Use of different carbon sources in cultivation of baker's yeast for production of glycerol-3-phosphate dehydrogenase

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dc.contributor.authorGattas, Edwil Aparecida de Lucca [UNESP]
dc.contributor.authorPeres, Maristela F.S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:39Z
dc.date.available2014-05-27T11:28:39Z
dc.date.issued2013-03-07
dc.description.abstractThe physiological state of yeast cells changes during culture growth as a consequence of environmental changes (nutrient limitations, pH and metabolic products). Cultures that grow exponentially are heterogeneous cell populations made up of cells regulated by different metabolic and/or genetic control systems. The strain of baker's yeast selected by plating commercial compressed yeast was used for the production of glycerol-3- phosphate dehydrogenase. Glycerol-3-phosphate dehydrogenase (GPD) has been widely used in the enzyme assays with diverse compounds of industrial interest, such as glycerol or glycerol phosphate, as well as a number of important bioanalytical applications. Each cell state determines the level of key enzymes (genetic control), fluxes through metabolic pathways (metabolic control), cell morphology and size. The present study was carried out to determine the effects of environmental conditions and carbon source on GPD production from baker's yeast. Glucose, glycerol, galactose and ethanol were used as carbon sources. Glycerol and ethanol assimilations required agitation, which was dependent on the medium volume in the fermentation flask for the greatest accumulation of intracellular GPD. Enzyme synthesis was also affected by the initial pH of the medium and inoculum size. The fermentation time required for a high level of enzyme formation decreased with the inoculum size. The greatest amount of enzyme (0.45 U/ml) was obtained with an initial pH of 4.5 in the medium containing ethanol or glycerol. The final pH was maintained in YP-ethanol, but in the YP-glycerol the final pH increased to 6.9 during growth.en
dc.description.affiliationDepartment of Food and Nutrition School of Pharmaceutical Science São Paulo State University, UNESP, Rodovia Araraquara-Jaú, CEP: 14801-902, São Paulo
dc.description.affiliationUnespDepartment of Food and Nutrition School of Pharmaceutical Science São Paulo State University, UNESP, Rodovia Araraquara-Jaú, CEP: 14801-902, São Paulo
dc.description.sponsorshipPADC/FCF
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.format.extent246-249
dc.identifierhttp://world-food.net/use-of-different-carbon-sources-in-cultivation-of-bakers-yeast-for-production-of-glycerol-3-phosphate-dehydrogenase/
dc.identifier.citationJournal of Food, Agriculture and Environment, v. 11, n. 1, p. 246-249, 2013.
dc.identifier.issn1459-0255
dc.identifier.issn1459-0263
dc.identifier.lattes4006598610021833
dc.identifier.scopus2-s2.0-84874526040
dc.identifier.urihttp://hdl.handle.net/11449/74811
dc.language.isoeng
dc.relation.ispartofJournal of Food, Agriculture and Environment
dc.relation.ispartofsjr0,197
dc.relation.ispartofsjr0,197
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectBaker's yeast
dc.subjectGlycerol-3-phosphate dehydrogenase
dc.subjectInduction
dc.subjectalcohol
dc.subjectcell extract
dc.subjectgalactose
dc.subjectglucose
dc.subjectglycerol
dc.subjectglycerol 3 phosphate dehydrogenase
dc.subjectbiomass
dc.subjectcarbon source
dc.subjectcell culture
dc.subjectculture medium
dc.subjectenzyme assay
dc.subjectenzyme synthesis
dc.subjectfungus growth
dc.subjectgenetic regulation
dc.subjectinoculation
dc.subjectmetabolic regulation
dc.subjectnonhuman
dc.subjectpH
dc.subjectprotein determination
dc.subjectSaccharomyces cerevisiae
dc.titleUse of different carbon sources in cultivation of baker's yeast for production of glycerol-3-phosphate dehydrogenaseen
dc.typeArtigo
dcterms.licensehttp://world-food.net/terms-of-use/
unesp.author.lattes4006598610021833[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAlimentos e Nutrição - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas