How candidate genes respond to aluminum toxicity in Citrus x limonia Osbeck?

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dc.contributor.authorSilva, Carolina M. S. [UNESP]
dc.contributor.authorBanguela-Castillo, Alexander
dc.contributor.authorDomingues, Douglas S. [UNESP]
dc.contributor.authorHabermann, Gustavo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInstituto Biológico
dc.date.accessioned2023-03-02T06:50:34Z
dc.date.available2023-03-02T06:50:34Z
dc.date.issued2022-09-01
dc.description.abstractIn acidic soils, toxic aluminum (Al) inhibits root growth of sensitive species, including Citrus plants. In the Americas, rainfed Citrus plantations are highly dependent on unique rootstocks, such as the ‘Mandarin’ lime (Citrus x limonia Osbeck), which is tolerant to drought although sensitive to Al. It requires yearly lime application to grow on soils that are acidic (pH < 5.0) and rich in Al, especially in central and southeastern areas of Brazil. Despite this scenario, genes that are modulated by Al have not yet been searched in this species. Root apices of ‘Mandarin’ lime plants grown for 60 days in nutrient solutions either with 1480 μM Al3+ or 0 μM Al3+ were analyzed by RNA-seq, and differentially expressed candidate genes were validated by qRT-PCR. We highlight the transcriptional up-regulation of citrate synthase and citrate exudation by MATE (multidrug and toxic compound exudation) channels. Genes related to specialized metabolism, pectin methylesterification, auxin response, defense to biotic and abiotic stresses, cell division, suberin deposition, and nitrate uptake were also up-regulated by Al. The overview of up-regulated genes in ‘Mandarin’ lime not only validates its sensitivity to Al, but also points out targets for future research of Al resistance in this rootstock.en
dc.description.affiliationPrograma de Pós-Graduação em Ciências Biológicas (Biologia Vegetal) Departamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24-A, 1515, SP
dc.description.affiliationCentro de P&D de Sanidade Vegetal Laboratório de Bioquímica Fitopatológica Instituto Biológico, Av. Conselheiro R. Alves, 1252, SP
dc.description.affiliationDepartamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24-A, 1515, SP
dc.description.affiliationUnespPrograma de Pós-Graduação em Ciências Biológicas (Biologia Vegetal) Departamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24-A, 1515, SP
dc.description.affiliationUnespDepartamento de Biodiversidade Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24-A, 1515, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: #2013/11370-3
dc.description.sponsorshipIdCNPq: #307431/2020-7
dc.description.sponsorshipIdCNPq: #474169/2013-8
dc.format.extent409-423
dc.identifierhttp://dx.doi.org/10.1007/s40626-022-00253-1
dc.identifier.citationTheoretical and Experimental Plant Physiology, v. 34, n. 3, p. 409-423, 2022.
dc.identifier.doi10.1007/s40626-022-00253-1
dc.identifier.issn2197-0025
dc.identifier.scopus2-s2.0-85133693228
dc.identifier.urihttp://hdl.handle.net/11449/242021
dc.language.isoeng
dc.relation.ispartofTheoretical and Experimental Plant Physiology
dc.sourceScopus
dc.subjectAl3+
dc.subjectAluminum stress
dc.subjectqRT-PCR
dc.subjectRNA-seq
dc.subject‘Mandarin’ lime
dc.titleHow candidate genes respond to aluminum toxicity in Citrus x limonia Osbeck?en
dc.typeArtigo
unesp.author.orcid0000-0001-8454-2744[4]

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