HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”)
dc.contributor.author | Silva, J. Z. | |
dc.contributor.author | Tangerina, M. M.P. [UNESP] | |
dc.contributor.author | Rocha, C. Q. [UNESP] | |
dc.contributor.author | Amaral, F. P. | |
dc.contributor.author | Bento, C. C. | |
dc.contributor.author | Tahira, L. S. [UNESP] | |
dc.contributor.author | Vilegas, W. [UNESP] | |
dc.contributor.author | Sannomiya, M. | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | Universidade Federal de Rondônia | |
dc.date.accessioned | 2023-07-29T16:05:56Z | |
dc.date.available | 2023-07-29T16:05:56Z | |
dc.date.issued | 2016-01-01 | |
dc.description.abstract | The analysis by HPLC-PDA, PCA and the injection of standard compounds contributed considerably for the evaluation of the secondary metabolites of four commercial samples of Camellia sinensis. All analyzed samples, except sample number 2, when extracted with ethanol-water mixture, presented catechin derivatives, fenolic acids, methylxanthines and flavonoids, which are commonly found in the species under study. It is possible that sample number 2 does not correspond to C. sinensis or may have been collected in a specific time when the main metabolites were not being produced. Quantification by UPLC-ESI-TqD-MS/ MS of the caffeine content the samples of C. sinensis showed that the values obtained in this work vary between 2.72 to 7.02 g/100 g of crude extract. Moreover, all samples analyzed showed a higher content of caffeine than those previously reported in the literature, except sample number 1. Analysis by PCA of the total ions of the samples showed that sample number 3 infusion is quite different from the other samples. These data allow us to infer that caffeine can be a taxonomic marker in the samples of C. sinensis. However, its presence cannot be conclusive in the quality control of a medicinal plant, since the total composition of caffeine may vary significantly and, therefore, and its consumption may not provide the expected efficacy. | en |
dc.description.affiliation | Universidade de São Paulo School of Arts Science and Humanities, SP | |
dc.description.affiliation | Universidade Estadual Paulista “Júlio de Mesquita Filho”, Campus of São Paulo Coast | |
dc.description.affiliation | Universidade Federal de Rondônia, Campus de Rolim de Moura | |
dc.description.affiliationUnesp | Universidade Estadual Paulista “Júlio de Mesquita Filho”, Campus of São Paulo Coast | |
dc.format.extent | 636-643 | |
dc.identifier | http://dx.doi.org/10.1590/1983-084X/0115 | |
dc.identifier.citation | Revista Brasileira de Plantas Medicinais, v. 18, n. 3, p. 636-643, 2016. | |
dc.identifier.doi | 10.1590/1983-084X/0115 | |
dc.identifier.issn | 1983-084X | |
dc.identifier.issn | 1516-0572 | |
dc.identifier.scopus | 2-s2.0-85148291983 | |
dc.identifier.uri | http://hdl.handle.net/11449/249665 | |
dc.language.iso | por | |
dc.relation.ispartof | Revista Brasileira de Plantas Medicinais | |
dc.source | Scopus | |
dc.subject | Camellia sinensis | |
dc.subject | HPLC-PDA | |
dc.subject | TLC | |
dc.subject | UPLC-ESI-TqD-MS/MS | |
dc.title | HPLC-PDA and UPLC-ESI-TqD-MS/MS chromatographic analyses of commercial samples of Camellia sinensis (L.) Kuntze (“green tea”) | en |
dc.title | Análises cromatográficas por HPLC-PDA e UPLC–ESI-TqD-MS/MS de amostras comerciais de Camellia sinensis (L.) Kuntze (“chá verde”) | pt |
dc.type | Artigo | |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicente | pt |
unesp.department | Ciências Biológicas - IBCLP | pt |