Publicação:
Thermal Characterization and Cytotoxicity of Complexes Formed by Papain and Cyclodextrin

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dc.contributor.authorVarca, Gustavo H. C.
dc.contributor.authorAndreo-Filho, Newton
dc.contributor.authorFraceto, Leonardo F. [UNESP]
dc.contributor.authorKaneko, Telma M.
dc.contributor.authorFerraz, Humberto G.
dc.contributor.authorEsteves, Natalia M.
dc.contributor.authorIssa, Michelle G.
dc.contributor.authorMathor, Monica B.
dc.contributor.authorLopes, Patricia S.
dc.contributor.institutionUniv Sorocaba
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInstituto de Pesquisas Energéticas e Nucleares (IPEN)
dc.date.accessioned2014-05-20T15:28:56Z
dc.date.available2014-05-20T15:28:56Z
dc.date.issued2007-12-01
dc.description.abstractPapain is a proteolytic enzyme with restricted applications due to its limited stability. Cyclodextrins are widely used in pharmaceutical formulations once they are able to form complexes with other molecules, improving their stability and bioavailability. The purpose of the present paper was to analyze complexes formed by papain/hydroxypropyl-beta-cyclodextrin and papain/beta-cyclodextrin by thermal analysis and cytotoxicity tests to verify their possible interactions and toxicological behavior. Complex solutions were prepared at different molar ratios and collected as a function of time to be lyophilized and analyzed. Results showed changes in endothermic events and cytotoxicity profiles. A complex formation for both complexes is observed; nevertheless, beta-cyclodextrin was able to change the enzyme characteristics more efficiently.en
dc.description.affiliationUniv Sorocaba, Sorocaba, Brazil
dc.description.affiliationUNESP, Sorocaba, Brazil
dc.description.affiliationUniv São Paulo, São Paulo, Brazil
dc.description.affiliationIPEN CNEN, São Paulo, Brazil
dc.description.affiliationUnespUNESP, Sorocaba, Brazil
dc.format.extent463-475
dc.identifierhttp://dx.doi.org/10.1007/s10867-008-9098-8
dc.identifier.citationJournal of Biological Physics. Dordrecht: Springer, v. 33, n. 5-6, p. 463-475, 2007.
dc.identifier.doi10.1007/s10867-008-9098-8
dc.identifier.issn0092-0606
dc.identifier.urihttp://hdl.handle.net/11449/38650
dc.identifier.wosWOS:000259961000011
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Biological Physics
dc.relation.ispartofjcr1.000
dc.relation.ispartofsjr0,353
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPapainpt
dc.subjectCyclodextrinpt
dc.subjectCytotoxicitypt
dc.subjectDifferential scanning calorimetrypt
dc.subjectFibroblastspt
dc.subjectHuman keratinocytespt
dc.titleThermal Characterization and Cytotoxicity of Complexes Formed by Papain and Cyclodextrinen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
dspace.entity.typePublication
unesp.author.orcid0000-0002-7294-9106[8]
unesp.author.orcid0000-0002-9431-3088[2]
unesp.author.orcid0000-0002-2827-2038[3]
unesp.author.orcid0000-0002-6545-237X[1]
unesp.author.orcid0000-0002-5967-7417[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, Sorocabapt
unesp.departmentEngenharia Ambiental - ICTSpt

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Sorocaba - ICTS - Instituto de Ciência e Tecnologia