Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line
dc.contributor.author | Figueiredo, Nancy Bueno [UNESP] | |
dc.contributor.author | Cestari, Silvia Helena [UNESP] | |
dc.contributor.author | Conde, Sandro Jose [UNESP] | |
dc.contributor.author | Melo Luvizotto, Renata Azevedo [UNESP] | |
dc.contributor.author | De Sibio, Maria Teresa [UNESP] | |
dc.contributor.author | Perone, Denise [UNESP] | |
dc.contributor.author | Hirata Katayama, Maria Lucia | |
dc.contributor.author | Carraro, Dirce Maria | |
dc.contributor.author | Brentani, Helena Paula | |
dc.contributor.author | Brentani, Maria Mitzi | |
dc.contributor.author | Nogueira, Célia Regina [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | AC Camargo Hosp | |
dc.date.accessioned | 2014-12-03T13:10:29Z | |
dc.date.available | 2014-12-03T13:10:29Z | |
dc.date.issued | 2014-01-01 | |
dc.description.abstract | It has been well established that estrogen plays an important role in the progression and treatment of breast cancer. However, the role of triiodothyronine (T-3) remains controversial. We have previously shown its capacity to stimulate the development of positive estrogen receptor breast carcinoma, induce the expression of genes (PR, TGF-alpha) normally stimulated by estradiol (E-2), and suppress genes (TGF-beta) normally inhibited by E-2. Since T-3 regulates growth hormones, metabolism, and differentiation, it is important to verify its action on other genes normally induced by E-2. Therefore, we used DNA microarrays to compare gene expression patterns in MCF-7 breast adenocarcinoma cells treated with E-2 and T-3. Several genes were modulated by both E-2 and T-3 in MCF-7 cells (Student's t-test, P < 0.05). Specifically, we found eight genes that were differentially expressed after treatment with both E-2 and T-3, including amphiregulin, fibulin 1, claudin 6, pericentriolar material 1, premature ovarian failure 1B, factor for adipocyte differentiation-104, sterile alpha motif domain containing 9, and likely ortholog of rat vacuole membrane protein 1 (fold change > 2.0, pFDR < 0.05). We confirmed our microarray results by real-time PCR. Our findings reveal that certain genes in MCF-7 cells can be regulated by both E-2 and T-3. | en |
dc.description.affiliation | Univ Sao Paulo State UNESP, Botucatu Sch Med, Dept Internal Med, BR-18618000 Botucatu, SP, Brazil | |
dc.description.affiliation | Univ Sao Paulo, Sch Med, Dept Radiol, BR-01246903 Sao Paulo, Brazil | |
dc.description.affiliation | AC Camargo Hosp, Res Ctr, BR-01509900 Sao Paulo, Brazil | |
dc.description.affiliation | Univ Sao Paulo State, Dept Clin Med, BR-18618000 Botucatu, SP, Brazil | |
dc.description.affiliationUnesp | Univ Sao Paulo State UNESP, Botucatu Sch Med, Dept Internal Med, BR-18618000 Botucatu, SP, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorshipId | FAPESP: 02/09798-0 | |
dc.format.extent | 7 | |
dc.identifier | http://dx.doi.org/10.1155/2014/969404 | |
dc.identifier.citation | Scientific World Journal. New York: Hindawi Publishing Corporation, 7 p., 2014. | |
dc.identifier.doi | 10.1155/2014/969404 | |
dc.identifier.file | WOS000330657200001.pdf | |
dc.identifier.issn | 1537-744X | |
dc.identifier.uri | http://hdl.handle.net/11449/112185 | |
dc.identifier.wos | WOS:000330657200001 | |
dc.language.iso | eng | |
dc.publisher | Hindawi Publishing Corporation | |
dc.relation.ispartof | Scientific World Journal | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.title | Estrogen-Responsive Genes Overlap with Triiodothyronine-Responsive Genes in a Breast Carcinoma Cell Line | en |
dc.type | Artigo | |
dcterms.rightsHolder | Hindawi Publishing Corporation | |
unesp.author.lattes | 7607038776901890[11] | |
unesp.author.orcid | 0000-0003-1876-5418[3] | |
unesp.author.orcid | 0000-0001-5667-1418[8] | |
unesp.author.orcid | 0000-0002-7354-9518[11] | |
unesp.author.orcid | 0000-0001-5192-4682[10] | |
unesp.author.orcid | 0000-0002-4014-0660[11] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatu | pt |
unesp.department | Clínica Médica - FMB | pt |
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