Evidence of photoinduced lipid hydroperoxidation in Langmuir monolayers containing Eosin Y

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dc.contributor.authorPereira, Lucas S.A. [UNESP]
dc.contributor.authorCamacho, Sabrina A. [UNESP]
dc.contributor.authorMalfatti-Gasperini, Antonio A.
dc.contributor.authorJochelavicius, Karen
dc.contributor.authorNobre, Thatyane M.
dc.contributor.authorOliveira, Osvaldo N.
dc.contributor.authorAoki, Pedro H.B. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCNPEM
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-12-11T16:54:53Z
dc.date.available2018-12-11T16:54:53Z
dc.date.issued2018-11-01
dc.description.abstractPhotodynamic therapy (PDT) efficiency depends on many factors including the incorporation of the photosensitizer (PS) in cell membranes and possible lipid hydroperoxidation. In this study, we show that hydroperoxidation may be photoinduced when eosin Y is incorporated into Langmuir monolayers that serve as cell membrane models. This occurs for Langmuir monolayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), which have unsaturation in their hydrophobic chains. In contrast, light irradiation had no effect on monolayers of saturated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Evidence of hydroperoxidation was obtained from the area increase in eosin-containing DOPC and POPC monolayers upon irradiation, which was accompanied by a decrease in monolayer thickness according to grazing incidence X-ray off-specular scattering (GIXOS) data. Furthermore, the changes in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) induced by irradiation were consistent with hydroperoxide migration toward the lipid hydrophilic heads. In summary, this combination of experimental methods allowed us to determine the effects of eosin Y interaction with cell membrane models under irradiation, which may be associated with the underlying mechanisms of eosin Y as photosensitizer in PDT.en
dc.description.affiliationSchool of Sciences Humanities and Languages São Paulo State University (UNESP)
dc.description.affiliationBrazilian Synchrotron Light Laboratory-LNLS CNPEM, Rua Giuseppe Maximo Scolfaro, 10000
dc.description.affiliationIFSC São Carlos Institute of Physics University of São Paulo (USP)
dc.description.affiliationUnespSchool of Sciences Humanities and Languages São Paulo State University (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2013/14262-7
dc.description.sponsorshipIdFAPESP: 2016/13280-0
dc.description.sponsorshipIdFAPESP: 2017/13404-3
dc.description.sponsorshipIdCNPq: 403713/2016-1
dc.format.extent682-689
dc.identifierhttp://dx.doi.org/10.1016/j.colsurfb.2018.08.002
dc.identifier.citationColloids and Surfaces B: Biointerfaces, v. 171, p. 682-689.
dc.identifier.doi10.1016/j.colsurfb.2018.08.002
dc.identifier.file2-s2.0-85051406776.pdf
dc.identifier.issn1873-4367
dc.identifier.issn0927-7765
dc.identifier.lattes7384168674539702
dc.identifier.orcid0000-0003-4701-6408
dc.identifier.scopus2-s2.0-85051406776
dc.identifier.urihttp://hdl.handle.net/11449/171325
dc.language.isoeng
dc.relation.ispartofColloids and Surfaces B: Biointerfaces
dc.relation.ispartofsjr1,071
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCell membrane models
dc.subjectEosin Y
dc.subjectGIXOS
dc.subjectHydroperoxidation
dc.subjectLangmuir monolayers
dc.subjectPhotosensitizers
dc.subjectPM-IRRAS
dc.titleEvidence of photoinduced lipid hydroperoxidation in Langmuir monolayers containing Eosin Yen
dc.typeArtigo
unesp.author.lattes7384168674539702[7]
unesp.author.orcid0000-0003-4701-6408[7]

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