Phylogenomic characterization and pangenomic insights into the surfactin-producing bacteria Bacillus subtilis strain RI4914

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dc.contributor.authorFerreira, Julie Kennya de Lima
dc.contributor.authorVarani, Alessandro de Mello [UNESP]
dc.contributor.authorTotola, Marcos Rogerio
dc.contributor.authorAlmeida, Michelle Fernandes
dc.contributor.authorMelo, Dirceu de Sousa
dc.contributor.authorBatista, Cristina Ferreira Silva e
dc.contributor.authorChalfun-Junior, Antonio
dc.contributor.authorOliveira, Kellen Kauanne Pimenta de
dc.contributor.authorRoesch, Luiz Fernando Wurdig
dc.contributor.authorPylro, Victor Satler
dc.contributor.institutionUniversidade Federal de Lavras (UFLA)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionUniv Florida
dc.date.accessioned2022-11-30T13:46:40Z
dc.date.available2022-11-30T13:46:40Z
dc.date.issued2022-09-09
dc.description.abstractBacillus subtilis is a versatile bacterial species able to produce surfactin, a lipopeptide biosurfactant. We carried out the phylogenomic characterization and pangenomic analyses using available B. subtilis complete genomes. Also, we report the whole genome of the biosurfactant-producing B. subtilis strain RI4914 that was isolated from effluent water from an oil exploration field. We applied a hybrid sequencing approach using both long- and short-read sequencing technologies to generate a highly accurate, single-chromosome genome. The pangenomics analysis of 153 complete genomes classified as B. subtilis retrieved from the NCBI shows an open pangenome composed of 28,511 accessory genes, which agrees with the high genetic plasticity of the species. Also, this analysis suggests that surfactin production is a common trait shared by members of this species since the srfA operon is highly conserved among the B. subtilis strains found in most of the assemblies available. Finally, increased surfactin production corroborates the higher srfAA gene expression in B. subtilis strain RI4914.en
dc.description.affiliationFed Univ Lavras UFLA, Dept Biol, Lavras, MG, Brazil
dc.description.affiliationUniv Estadual Paulista, Unesp, Fac Ciencias Agr & Vet, Dept Tecnol, Jaboticabal, SP, Brazil
dc.description.affiliationUniv Fed Vicosa, Dept Microbiol, Lab Biotecnol & Biodiversidade Meio Ambiente, Vicosa, MG, Brazil
dc.description.affiliationUniv Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA
dc.description.affiliationUnespUniv Estadual Paulista, Unesp, Fac Ciencias Agr & Vet, Dept Tecnol, Jaboticabal, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipBrazilian Microbiome Project
dc.description.sponsorshipIdCNPq: 404651/2018-6
dc.description.sponsorshipIdCNPq: 133550/2019-2
dc.description.sponsorshipIdCNPq: 303061/2019-7
dc.description.sponsorshipIdCAPES: 001
dc.format.extent13
dc.identifierhttp://dx.doi.org/10.1007/s42770-022-00815-0
dc.identifier.citationBrazilian Journal Of Microbiology. New York: Springer, 13 p., 2022.
dc.identifier.doi10.1007/s42770-022-00815-0
dc.identifier.issn1517-8382
dc.identifier.urihttp://hdl.handle.net/11449/237851
dc.identifier.wosWOS:000852097400001
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofBrazilian Journal Of Microbiology
dc.sourceWeb of Science
dc.subjectBiosurfactant
dc.subjectNanopore sequencing
dc.subjectPGM IonTorrent
dc.subjectWhole genome sequencing
dc.subjectHybrid assembly
dc.subjectGridIon
dc.titlePhylogenomic characterization and pangenomic insights into the surfactin-producing bacteria Bacillus subtilis strain RI4914en
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.departmentTecnologia - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias