Comparison of the photodynamic fungicidal efficacy of methylene blue, toluidine blue, malachite green and low-power laser irradiation alone against Candida albicans

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dc.contributor.authorSouza, Rodrigo C. [UNESP]
dc.contributor.authorJunqueira, Juliana Campos [UNESP]
dc.contributor.authorRossoni, Rodnei D. [UNESP]
dc.contributor.authorPereira, Cristiane A. [UNESP]
dc.contributor.authorMunin, Egberto
dc.contributor.authorJorge, Antonio O. C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Camilo Castelo Branco (UNICASTELO)
dc.date.accessioned2014-05-20T14:04:32Z
dc.date.available2014-05-20T14:04:32Z
dc.date.issued2010-05-01
dc.description.abstractThis study was to evaluate specific effects of photodynamic therapy (energy density 15.8 J/cm(2), 26.3 J/cm(2) and 39.5 J/cm(2)) using methylene blue, toluidine blue and malachite green as photosensitizers and low-power laser irradiation on the viability of Candida albicans. Suspensions of C. albicans containing 10(6) cells/ml were standardized in a spectrophotometer. For each dye, 120 assays, divided into four groups according to the following experimental conditions, were carried out: laser irradiation in the presence of the photosensitizer; laser irradiation only; treatment with the photosensitizer only; no exposure to laser light or photosensitizer. Next, serial dilutions were prepared and seeded onto Sabouraud dextrose agar for the determination of the number of colony-forming units per milliliter (CFU/ml). The results were subjected to analysis of variance and the Tukey test (P < 0.05). Photodynamic therapy using the photosensitizers tested was effective in reducing the number of C. albicans.. The number of CFU/ml was reduced by between 0.54 log(10) and 3.07 log(10) and depended on the laser energy density used. Toluidine blue, methylene blue and malachite green were effective photosensitizers in antimicrobial photodynamic therapy against C. albicans, as was low-power laser irradiation alone.en
dc.description.affiliationSão Paulo State Univ UNESP, Sch Dent Sao Jose dos Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationCamilo Castelo Branco Univ UNICASTELO, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Sch Dent Sao Jose dos Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 06/54896-1
dc.format.extent385-389
dc.identifierhttp://dx.doi.org/10.1007/s10103-009-0706-z
dc.identifier.citationLasers In Medical Science. Artington: Springer London Ltd, v. 25, n. 3, p. 385-389, 2010.
dc.identifier.doi10.1007/s10103-009-0706-z
dc.identifier.issn0268-8921
dc.identifier.lattes0322020541055900
dc.identifier.urihttp://hdl.handle.net/11449/22641
dc.identifier.wosWOS:000275444500011
dc.language.isoeng
dc.publisherSpringer London Ltd
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofjcr1.949
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectPhotodynamic therapyen
dc.subjectLow-power laser irradiationen
dc.subjectCandida albicansen
dc.subjectToluidine blueen
dc.subjectMethylene blueen
dc.subjectMalachite greenen
dc.titleComparison of the photodynamic fungicidal efficacy of methylene blue, toluidine blue, malachite green and low-power laser irradiation alone against Candida albicansen
dc.typeTrabalho apresentado em evento
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer London Ltd
unesp.author.lattes0322020541055900
unesp.author.lattes0053567153623569[6]
unesp.author.orcid0000-0002-1747-6158[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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