en
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
unesp.author.lattes8727897080522289[9]
unesp.author.orcid0000-0002-7719-9682[9]

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Artigos - Morfologia - IBB
Artigos - Bioestatística - IBB
Jaboticabal - CAUNESP - Centro de Aquicultura da Unesp