A modular approach to study protein adsorption on surface modified hydroxyapatite

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dc.contributor.authorOzhukil Kollath, Vinayaraj [UNESP]
dc.contributor.authorVan den Broeck, Freya
dc.contributor.authorFehér, Krisztina
dc.contributor.authorMartins, José C.
dc.contributor.authorLuyten, Jan
dc.contributor.authorTraina, Karl
dc.contributor.authorMullens, Steven
dc.contributor.authorCloots, Rudi
dc.contributor.institutionUniversity of Liège
dc.contributor.institutionFlemish Institute for Technological Research (VITO)
dc.contributor.institutionGhent University
dc.contributor.institutionKatholieke Universiteit Leuven
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:34:47Z
dc.date.available2015-12-07T15:34:47Z
dc.date.issued2015
dc.description.abstractBiocompatible inorganic nano- and microcarriers can be suitable candidates for protein delivery. This study demonstrates facile methods of functionalization by using nanoscale linker molecules to change the protein adsorption capacity of hydroxyapatite (HA) powder. The adsorption capacity of bovine serum albumin as a model protein has been studied with respect to the surface modifications. The selected linker molecules (lysine, arginine, and phosphoserine) can influence the adsorption capacity by changing the electrostatic nature of the HA surface. Qualitative and quantitative analyses of linker-molecule interactions with the HA surface have been performed by using NMR spectroscopy, zeta-potential measurements, X-ray photoelectron spectroscopy, and thermogravimetric analyses. Additionally, correlations to theoretical isotherm models have been calculated with respect to Langmuir and Freundlich isotherms. Lysine and arginine increased the protein adsorption, whereas phosphoserine reduced the protein adsorption. The results show that the adsorption capacity can be controlled with different functionalization, depending on the protein-carrier selections under consideration. The scientific knowledge acquired from this study can be applied in various biotechnological applications that involve biomolecule-inorganic material interfaces.en
dc.description.affiliationDepartment of Chemistry, University of Liège, Liège, Belgium
dc.description.affiliationSustainable Materials Management, Flemish Institute for Technological Research (VITO), Boeretang, Mol, Belgium
dc.description.affiliationDepartment of Organic Chemistry,Ghent University, Ghent, Belgium
dc.description.affiliationDepartment of Metallurgy and Materials EngineeringKatholieke Universiteit Leuven, Heverlee, Belgium
dc.description.affiliationAPTIS, University of Liège, Liège, Belgium
dc.description.affiliationDepartamento de Física, Faculdade de Ciências (FC), Universidade Estadual Paulista (UNESP), Bauru, SP, Brasil
dc.description.affiliationUnespDepartamento de Física, Faculdade de Ciências (FC), Universidade Estadual Paulista (UNESP), Bauru, SP, Brasil
dc.format.extent10497-10505
dc.identifierhttp://dx.doi.org/10.1002/chem.201500223
dc.identifier.citationChemistry (weinheim an der Bergstrasse, Germany), v. 21, n. 29, p. 10497-10505, 2015.
dc.identifier.doi10.1002/chem.201500223
dc.identifier.issn1521-3765
dc.identifier.pubmed26096378
dc.identifier.urihttp://hdl.handle.net/11449/131396
dc.language.isoeng
dc.publisherWiley-VCH Verlag GmbH & Co. KGaA, Weinheim
dc.relation.ispartofChemistry (weinheim an der Bergstrasse, Germany)
dc.relation.ispartofsjr2,265
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectAdsorptionen
dc.subjectAmino acidsen
dc.subjectNMR spectroscopyen
dc.subjectProteinsen
dc.subjectSurface chemistryen
dc.titleA modular approach to study protein adsorption on surface modified hydroxyapatiteen
dc.typeArtigo
dcterms.rightsHolderWiley-VCH Verlag GmbH & Co. KGaA, Weinheim
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências, Baurupt
unesp.departmentFísica - FCpt

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Bauru - FC - Faculdade de Ciências