Cellular and Molecular Tissue Response to Triple Antibiotic Intracanal Dressing

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dc.contributor.authorSwerts Pereira, Maristela Soares [UNESP]
dc.contributor.authorRossi, Marcos A.
dc.contributor.authorCardoso, Cristina Ribeiro
dc.contributor.authorSilva, Joao Santana da
dc.contributor.authorBezerra da Silva, Lea Assed
dc.contributor.authorKuga, Milton Carlos [UNESP]
dc.contributor.authorFaria, Gisele [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionJose Rosario Vellano Univ
dc.date.accessioned2014-12-03T13:10:50Z
dc.date.available2014-12-03T13:10:50Z
dc.date.issued2014-04-01
dc.description.abstractIntroduction: The aim of this study was to characterize the response of mouse subcutaneous tissue to triple antibiotic paste (TAP) using conventional light microscopy and real-time PCR (qRT-PCR). Methods: Polyethylene tubes containing TAP or calcium hydroxide (CH) (ie, the control group) were implanted in mouse subcutaneous tissue. Animals that received empty tubes or no tubes were used as additional controls. After periods of 7, 21, and 63 days postimplantation, the specimens were removed and subjected to histologic processing. The number of inflammatory cells and vessels, vessel areas, vascular density, and relative percentage of collagen were evaluated. Gene expression of proinflammatory (interleukin-1 beta, tumor necrosis factor alpha, and interleukin 17) and anti-inflammatory (transforming growth factor beta) cytokines and angiogenic factors (vascular endothelial growth factor and hypoxia-inducible factor-1 alpha) was quantified by 7 and 21 days postimplantation. Results were analyzed using the Student t test, analysis of variance, and the Tukey test (alpha = 0.05). Results: TAP induced an exuberant inflammatory and angiogenic response, with higher numbers of inflammatory cells, higher vascular area and density, and lower relative percentage of collagen compared with CH. In general, the expression of genes involved in inflammation and angiogenesis was higher in the TAP group compared with animals that received CH or empty tubes. Conclusions: The response of mouse subcutaneous tissue to TAP was characterized by exuberant and persistent inflammatory and angiogenic responses with no repair and high gene expression of biomarkers associated with inflammation and angiogenesis.en
dc.description.affiliationUniv Sao Paulo, Dept Paediat Dent Prevent & Community Dent, Ribeirao Preto Dent Sch, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUNESP, Fac Odontol Araraquara, Dept Odontol Restauradora, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationJose Rosario Vellano Univ, Alfenas Dent Sch, Dept Paediat Dent, Unifenas, Alfenas, MG, Brazil
dc.description.affiliationUniv Sao Paulo, Ribeirao Preto Pharmaceut Sci Sch, Dept Clin Anal Toxicol & Food Sci, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Ribeirao Preto Pharmaceut Sci Sch, Dept Biochem & Immunol, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Restorat Dent, Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Odontol Araraquara, Dept Odontol Restauradora, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Restorat Dent, Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipPROPe-UNESP
dc.description.sponsorshipIdFAPESP: 11/19511-0
dc.format.extent499-504
dc.identifierhttp://dx.doi.org/10.1016/j.joen.2013.10.029
dc.identifier.citationJournal Of Endodontics. New York: Elsevier Science Inc, v. 40, n. 4, p. 499-504, 2014.
dc.identifier.doi10.1016/j.joen.2013.10.029
dc.identifier.issn0099-2399
dc.identifier.lattes1627568419811788
dc.identifier.lattes1411074363039082
dc.identifier.urihttp://hdl.handle.net/11449/112562
dc.identifier.wosWOS:000334647400007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Endodontics
dc.relation.ispartofjcr2.886
dc.relation.ispartofsjr1,585
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBiocompatibilityen
dc.subjectcalcium hydroxideen
dc.subjectintracanal dressingen
dc.subjecttriple antibiotic pasteen
dc.titleCellular and Molecular Tissue Response to Triple Antibiotic Intracanal Dressingen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes1627568419811788
unesp.author.lattes1411074363039082
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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