Fibrolytic enzyme production of Myceliophthora thermophila M.7.7. using inexpensive carbon sources and mineral nutrients

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Moretti, Marcia Maria De Souza [UNESP]
Bonfa, Emily Colferai [UNESP]
Chierotti, Maria Cecilia Maia [UNESP]
Movio, Ariane Priscila [UNESP]
Silva, Roberto da [UNESP]
Gomes, Eleni [UNESP]

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This study investigated the effect of inexpensive carbon and nitrogen sources on enzyme production by Myceliophthora thermophila M.7.7 in solid-state fermentation. Three kinds of lignocellulosic waste (corn straw, sugarcane bagasse and sugarcane straw) and six nitrogen sources (urea, calcium nitrate, analytical ammonium sulphate, yeast extract, agricultural fertilizer NPK 20-05-20 and fertilizing grade ammonium sulphate) were tested. Some physical-chermical parameters of the fermentation, such as temperature, initial pH and moisture content of the substrate on enzyme production, were evoluated. The maximum activities of xylanase (446.9 U/ml) endoglucanase (94.7 U/ml) and beta-glucosidase (2.8 U/ml) were observed in a mixture of corn straw and wheat bran (1:1 w/w) as the carbon source using fertilizer grade ammonium sulphate as the nitrogen source. This production occurred for an incubation period of 96 h, at 40°C, with initial moisture content of 70% and pH 5.0. These results have significant interest since they could be used for the future production of enzymes in a low-cost industrial process.



Myceliophthora thermophila, solid-state fermentation, xylanase, endoglucanase, beta-glucosidase

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African Journal of Microbiology Research, v. 8, n. 52, p. 4013-4019, 2014.