Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line

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dc.contributor.authorFigueiredo, Nancy Bueno [UNESP]
dc.contributor.authorCestari, Silvia Helena [UNESP]
dc.contributor.authorConde, Sandro Jose [UNESP]
dc.contributor.authorMelo Luvizotto, Renata Azevedo [UNESP]
dc.contributor.authorDe Sibio, Maria Teresa [UNESP]
dc.contributor.authorPerone, Denise [UNESP]
dc.contributor.authorHirata Katayama, Maria Lucia
dc.contributor.authorCarraro, Dirce Maria
dc.contributor.authorBrentani, Helena Paula
dc.contributor.authorBrentani, Maria Mitzi
dc.contributor.authorNogueira, Célia Regina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionAC Camargo Hosp
dc.date.accessioned2014-12-03T13:10:29Z
dc.date.available2014-12-03T13:10:29Z
dc.date.issued2014-01-01
dc.description.abstractIt has been well established that estrogen plays an important role in the progression and treatment of breast cancer. However, the role of triiodothyronine (T-3) remains controversial. We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, induce the expression of genes (PR, TGF-alpha) normally stimulated by estradiol (E-2), and suppress genes (TGF-beta) normally inhibited by E-2. Since T-3 regulates growth hormones, metabolism, and differentiation, it is important to verify its action on other genes normally induced by E-2. Therefore, we used DNA microarrays to compare gene expression patterns in MCF-7 breast adenocarcinoma cells treated with E-2 and T-3. Several genes were modulated by both E-2 and T-3 in MCF-7 cells (Student's t-test, P < 0.05). Specifically, we found eight genes that were differentially expressed after treatment with both E-2 and T-3, including amphiregulin, fibulin 1, claudin 6, pericentriolar material 1, premature ovarian failure 1B, factor for adipocyte differentiation-104, sterile alpha motif domain containing 9, and likely ortholog of rat vacuole membrane protein 1 (fold change > 2.0, pFDR < 0.05). We confirmed our microarray results by real-time PCR. Our findings reveal that certain genes in MCF-7 cells can be regulated by both E-2 and T-3.en
dc.description.affiliationUniv Sao Paulo State UNESP, Botucatu Sch Med, Dept Internal Med, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Med, Dept Radiol, BR-01246903 Sao Paulo, Brazil
dc.description.affiliationAC Camargo Hosp, Res Ctr, BR-01509900 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo State, Dept Clin Med, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Sao Paulo State UNESP, Botucatu Sch Med, Dept Internal Med, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 02/09798-0
dc.format.extent7
dc.identifierhttp://dx.doi.org/10.1155/2014/969404
dc.identifier.citationScientific World Journal. New York: Hindawi Publishing Corporation, 7 p., 2014.
dc.identifier.doi10.1155/2014/969404
dc.identifier.fileWOS000330657200001.pdf
dc.identifier.issn1537-744X
dc.identifier.urihttp://hdl.handle.net/11449/112185
dc.identifier.wosWOS:000330657200001
dc.language.isoeng
dc.publisherHindawi Publishing Corporation
dc.relation.ispartofScientific World Journal
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleEstrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Lineen
dc.typeArtigo
dcterms.rightsHolderHindawi Publishing Corporation
unesp.author.lattes7607038776901890[11]
unesp.author.orcid0000-0003-1876-5418[3]
unesp.author.orcid0000-0001-5667-1418[8]
unesp.author.orcid0000-0002-7354-9518[11]
unesp.author.orcid0000-0001-5192-4682[10]
unesp.author.orcid0000-0002-4014-0660[11]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.departmentClínica Médica - FMBpt

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